Lectin histochemistry of the mast cell: Heterogeneity of rodent and human mast cell populations

Roberts, Ian S.; Jones, Carolyn J.P.; Stoddart, R. W.

doi:10.1007/bf01885784

Cited by 31 publications

(14 citation statements)

References 50 publications

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“…The first segment was fixed in Carnoy's solution, cleared in xylene, and embedded in paraffin blocks. Transverse sections (5 ìm) were cut and stained with Alcian Blue-Safranin O according to Roberts, Jones & Stoddart (1990). Mast cell evaluation in mucosa and sub-mucosa was expressed in number per high-powered field (HPF, using 20 ² objective).…”

Section: Identification Of Mast Cells and Measurement Of Histamine Rementioning

confidence: 99%

Involvement of interleukin‐1, prostaglandins and mast cells in rectal distension‐induced colonic water secretion in rats

Eutamène¹,

Théodorou²,

Vergnolle³

et al. 1998

The Journal of Physiology

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In vivo rectal distension (RD) induces a neurally mediated colonic net water hypersecretion in rats. Interleukin‐1β (IL‐1β) also induces neural colonic water hypersecretion involving the release of prostaglandins (PGs) and a mast cell degranulation in rats. This study investigated in vivo the role of IL‐1, PGs and mast cells in RD‐induced colonic hypersecretion. Proximal colonic net water flux was determined using [14C]polyethylene glycol (PEG) 4000 (mol. wt, 4000) in anaesthetized rats. On strips taken from the distal colon: (i) a histological analysis was performed to determine the number of mucosal mast cells (MMC); and (ii) histamine levels were measured by radioimmunoassay after stimulation with compound 48/80. RD induced a net colonic water secretion that was blocked by i.c.v. administration of IL‐1ra (an IL‐1 receptor antagonist) and indomethacin, and by systemic treatment with doxantrazole and indomethacin. RD decreased the number of resident mast cells and the release of histamine from the distal colonic strips. Moreover, using SDS‐PAGE immunoblotting the expression of IL‐1β was detected in the brain. These results suggest that, in rats, RD induces colonic net water hypersecretion by the activation of a neuro‐immunological reflex pathway, involving brain IL‐1β, PG release and peripheral mast cell degranulation.

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Section: Identification Of Mast Cells and Measurement Of Histamine Rementioning

confidence: 99%

Involvement of interleukin‐1, prostaglandins and mast cells in rectal distension‐induced colonic water secretion in rats

Eutamène¹,

Théodorou²,

Vergnolle³

et al. 1998

The Journal of Physiology

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show abstract

“…4 The affinity of lectins for carbohydrates has been applied to the characterization of mast cell populations in humans, rodents, and dogs. 2,5,14,17,32 Although special stains have been used to diagnose canine mast cell tumors, 1,12,24,31 a systematic comparison has not been done. The purposes of this study Received for publication October 12, 1993. were to evaluate the use of histochemical, affinity, and immunohistochemical stains in formalin-fixed tissues in diagnosing mast cell tumors, especially poorly differentiated tumors, and to determine if the stains would differentiate them from histiocytomas.…”

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confidence: 99%

Canine Mast Cell Tumors: A Comparison of Staining Techniques

Simões

Schoning

1994

J VET Diagn Invest

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Abstract. Twelve histochemical methods; affinity staining with avidin peroxidase, wheat germ agglutinin, and concavalin-A agglutinin; and an immunohistochemical stain with Kpl (CD68) antibody were compared for their relative effectiveness in staining canine mast cell tumors. Stains were compared in 28 mast cell tumors and 19 histiocytomas. The effectiveness of the histochemical methods and the lectins decreased as the mast cells became less differentiated. None of the staining methods were positive on histiocytomas. Periodic acidSchiff (PAS) gave positive results in a few cases of mast cell tumors where other histochemical stains were negative. Although avidin peroxidase and Kpl antibody stained more mast cell tumors than any other method, they did not differ significantly from Luna's method, toluidine blue pH 0.5, toluidine blue pH 4.5, alcian blue pH 2.5, safranin O, Unna's method, and Giemsa. No stain was ideal for the diagnosis of canine mast cell tumors; however, this study suggests that the use of avidin peroxidase, Kpl antibody, and PAS may give additional information for individual poorly differentiated tumors without substantial increase in time or cost.

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“…There is moderately strong membranous, goblet cell and stromal staining with e-PHA in this section from distal colon (e-PHA x 2100). other normal organs and pathological processes (Jones & Stoddart, 1986;McMahon et al, 1989;Roberts et a]., 1990). Other factors, including type and duration of tissue fixation and tissue processing, may also be important in the analysis of such variations.…”

Section: Discussionmentioning

confidence: 99%

Glycoconjugates of the normal human colorectum: a lectin histochemical study

1994

Self Cite

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Previous studies of the normal human colorectum by lectin histochemistry have used a mixture of tissues, including those derived from colons harbouring neoplasia and inflammatory bowel diseases. In the current investigation, tissues from patients without either of these conditions have been examined with a wide panel of lectins, encompassing specificities directed against both N- and O-linked sequences, using an avidin peroxidase revealing system and evaluated with a semiquantitative scoring method. The results of binding of these lectins have been compared with those seen in the resection margins of (at least 5 cm away from) colorectal carcinomas. Consistent regional variations were noted between right- and left-sided colonic tissues, with more diverse glycan structures and a greater sialyl content in the distal colon. There was evidence of graduation of formation of oligosaccharide chains in developing crypts, possibly related to the maturation and expression of glycosyl transferases responsible for the incorporation of mannose residues of N-linked oligosaccharides and of N-acetylgalactosamine and N-acetylglucosamine. Comparison with previous reports has revealed some variations, possibly related to tissue fixation and processing and to lectin concentrations employed, which raises the question of standardization of methodologies in lectin histochemical investigations.

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Lectin histochemistry of the mast cell: Heterogeneity of rodent and human mast cell populations

Cited by 31 publications

References 50 publications

Involvement of interleukin‐1, prostaglandins and mast cells in rectal distension‐induced colonic water secretion in rats

Involvement of interleukin‐1, prostaglandins and mast cells in rectal distension‐induced colonic water secretion in rats

Canine Mast Cell Tumors: A Comparison of Staining Techniques

Glycoconjugates of the normal human colorectum: a lectin histochemical study

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