Lectin staining of saccharides in the normal and hypothyroid developing organ of Corti

Prieto, Jorge J.; Rueda, Joaquı́n; Sala, Maria L.; Merchán, Jaime A.

doi:10.1016/0165-3806(90)90229-r

Cited by 31 publications

(19 citation statements)

References 19 publications

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“…In summary, there were general correlations between the occurrence of the cytoplasmic glycoconjugates and events during development. The present study clearly shows that the various parts of the TM contained distinctly different glycoconjugates during development and in adults, confirming previous studies (Gil-Loyzaga et al, 1985;Rueda and Lim, 1988;Prieto et al, 1990b;Sugiyama et al, 1992).…”

Section: Tmsupporting

confidence: 92%

“…Our present findings are in agreement with those results, and support the idea that the MTM is produced by cells of the GER and pillar cells, and the mTM is secreted by cells of the LER. Moreover, we demonstrated that the inner por-tion of the GER is involved in the secretion of different glycoconjugates, rather than the outer portion, as was previously reported (Prieto et al, 1990b;Gil-Loyzaga et al, 1991).…”

Section: Tmsupporting

confidence: 80%

“…It has been reported that development of the organ of Corti is impeded in the hypothyroid rat (Uziel et al, 1981(Uziel et al, , 1983. A study by Prieto et al (1990b) in hypothyroid rats demonstrated that the TM failed to lose its reactivity to SBA and succ-WGA, and that the apical surface of the GER cells maintained a strong reactivity to PHA-E and PNA (peanut agglutinin). Similar results have been reported using tritiated carbohydrates in hypothyroid animals (Remezal and Gil-Loyzaga, 1993).…”

Section: Tmmentioning

confidence: 99%

“…The molecular composition of the TM has been widely studied and is known to consist of proteins and carbohydrates (Steel, 1980(Steel, , 1986Tomoda et al, 1984;GilLoyzaga et al, 1985;Khalkhali-Ellis et al, 1987;Tachibana et al, 1987;Hasko and Richardson, 1988;Rueda and Lim, 1988;Fermin et al, 1990;Prieto et al, 1990b;25 AugustMunyer and Schulte, 1991;Sugiyama et al, 1991aSugiyama et al, ,b, 1992Suzuki et al, 1992;Kawamata and Igarashi, 1993;Rubio et al, 1994;Munyer and Schulte, 1995;Gil-Loyzaga, 1997;Bianchi et al, 1999). Thalmann et al (1987) demonstrated that type II collagen is the major constituent of the TM protein.…”

mentioning

confidence: 99%

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Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Rueda¹,

Cantos²,

Lim³

2003

Anat. Rec.

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During development, different epithelial cells in the mouse cochlea express different cell surface glycoconjugates, which may reflect membrane specialization. Some of the lectins tested in this study (SBA, succ-WGA, and PSA) labeled the sensory cells of the cochlea around birth. Other lectins (WGA, Con A, RCA-II, and PHA-E) labeled surfaces of the sensory cells, particularly the stereocilia, from early stages of development (gestation day (GD) 16) through 21 days after birth. These may be adhesion molecules needed to attach the newly forming tectorial membrane (TM) to the stereocilia. Lectin staining of the developing TM revealed that the substructures of the TM are biochemically distinct. Lectin staining also showed the temporal sequence of the expression of cytoplasmic glycoconjugates of the cochlear epithelium during development. Biochemical changes during development are probably the result of different cells being involved in the production of glycoconjugates, and may have functional significance, specifically with regard to the expression of adhesion and/or signaling molecules. Anat Rec Part A 274A: 923-933, 2003.

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Section: Tmsupporting

confidence: 92%

Section: Tmsupporting

confidence: 80%

Section: Tmmentioning

confidence: 99%

mentioning

confidence: 99%

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Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Rueda¹,

Cantos²,

Lim³

2003

Anat. Rec.

