Abstract. In this paper, we present an isomorphism between the ring of general polynomials over a division algebra D with center F and the group ring of the free monoid with [D : F ] variables over D. Using this isomorphism, we define the characteristic polynomial of any matrix over any division algebra, i.e., a general polynomial with one variable over the algebra whose roots are precisely the left eigenvalues. Furthermore, we show how the left eigenvalues of a 4 × 4 quaternion matrices can be obtained by solving a general polynomial equation of degree 6.