Lectins engineered to favor a glycan-binding conformation have enhanced antiviral activity

Matoba, Yasuyuki; Sato, Yoji; Oda, Kosuke; Hatori, Yoshinori; Morimoto, Kanehisa

doi:10.1016/j.jbc.2021.100698

Cited by 7 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is worth noting that the study of Matoba et al [19] increased the antiviral activity of lectins. Such proteins have as characteristics the recognition and maintenance of specific and reversible bonds to mono-or oligosaccharides and other substances containing sugars, maintaining the covalent structure of these glycosidic ligands [19]. They can precipitate cells, glycoconjugates, and polysaccharides from animal, plant, virus, and bacterial sources [20,21].…”

Section: Lectin Activitymentioning

confidence: 94%

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

et al. 2023

View full text Add to dashboard Cite

Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds. Cryogels, which are generated by freezing a reactive polymerization mixture, are highlighted due to their versatility and low relative cost as macroporous, polymeric, monolithic adsorbents. The conversion of cryogels into affinity adsorbents is one possible alternative to their optimal application. Some of the most often utilized supports for immobilizing particular ligands are monolithic columns manufactured with epoxy radicals on their surfaces. The purification of biomolecules with a high degree of specificity, such as lectins and glycoproteins with an affinity for glycosylated groups, has garnered interest in the use of fixed non-traditional beds functionalized with ligands of particular interest. The interaction is both robust enough to permit the adsorption of glycoproteins and reversible enough to permit the dissociation of molecules in response to changes in the solution’s pH. When compared to other protein A-based approaches, this one has been shown to be more advantageous than its counterparts in terms of specificity, ease of use, and cost-effectiveness. Information on polymeric, macroporous, monolithic adsorbents used in the affinity chromatographic purification of lectins has been published and explored.

show abstract

Section: Lectin Activitymentioning

confidence: 94%

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

et al. 2023

View full text Add to dashboard Cite

show abstract

“…For example, the engineered Pseudomonas taiwanensis lectin (PTL) bind high-mannose glycans on the HA protein of the influenza virus, thereby inhibiting the entry of viral particles. 13 BanLec (an engineered plant lectin isolated from the fruit of bananas) is another recombinant lectin with anti-viral activity against HIV, HCV, and influenza virus, all of which have high-mannose-type N-glycans on their surfaces. Because BanLec exhibited a strong T-cell mitogenic response, a recombinant H84T mutant was generated to reduce mitogenicity while preserving the virucidal activity.…”

Section: Recombinant Lectins As Anti-viral Agentsmentioning

confidence: 99%

Recombinant lectins as pioneering anti-viral agents against COVID-19

Saad¹

2021

HTIJ

View full text Add to dashboard Cite

The primary target for vaccine design and anti-viral therapeutics for the deadly severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus disease 2019 (COVID-19) is the coronavirus surface spike (S) glycoprotein. Like other enveloped viruses, S glycoproteins are masked by a dense sugar “coat” of host-derived glycans that mediate immune evasion by molecular mimicry through shielding the immunogenic surface proteins from host immune responses. Paradoxically, this same protective glycan shield can make these sugar-coated viruses vulnerable to immune attack by soluble lectins of the innate immune system that are still able to recognise these glycans as pathogen-associated molecular patterns (PAMPs) leading to complement activation. In reality, recombinant lectins that target virus-associated glycans have the potential to be used as anti-viral agents; and therefore, binding of recombinant lectins to viruses could represent a paradigm shift for viral infection therapy. Likewise, SARS-CoV-2- associated glycans can offer novel targets for recombinant lectins as innovative anti-SARSCoV-2 agents. Unfortunately, pharmacological hurdles currently constrain the entry of recombinant lectins into clinical trials but more vigorous research into potentially useful modifications of these agents can truly develop a new landscape of anti-viral lectin-based therapeutics.

show abstract

“…Lectin-induced mitogenicity can be overcome by attempting glycoengineering techniques such as BanLac, which was engineered to eliminate its mitogenicity by amino acid mutation at position 83–84 from histidine to threonine [19] , without compromising antiviral activity against Ebola and influenza viruses [20] , [21] . In another recent study published in April 2021, a site-specific engineered lectin based on structural insights, Pseudomonas taiwanensis lectin (PTL), reportedly enhanced antiviral activity against the influenza virus and such site-specific engineering of lectins can be a potential strategy to boost the antiviral activity of lectins [22] . The data from 2015 to 2020 showed that numerous potential antiviral lectins were discovered along with studies of structural modifications when needed [23] .…”

Section: Introductionmentioning

confidence: 99%

Plant lectins as prospective antiviral biomolecules in the search for COVID-19 eradication strategies

Ahmed

Jahan

Nissapatorn

et al. 2022

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

Lectins or clusters of carbohydrate-binding proteins of non-immune origin are distributed chiefly in the Plantae. Lectins have potent anti-infectivity properties for several RNA viruses including SARS-CoV-2. The primary purpose of this review is to review the ability of lectins mediated potential biotherapeutic and bioprophylactic strategy against coronavirus causing COVID-19. Lectins have binding affinity to the glycans of SARS-COV-2 Spike glycoprotein that has N-glycosylation sites. Apart from this, the complement lectin pathway is a “first line host defense” against the viral infection that is activated by mannose-binding lectins. Mannose-binding lectins deficiency in serum influences innate immunity of the host and facilitates infectious diseases including COVID-19. Our accumulated evidence obtained from scientific databases particularly PubMed and Google Scholar databases indicate that mannose-specific/mannose-binding lectins (MBL) have potent efficacies like anti-infectivity, complement cascade induction, immunoadjuvants, DC-SIGN antagonists, or glycomimetic approach, which can prove useful in the strategy of COVID-19 combat along with the glycobiological aspects of SARS-CoV-2 infections and antiviral immunity. For example, plant-derived mannose-specific lectins BanLac, FRIL, Lentil, and GRFT from red algae can inhibit and neutralize SARS-CoV-2 infectivity, as confirmed with in-vitro , in-vivo , and in-silico assessments. Furthermore, Bangladesh has a noteworthy resource of antiviral medicinal plants as well as plant lectins. Intensifying research on the antiviral plant lectins, adopting a glyco-biotechnological approach, and with deeper insights into the “glycovirological” aspects may result in the designing of alternative and potent blueprints against the 21st century's biological pandemic of SARS-CoV-2 causing COVID-19

show abstract

Lectins engineered to favor a glycan-binding conformation have enhanced antiviral activity

Cited by 7 publications

References 47 publications

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

Recombinant lectins as pioneering anti-viral agents against COVID-19

Plant lectins as prospective antiviral biomolecules in the search for COVID-19 eradication strategies

Contact Info

Product

Resources

About