Lectin purified from Lonchocarpus campestris seeds inhibits inflammatory nociception

Pires, Alana de Freitas; Bezerra, Mirna Marques; Amorim, Renata Morais Ferreira; Nascimento, Francisco Lucas Faustino do; Marinho, Márcia Machado; Moura, Raniere M.; Silva, Mayara Torquato Lima; Correia, Jorge Luis Almeida; Cavada, Benildo Sousa; Assreuy, Ana Maria Sampaio; Nascimento, Kyria S.

doi:10.1016/j.ijbiomac.2018.11.233

Cited by 22 publications

(2 citation statements)

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“…How lectins participate in immune response remain unknown. In plants, lectins from Bryothamnion triquetrum and Lonchocarpus campestris could inhibit inflammatory nociception and anti-inflammatory activity ( 42 – 45 ). In the present study, OppLTL inhibited the activity of the NF-κB signal pathway, suggesting its potential anti-inflammatory activity.…”

Section: Discussionmentioning

confidence: 99%

Immunological characterization and function analysis of L-type lectin from spotted knifejaw, Oplegnathus punctatus

Liu

Wang

et al. 2022

Front. Immunol.

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Lily-type lectin (LTL) plays significant roles in innate immune response against pathogen infection. LTL in animals and plants has received widespread attention. In the present study, an LTL (OppLTL) was identified from spotted knifejaw Oplegnathus punctatus. The OppLTL encoded a typical Ca2+-dependent carbohydrate-binding protein containing a CRD domain. The qRT-PCR showed that it was mainly expressed in the gill and was significantly upregulated after Vibrio anguillarum challenge. The agglutination analysis showed that the recombinant OppLTL could bind and agglutinate Gram-negative and Gram-positive bacteria in a Ca2+-dependent manner. However, the binding activity was different. Meanwhile, the recombinant OppLTL could hemagglutinate mammalian and teleost erythrocytes. Subcellular localization revealed that OppLTL was mainly detected in the cytoplasm of HEK293T cells. The dual-luciferase analysis revealed that OppLTL could inhibit the activity of the NF-κB signal pathway in HEK293T cells after OppLTL overexpression. These findings collectively demonstrated that OppLTL could be involved in host innate immune response and defense against bacterial infection in spotted knifejaw.

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Section: Discussionmentioning

confidence: 99%

Immunological characterization and function analysis of L-type lectin from spotted knifejaw, Oplegnathus punctatus

Liu

Wang

et al. 2022

Front. Immunol.

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“…nociceptive [9], anticancer properties [10,11] and others [12]. Besides the above cited biological activities, some legume lectins are known to elicit an interesting insecticidal effect, most likely due to lectin interactions with glycoconjugates in the insects cells, thus affecting essential biological processes of the organism [13,14].…”

Section: Introductionmentioning

confidence: 99%

A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke

Osterne

Oliveira²,

Schutter³

et al. 2022

Glycoconj J

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The Dalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin from Vataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires divalent cations for its activity. Sugar-inhibition assays demonstrate the lectin specificity towards N-acetyl-D-galactosamine, Dgalactose and related sugars. Furthermore, glycan array analyses show that VaL interacts preferentially with glycans containing terminal GalNAc/Galβ1-4GlcNAc. Biological activity assays were performed using three insect cell lines: CF1 midgut cells from the spruce budworm Choristoneura fumiferana, S2 embryo cells from the fruit fly Drosophila melanogaster, and GutAW midgut cells from the corn earworm Helicoverpa zea. In vitro assays indicated a biostatic effect for VaL on CF1 cells, but not on S2 and GutAW cells. The lectin presented a biostatic effect by reducing the cell growth and inducing cell agglutination, suggesting an interaction with glycans on the cell surface. VaL has been characterized as a galactoside-specific lectin of the Dalbergieae tribe, with sequence similarity to lectins from Vatairea and Arachis.

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Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

Silva

Oliveira

Freitas

et al. 2021

Chemistry & Biodiversity

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Previous studies have characterized a saline extract from Microgramma vacciniifolia rhizome and its lectin (MvRL)‐rich fraction with low acute toxicity. In the present study, we evaluated these preparations for acute toxicity (1,000 mg/kg) and antinociceptive and anti‐inflammatory activities (100–400 mg/kg for the extract and 25–50 mg/kg for the fraction). No signs of toxicity were observed. Both the extract and fraction increased the latency period for nociception in the hot plate assay, decreased writhing induced by acetic acid, and promoted analgesic effects in phases 1 and 2 of the formalin test. The antinociceptive mechanism was attributed to interactions with opioid receptors and K+ ATPase channels. The extract and fraction decreased carrageenan‐induced paw edema in 46.15 % and 77.22 %, respectively, at the highest doses evaluated. Furthermore, the fraction was shown to act on the bradykinin pathway. The ability to decrease leukocyte migration after treatment was also verified in the peritonitis and air pouch models. In exudates collected from air pouches, decreased tumor necrosis factor (TNF)‐α and increased interleukin (IL)‐10 levels were noted. Both the extract and fraction also effectively inhibited the development of granulomatous tissue. In conclusion, the substances investigated in this study can be used for the development of novel therapeutic options for pain and inflammatory processes.

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Lectin purified from Lonchocarpus campestris seeds inhibits inflammatory nociception

Cited by 22 publications

References 21 publications

Immunological characterization and function analysis of L-type lectin from spotted knifejaw, Oplegnathus punctatus

Immunological characterization and function analysis of L-type lectin from spotted knifejaw, Oplegnathus punctatus

A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke

Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

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