LED phototherapy in full-thickness burns induced by CO2 laser in rats skin

Abstract

Many studies have been conducted on the treatment of burns because they are important in morbidity and mortality. These studies are mainly focused on improving care and quality of life of patients. The aim of this study was evaluate the LED phototherapy effects in rats skin full-thickness burns induced by CO laser. The animals were divided in NT group that did not received any treatment and LED group that received LED irradiation at 685 nm, 220 mW, and 4.5 J/cm during 40 s by burned area. Biopsies were obtained after 7, 14, and 21 days of treatment and submitted to histological and immunohistochemical analysis. The LED phototherapy shows anti-inflammatory effects, improves angiogenesis, and stimulates the migration and proliferation of fibroblasts. The T CD8+ lymphocytes were more common in burned areas compared to T CD4+ lymphocytes since statistically significant differences were observed in the LED group compared to the NT group after 7 days of treatment. These results showed that LED phototherapy performs positive influence in full-thickness burns repair from the healing process modulated by cellular immune response. The obtained results allowed inferring that burns exhibit a characteristic cell immune response and this cannot be extrapolated to other wounds such as incision and wounds induced by punch, among others.

Efeitos fotobiomoduladores das luzes vermelha, azul e verde sobre o colágeno durante o processo de cicatrização de queimaduras cutâneas: uma revisão integrativa

“…Resultados semelhantes foram obtidos por Melo et al (2018), com LED λ= 685nm. Nesse estudo observou-se aumento do número de vasos sanguíneos, proliferação epitelial moderada e fibroblastos, indicando que o LED potencializa a síntese da matriz de tecido conjuntivo.…”

Effect of Green LED Light Irradiation on Bone Marrow-Derived Osteoblast-Like Cells

“…PBMT has various applications and is used for relieving pain, promoting wound healing, and treating neuralgia [3][4][5][6] . In recent years, it has been reported that PBMT promotes wound healing in bone tissue and is also effective for early osseointegration of oral implants [7][8][9] .…”