Legionella pneumophila Cas2 Promotes the Expression of Small Heat Shock Protein C2 That Is Required for Thermal Tolerance and Optimal Intracellular Infection

Campbell, Jackson; Cianciotto, Nicholas P.

doi:10.1128/iai.00369-22

Cited by 10 publications

(4 citation statements)

References 133 publications

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“…Normally, rhizobium genome encodes 4 degP proteins, which have been implicated in stress responses (Glazebrook et al ., 1996). hspC2, on the contrary, is a small heat shock protein known to be important for bacterial resistance to high temperature in bacteria (Campbell & Cianciotto, 2022). Interestingly, the expression of degP3 and hspC2 are probably controlled by the same operon in rhizobia (Narberhaus et al ., 1998).…”

Section: Resultsmentioning

confidence: 99%

NCR343 is required to maintain the viability of differentiated bacteroids in nodule cells in Medicago truncatula

et al. 2023

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Summary Bacteroid (name for rhizobia inside nodule cells) differentiation is a prerequisite for successful nitrogen‐fixing symbiosis. In certain legumes, under the regulation of host proteins, for example, a large group of NCR (nodule cysteine rich) peptides, bacteroids undergo irreversible terminal differentiation. This process causes them to lose the ability to propagate inside nodule cells while boosting their competency for nitrogen fixation. How host cells maintain the viability of differentiated bacteroids while maximizing their nitrogen‐reducing activities remains elusive. Here, through mutant screen, map‐based cloning, and genetic complementation, we find that NCR343 is required for the viability of differentiated bacteroids. In Medicago truncatula debino1 mutant, differentiated bacteroids decay prematurely, and NCR343 is proved to be the casual gene for debino1. NCR343 is mainly expressed in the nodule fixation zone, where bacteroids are differentiated. In nodule cells, mature NCR343 peptide is secreted into the symbiosomes. RNA‐Seq assay shows that many stress‐responsive genes are significantly induced in debino1 bacteroids. Additionally, a group of stress response‐related rhizobium proteins are identified as putative interacting partners of NCR343. In summary, our findings demonstrate that beyond promoting bacteroid differentiation, NCR peptides are also required in maintaining the viability of differentiated bacteroids.

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Section: Resultsmentioning

confidence: 99%

NCR343 is required to maintain the viability of differentiated bacteroids in nodule cells in Medicago truncatula

et al. 2023

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“…Additionally, they con rm que VapD has a cleavage site of the mRNA before adenine (A) and guanine (G), indicating that it has purine-speci c endoribonuclease activity [9]. On the other hand, studies done in Legionella pneumophila have showed that Cas2 play an important role in promotes the expression of several proteins, among them a small heat shock protein C2 (HspC2) required for thermal tolerance and the intracellular infection in Acanthamoeba castellanii, in uencing in the virulence and physiology of the bacterium [19]. Gunderson et al, determined that Cas2 protein is indispensable for Legionella pneumophila to successfully infect the amoebae and it has RNasa and DNasa activities, reinforcing the published for other authors, who demonstrated that the Cas proteins have other different role to phage/plasmid immunity [20], as it is known, the Cas2 protein participates in the stress response in Mycobacterium tuberculosis [21].…”

Section: Discussionmentioning

confidence: 99%

Expression of the VapD protein by Helicobacter pylori during intracellular infection

Flores-Alanis,

Delgado,

Santiago-Olivares

et al. 2024

Preprint

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Helicobacter pylori genotype I is strongly associated with different gastric pathologies and with the development of gastric cancer. There are several virulence factors that act in synergy to damage the gastric epithelium. Recently, the participation of the vapD gene in the persistence of Helicobacter pylori inside the gastric cell has been described. The objective of present study was to determine the VapD protein expression and location of Helicobacter pylori, when the microorganism is inside the eukaryote cell. Our research group had to development the recombinant VapD protein, polyclonal antibodies against VapD, cocultures of Helicobacter pylori and AGS cells and an immunofluorescence assay. Our results show that the VapD protein is expressed when the bacteria are inside the eukaryote cell. Probably maintaining homeostasis in the bacteria to resist the stress exerted by the intracellular environment of the gastric cell.

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“…All primers and plasmids used in this study are listed in Supplementary Data 3 and 4 , respectively. To make the L. pneumophila NU468 and NU469 mutant strains that have a nonpolar, unmarked deletion within the lcl gene, we employed overlap extension PCR (OE-PCR) followed by allelic exchange, as before 13 , 62 . DNA fragments of the 5′ and 3′ regions flanking the lcl ORF were PCR-amplified from 130b DNA using the primer pairs lcl -UpF and lcl -UpR for 5′ lcl , and lcl -DownF and lcl -DownR for 3′ lcl .…”

Section: Methodsmentioning

confidence: 99%

The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans

Rehman,

Antonovic,

McIntire

et al. 2024

Nat Commun

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Bacterial adhesion is a fundamental process which enables colonisation of niche environments and is key for infection. However, in Legionella pneumophila, the causative agent of Legionnaires’ disease, these processes are not well understood. The Legionella collagen-like protein (Lcl) is an extracellular peripheral membrane protein that recognises sulphated glycosaminoglycans on the surface of eukaryotic cells, but also stimulates bacterial aggregation in response to divalent cations. Here we report the crystal structure of the Lcl C-terminal domain (Lcl-CTD) and present a model for intact Lcl. Our data reveal that Lcl-CTD forms an unusual trimer arrangement with a positively charged external surface and negatively charged solvent exposed internal cavity. Through molecular dynamics simulations, we show how the glycosaminoglycan chondroitin-4-sulphate associates with the Lcl-CTD surface via distinct binding modes. Our findings show that Lcl homologs are present across both the Pseudomonadota and Fibrobacterota-Chlorobiota-Bacteroidota phyla and suggest that Lcl may represent a versatile carbohydrate-binding mechanism.

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Legionella pneumophila Cas2 Promotes the Expression of Small Heat Shock Protein C2 That Is Required for Thermal Tolerance and Optimal Intracellular Infection

Cited by 10 publications

References 133 publications

NCR343 is required to maintain the viability of differentiated bacteroids in nodule cells in Medicago truncatula

NCR343 is required to maintain the viability of differentiated bacteroids in nodule cells in Medicago truncatula

Expression of the VapD protein by Helicobacter pylori during intracellular infection

The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans

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