LED Light-Activated Hypocrellin B Induces Mitochondrial Damage of Ovarian Cancer Cells

Abstract

Objective. Hypocrellin is a natural photosensitizer from a traditional Chinese herb. In the present study, our aim is to investigate the effect of LED light-activated hypocrellin B on mitochondria of ovarian cancer cells. Material and Methods. Ovarian cancer HO-8910 cells were incubated with hypocrellin B at the concentration of 2.5 µM for 5 h and then irradiated by blue light from a novel LED source. Cell survival rate of HO-8910 cells was measured using MTT assay 24 h after photodynamic treatment of hypocrellin B. Mitochondrial morphology was observed using transmission electron microscopy (TEM). Mitochondrial membrane potential was measured using flow cytometry with JC-1 staining. Results. MTT assay showed that cell survival rate of HO-8910 cells in the photodynamic treatment group has significantly decreased down to 27.22±1.26% (P < 0.01). Light irradiation alone or hypocrellin B alone showed no significant impact. In our TEM mitochondria of the cells after photodynamic treatment of hypocrellin B showed severe damage with swollen mitochondria that had nearly nonexistant cristae. Mitochondrial membrane potential remarkably decreased after photodynamic action of hypocrellin B. Conclusion. The findings demonstrated that photodynamic action of hypocrellin B significantly decreased cell proliferation of ovarian cancer HO-8910 cells, caused severe damage to mitochondrial structure, and induced mitochondrial membrane collapse.

Hypocrellin A-based photodynamic action induces apoptosis in A549 cells through ROS-mediated mitochondrial signaling pathway

“…Fu et al 40 first demonstrated that the photodynamic effect of HA could inhibit the mitochondrial ATPase of hepatoma cells in vitro after irradiation by high-voltage sodium light. Moreover, Jiang et al 41 found that hypocrellin B damaged the mitochondrial structure of ovarian cancer HO-8910 cells after PDT. Recently, Zhao׳s group demonstrated that 17-(3-amino-1-pentanesulfonic acid)-substituted hypocrellin B Schiff-base (PENSHB)-mediated PDT decreased the mitochondrial inner membrane potential, subsequently releasing cytochrome c and inducing apoptotic death 42 .…”

Involvement of the Mitochondria-Caspase Pathway in HeLa Cell Death Induced by 2-Ethanolamino-2-Demethoxy-17-Ethanolimino-Hypocrellin B (EAHB)-Mediated Photodynamic Therapy

“…28 Third, the mitochondrion has been reported to play a key role in the regulation of apoptosis. 16,29 Mitochondrial dysfunction including the loss of mitochondrial membrane potential (DCm), permeability transition, and release of cytochrome c from the mitochondrion into the cytosol, are all associated with apoptosis. 30 In this study, the loss of DCm and release of cytochrome c were observed in EAHB-mediated PDT-treated HeLa cells (Fig- that PDT could open the permeability transition pore, which then leads to the loss of integrity of the outer mitochondrial membrane; this would then release the intermembrane proteinssuch as cytochrome c-into the cytosol.…”

Buck-Boost/Forward Hybrid Converter for PV Energy Conversion Applications

“…In recent years, light emitting diodes (LEDs) are becoming more prevalent in a wide application. Due to material advances over the past few decades, efficiencies of LEDs have increased many times [1], and their applications have rapidly grown for automotive taillights, LCD back lights, traffic signals, and electronic signs [2,3]. Moreover, serious greenhouse effect and environmental pollution caused by overusing fossil fuels have disturbed the balance of global climate.…”