Lectins and antibodies to blood group antigens as markers for the basal cells of the human respiratory epithelium

Bals, Robert; Welsch, Ulrich

doi:10.1002/(sici)1097-0029(19970901)38:5<505::aid-jemt7>3.0.co;2-g

Cited by 11 publications

(4 citation statements)

References 17 publications

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“…The latter observation is consistent with previous studies reporting a higher risk of the disease (odds ratio [OR]: 1.23) (Golinelli et al, 2020) and respiratory failure (OR: 1.45) (Ellinghaus et al, 2020) in patients with blood group A. The ABO blood antigens are found on the normal gastric, antral (Matias-Guiu and Guix, 1988), and intestinal mucosae (Fagerberg et al, 2014), as well as on the normal respiratory epithelia (Bals and Welsch, 1997;Fujitani et al, 2000). Furthermore, angiotensin converting enzyme-2 (ACE2), which is bound by the viral S protein, is known to be highly expressed in the small intestine, duodenum, and gallbladder (Fagerberg et al, 2014); and viral nucleocapsid protein has been visualized in the gastric, duodenal, and rectum glandular epithelial cells (Xiao et al, 2020).…”

Section: Discussionsupporting

confidence: 89%

Dynamics of the COVID-19 Clinical Findings and the Serologic Response

et al. 2021

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The factors affecting the dynamics of lengthening of symptoms and serologic responses are not well known. In order to see how the serologic responses change in relation to the clinical features, we selected a group of 472 adults with a positive IgM/IgG antibody test result from a baseline study of the anti-SARS-CoV-2 seropositivity, assessed their COVID-19 and past medical histories, and followed them up in about 3 months. Nearly one-fourth of the subjects were asymptomatic at the baseline; 12.8% subjects became symptomatic at the follow-up (FU) when 39.8% of the subjects had some persisting symptoms. At the baseline, 6.1% showed anti-SARS-CoV-2 IgM positive, 59.3% only for IgG, and 34.5% for both. At the FU, these figures declined to 0.6, 54.0, and 4.4%, respectively, with the mean IgM and IgG levels declining about 6.3 and 2.5 folds. Blood group A was consistently linked to both sustaining and flipping of the gastrointestinal (GI) and respiratory symptoms. The baseline IgM level was associated with GI symptoms and pre-existing cirrhosis in multivariate models. Both of the baseline and FU IgG levels were strongly associated with age, male, and lung involvement seen in chest computed tomography (CT)-scan. Finally, as compared with antibody decayers, IgM sustainers were found to be more anosmic [mean difference (MD): 11.5%; P = 0.047] with lower body mass index (BMI) (MD: 1.30 kg/m2; P = 0.002), while IgG sustainers were more commonly females (MD: 19.2%; P = 0.042) with shorter diarrhea duration in the FU (MD: 2.8 days; P = 0.027). Our findings indicate how the anti-SARS-CoV-2 serologic response and COVID-19 clinical presentations change in relation to each other and basic characteristics.

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Section: Discussionsupporting

confidence: 89%

Dynamics of the COVID-19 Clinical Findings and the Serologic Response

et al. 2021

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“…As for cell-surface molecular markers, α6β4 integrin or CD44 was never detected on human airway epithelial cells dispersed by pronase digestion, thus preventing the discrimination of positive or negative subsets. We also confirmed that G. simplicifolia lectin, a marker for basal cells in the rat airway epithelium [29,37], has no affinity for human airway epithelial cells [38,39].…”

Section: Discussionsupporting

confidence: 69%

Aquaporin‐3 Expression in Human Fetal Airway Epithelial Progenitor Cells

et al. 2005

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Airway epithelium stem cells have not yet been prospectively identified, but it is generally assumed that both secretory and basal cells have the capacity to divide and differentiate. Previously, we developed a test for progenitor cells of the human airway epithelium, relying on the transplantation of fetal respiratory tissues into immunodeficient mice. In this study, we hypothesized that airway-repopulating epithelial progenitors can be marked with surface antigens, and we screened an array of such candidate markers, including lectin ligands, the CD44 and CD166 adhesion molecules, and the aquaporin-3 (AQP3) water channel. We observed that AQP3 is selectively expressed on the surface of basal cells, allowing the separation by flow cytometry of AQP3 + basal cells and AQP3 -ciliated and secretory cells. Functional evaluation of sorted cells in vivo showed that AQP3 + cells can restore a normal pseudostratified, mucociliary epithelium as well as submucosal glands. AQP3 -cells are also endowed with a similar potential, although faster engraftment suggests their inclusion of more committed progenitors. These results show that stem cell candidates in the human tracheo-bronchial mucosa can be positively selected with a novel marker but also, for the first time, that epithelial progenitors exist among both basal and suprabasal cell subsets within the human airway. Stem Cells 2005;23:992-1001

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“…Moreover, CD151 and TF epitopes were still intact after enzymatic cell dissociation, as assessed by immunohistochemistry on cytospun cells (data not shown). In addition to CD151 and TF, we tested GSI‐B4, which has been shown to be specific for basal cells in animals [26] and in human blood B group subjects [27], but in our study, this marker was not expressed on human airway epithelial basal cells (data not shown). Furthermore, AQP3, which has been described both as specific for animal, human adult [28, 29], and human fetal [18] basal cells and as nonspecific for an airway epithelial population [30], recognized all surface epithelial cells in our human airway tissues (data not shown).…”

Section: Resultsmentioning

confidence: 87%

Basal Cells of the Human Adult Airway Surface Epithelium Retain Transit-Amplifying Cell Properties

Hajj

Baranek²,

Naour³

et al. 2006

Stem Cells

139

125

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In numerous airway diseases, such as cystic fibrosis, the epithelium is severely damaged and must regenerate to restore its defense functions. Although the human airway epithelial stem cells have not been identified yet, we have suggested recently that epithelial stem/progenitor cells exist among both human fetal basal and suprabasal cell subsets in the tracheal epithelium. In this study, we analyzed the capacity of human adult basal cells isolated from human adult airway tissues to restore a well-differentiated and functional airway epithelium. To this end, we used the human-specific basal cell markers tetraspanin CD151 and tissue factor (TF) to separate positive basal cells from negative columnar cells with a FACSAria cell sorter. Sorted epithelial cells were seeded into epithelium-denuded rat tracheae that were grafted subcutaneously in nude mice and on collagen-coated porous membranes, where they were grown at the air-liquid interface. Sorted basal and columnar populations were also analyzed for their telomerase activity, a specific transitamplifying cell marker, by the telomeric repeat amplification protocol assay. After cell sorting, the pure and viable CD151/TF-positive basal cell population proliferated on plastic and adhered on epithelium-denuded rat tracheae, as well as on collagen-coated porous membranes, where it was able to restore a fully differentiated mucociliary and functional airway epithelium, whereas viable columnar negative cells did not. Telomerase activity was detected in the CD151/ TF-positive basal cell population, but not in CD151/TFnegative columnar cells. These results demonstrate that human adult basal cells are at least airway surface transitamplifying epithelial cells. STEM CELLS 2007;25:139 -148

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Lectins and antibodies to blood group antigens as markers for the basal cells of the human respiratory epithelium

Cited by 11 publications

References 17 publications

Dynamics of the COVID-19 Clinical Findings and the Serologic Response

Dynamics of the COVID-19 Clinical Findings and the Serologic Response

Aquaporin‐3 Expression in Human Fetal Airway Epithelial Progenitor Cells

Basal Cells of the Human Adult Airway Surface Epithelium Retain Transit-Amplifying Cell Properties

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