Lectin histochemistry: a concise practical handbook

Brooks, Susan A.; Leathem, Anthony; Schumacher, U.

doi:10.1016/s0014-5793(97)01138-1

Cited by 35 publications

(44 citation statements)

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“…The lectins described in the literature are isolated mainly from plants and other eukaryotic organisms (Brooks et al, 1997 ;Van Damme et al, 1997 ;Singh et al, 1999).…”

Section: Lectin Selectionmentioning

confidence: 99%

Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems

2001

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“…The lectins described in the literature are isolated mainly from plants and other eukaryotic organisms (Brooks et al, 1997 ;Van Damme et al, 1997 ;Singh et al, 1999).…”

Section: Lectin Selectionmentioning

confidence: 99%

Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems

2001

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“…Sections 5 µm thick were stained with haematoxylin-eosin (H&E), and with alcian blue 8GX pH 1 (AB1.0) at pH 2.5 (AB2.5), as well as with AB2.5/periodic acid-Schiff (AB-PAS) for the detection of mucin carbohydrates. The AB1.0 method stains sulphated glycoconjugates; the AB-PAS reaction visualises neutral and acidic glycoconjugates (Pearse 1972, Brooks et al 1997, Table 1). In addition, terminal mucin sugar residues were characterized with various biotinylated lectins (10 µg ml -1 in 0.1 M PBS, pH 7.2, 30 min at room temperature; lectins and their sugar specificities are listed in Table 1).…”

mentioning

confidence: 99%

Biochemical and histochemical effects of perorally applied endotoxin on intestinal mucin glycoproteins of the common carp Cyprinus carpio

Neuhaus¹,

Marel²,

Caspari³

et al. 2007

Dis. Aquat. Org.

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Mucins are high molecular weight glycoproteins produced by goblet cells and secreted on mucosal surfaces. We investigated biochemical and histochemical properties of intestinal mucins of virus-and parasite-free common carp Cyprinus carpio in response to a single peroral application of endotoxin (lipopolysaccharide = LPS). Intracellular mucins were quantified histochemically by their carbohydrate content and characterized by specific, lectin-based methods. In addition, secreted epithelial (intracellular) and luminal (extracellular) mucins were isolated and separated by downward gel filtration. Carbohydrate and protein content were determined photometrically. Subsequently, terminal glycosylation was characterized by a lectin-binding assay. A peroral endotoxin application altered intestinal secretion and composition of intestinal mucin glycoproteins in common carp. A statistically significant decrease in mature luminal mucins was demonstrated, linked to a new biosynthesis of intracellular mucin glycoproteins. Simultaneous changes in the glycosylation pattern of isolated mucins were found. The intestinal mucosal system is purported to provide a removal mechanism for bacterial noxes by increasing secretion of mucins inducing a flushing-out effect, in combination with altered glycosylation patterns that change adhesion properties. Consequently, pseudofaeces of fish, which are a common sign of intestinal parasitical infections, may also be interpreted as an elimination mechanism for strong bacterial noxes. KEY WORDS: Carp · Mucus · Intestinum · Endotoxin · Lipopolysaccharide Resale or republication not permitted without written consent of the publisherDis Aquat Org 77: [17][18][19][20][21][22][23][24][25][26][27] 2007 in fish are considered to be similar to those of mammals. As in mammals, there is production of secretory vesicles with neutral, acidic or sulphated glycoproteins (Shephard 1994, Bosi et al. 2005. Histological and histochemical studies also indicate that fishes with parasitic infections (like infected mammals) exhibit goblet cell hyperplasia and hypertrophy, whereby as a chronic reaction their intracellular mucins shift towards acidic and sulphated glycoprotein types (George & Nadakal 1981, Dezfuli et al. 2002, Bosi et al. 2005.However, to our knowledge little information is available on the biochemical composition of piscine glycoproteins and their response to enteric bacterial infections. We mimicked bacterial infection by an oral application of endotoxin. Endotoxins are lipopolysaccharide (LPS)-protein complexes synthesized mainly by gram-negative bacteria. LPS is an integrated part of the bacterial membrane. Endotoxin has, at least in mammals, a local and systemic immune-inducing function in inflammation reactions (Raetz 1990, Raetz & Whitfield 2002. Toxic effects are mainly mediated by cell receptors (TLR-4) and leucocyte mediators; however, there are numerous key differences between fish and mammals. In many in vitro studies on cells of lower vertebrates, such as fish, extremely high co...

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“…The ovaries of hyperthyroid rats characteristically showed an enlarged number of hypertrophic corpora 15] lutea, while hypothyroidism induced increased counts of growing and atretic follicles and decreased counts of corpora lutea in association with leucocyte infiltration of the ovarian medulla.…”

Section: General Morphologymentioning

confidence: 95%

“…All lectins and their peroxidase conjugates were prepared by Dr. V. Antonyuk (Lectinotest, Lviv, Ukraine). Carbohydrate determinants were visualized according to the lectin-peroxidase-diaminobenzidine staining protocol [15].…”

Section: Methodsmentioning

confidence: 99%

Structural, functional, and lectin histochemical characteristics of rat ovaries and endometrium in experimental hyper- and hypothyroidism

Lutsyk¹,

Sogomonian²

2012

Folia Histochem Cytobiol.

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Abstract:Twenty lectins with different carbohydrate affinities, including five new lectins purified from fungi, were used for histochemical examination of carbohydrate determinants in rat ovaries and endometrium under experimental hyper-and hypothyroidism. In the ovaries of control rats, lectin binding was detected predominantly in the zona pellucida and corona radiata of growing follicles, luteocytes, and leucocytes. Within the endometrium, lectins reacted strongly with the luminal surface, epitheliocytes and secretions of the uterine glands, pre-decidual cells, leucocytes, and collagen fibers. As revealed on general morphology and estrous cycle studies, hyperthyroidism induced ovarian luteinization, and increased the content of pre-decidual cells and of collagen fibers in the endometrium, with no reliable effect on the estrous cycle. Hypothyroidism was accompanied by retardation of the estrous cycle, enhanced folliculogenesis and leucocyte infiltration of ovarian and endometrial stroma. Both hyper-and hypothyroidism significantly affected the tissue carbohydrates of the ovaries and endometrium: specific and differential redistribution of DMan, LFuc, NeuNAc, DGlcNAc, DGalNAc, and DGal determinants was detected under both pathological conditions. Hyperthyroidism induced more severe alterations of glycoconjugates than hypothyroidism; the endometrium was more susceptible to thyroxin-modulated impairments than the ovaries. These results give new insights into the pathogenetic mechanisms of the effects of thyroid disorders on the female reproductive organs and demonstrate the suitability of lectin histochemistry methods for monitoring the efficacy of hormonal imbalance correction therapy, as well as the applicability of new lectin preparations for the selective labeling of ovarian and endometrial constituents.

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Lectin histochemistry: a concise practical handbook

Cited by 35 publications

References 0 publications

Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems

Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems

Biochemical and histochemical effects of perorally applied endotoxin on intestinal mucin glycoproteins of the common carp Cyprinus carpio

Structural, functional, and lectin histochemical characteristics of rat ovaries and endometrium in experimental hyper- and hypothyroidism

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