Self Cite

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“…This is in agreement with other results reporting an acceleration of myelination in hypothyroid rats, with precocious increases of myelin enzyme activities (41 ). A clear timing control by thyroid hormone also occurs during cerebellar development (42), in the transition between the juvenile and adult forms of Tau (43), in the expression of neuronal markers such as synapsin I in culture (44), during development of the organ of Corti (45), or in the appearance ofenzymes during development ofcholinergic neurons (46).…”

Section: Resultssupporting

confidence: 92%

Neonatal hypothyroidism affects the timely expression of myelin-associated glycoprotein in the rat brain.

Rodrı́guez-Peña

Ibarrola²,

Íñiguez³

et al. 1993

J. Clin. Invest.

159

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Congenital hypothyroidism strongly affects myelination. To assess the role of thyroid hormone on myelin gene expression, we have studied the effect of hypothyroidism on the steady state levels of myelin-associated glycoprotein (MAG) and its mRNA in rat brain during the first postnatal month. As studied by immunoblot analysis of several brain regions, MAG increased from days 10-15 onwards, reaching constant levels by days 20-25. Hypothyroid samples showed a delay in the accumulation of MAG that was more severe in rostral regions, such as cortex and hippocampus. The effect of hypothyroidism on the accumulation of the protein correlated with mRNA levels. MAG mRNA started to accumulate in the cerebrum of normal animals by postnatal day 7, reaching maximal levels by day 20. Hypothyroid rats showed a delay of several days in the onset of mRNA expression, increasing thereafter at the same rate as in normal animals, and eventually reaching similar values. When individual brain regions were analyzed, we found strong regional differences in the effect of hypothyroidism. The cerebral cortex was most affected, with messenger levels lower than in normal animals at all ages. In more caudal regions differences between control and hypothyroid rats were evident only at the earlier stages of myelination, with spontaneous recovery at later ages. By run on analysis, we found no differences in transcriptional activities of the MAG gene in normal, hypothyroid, or T4-treated rats. Therefore, the effects of hypothyroidism on MAG mRNA and protein levels were most likely caused by decreased mRNA stability. We propose that thyroid hormone contributes to enhanced myelin gene expression by affecting the stability of newly transcribed mRNA in the early phases of myelination. (J. Clin. Invest. 1993.812-818.)

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Olivocochlear efferent innervation of the organ of corti in hypothyroid rats

Cantos

López

Merchán

et al. 2003

J of Comparative Neurology

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Congenital hypothyroidism induces developmental abnormalities in the auditory receptor, causing deafness due to a poor development of the outer hair cells (OHCs) and a lack of synaptogenesis between these cells and the olivocochlear axons. This efferent innervation is formed by two separate systems: the lateral system, which originates in the lateral superior olive (LSO) and reaches the inner hair cells; and the medial system, which originates in the ventral nucleus of the trapezoid body (VNTB) and innervates the OHCs. A previous study carried out in our laboratory showed that in congenitally hypothyroid animals, the neurons which give rise to the efferent system are normal in number and distribution, although smaller in size. The aim of the present work was to study the efferent fibers in the auditory receptor of hypothyroid animals, by means of stereotaxic injections of biotinylated dextran amine in the nuclei that give rise to the olivocochlear system: LSO and VNTB. In hypothyroid animals, injections in LSO gave rise to lateral olivocochlear fibers lacking their characteristic dense terminal arbors, while injections in the VNTB-labeled fibers terminating in the spiral bundle region, far from the OHCs with which they normally contact. In the latter case, only a small percentage of labeled fibers reached the OHCs area, giving off only two radial branches maximum. Because the number of neurons which develop into the efferent innervation was normal in hypothyroid animals, we conclude that medial fibers may contact a new target.

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Lectin staining of saccharides in the normal and hypothyroid developing organ of Corti

Cited by 31 publications

References 19 publications

Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Distribution of glycoconjugates during cochlea development in mice: Light microscopic lectin study

Neonatal hypothyroidism affects the timely expression of myelin-associated glycoprotein in the rat brain.

Olivocochlear efferent innervation of the organ of corti in hypothyroid rats

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