In this paper, we introduce transposition regularity into AG-groupoids, and a variety of transposition regular AG-groupoids (L1/R1/LR, L2/R2/L3/R3-groupoids) are obtained. Their properties and structures are discussed by their decomposition theorems: (1) L1/R1-transposition regular AG-groupoids are equivalent to each other, and they can be decomposed into the union of disjoint Abelian subgroups; (2) L1/R1-transposition regular AG-groupoids are LR-transposition regular AG-groupoids, and an example is given to illustrate that not every LR-transposition regular AG-groupoid is an L1/R1-transposition regular AG-groupoid; (3) an AG-groupoid is an L1/R1-transposition regular AG-groupoid if it is an LR-transposition regular AG-groupoid satisfying a certain condition; (4) strong L2/R3-transposition regular AG-groupoids are equivalent to each other, and they are union of disjoint Abelian subgroups; (5) strong L3/R2-transposition regular AG-groupoids are equivalent to each other and they can be decomposed into union of disjoint AG subgroups. Their relations are discussed. Finally, we introduce various transposition regular AG-groupoid semigroups and discuss the relationships among them and the commutative Clifford semigroup as well as the Abelian group.