1996
DOI: 10.1128/iai.64.2.629-636.1996
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Legionella pneumophila mutants that are defective for iron acquisition and assimilation and intracellular infection

Abstract: Legionella pneumophila, a parasite of macrophages and protozoa, requires iron for optimal extracellular and intracellular growth. However, its mechanisms of iron acquisition remain uncharacterized. Using mini-Tn10 mutagenesis, we isolated 17 unique L. pneumophila strains which appeared to be defective for iron acquisition and assimilation. Eleven of these mutants were both sensitive to the iron chelator ethylenediamine di(ohydroxyphenylacetic acid) and resistant to streptonigrin, an antibiotic whose lethal eff… Show more

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Cited by 77 publications
(39 citation statements)
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“…While CcmC is sufficient to transfer haem onto CcmE, it is loaded in a very stable dead-end complex when CcmA or CcmB are absent. It can not be ruled out that CcmC may have additional roles that are independent of CcmAB in some organisms, such as pyoveridine production in Pseudomonas fluorescens (Mossialos et al, 2002), siderophore production in Paracoccus denitrificans (Page and Ferguson, 1999) and iron acquisition/ assimilation in Legionella pneumophilia (Pope et al, 1996;Viswanathan et al, 2002). CcmC may be constitutively produced for these functions, even when c-type cytochromes or a holoCcmE are not required.…”
Section: A Ccma 2b1c1 Release Function In System I Haem Deliverymentioning
confidence: 99%
“…While CcmC is sufficient to transfer haem onto CcmE, it is loaded in a very stable dead-end complex when CcmA or CcmB are absent. It can not be ruled out that CcmC may have additional roles that are independent of CcmAB in some organisms, such as pyoveridine production in Pseudomonas fluorescens (Mossialos et al, 2002), siderophore production in Paracoccus denitrificans (Page and Ferguson, 1999) and iron acquisition/ assimilation in Legionella pneumophilia (Pope et al, 1996;Viswanathan et al, 2002). CcmC may be constitutively produced for these functions, even when c-type cytochromes or a holoCcmE are not required.…”
Section: A Ccma 2b1c1 Release Function In System I Haem Deliverymentioning
confidence: 99%
“…Pope et a/. have recently reported the isolation of L. pneumophila mutants that are defective in iron acquisition and in intracellular infection (Pope et a/., 1996). The phenotype of the mutants did not manifest the dramatic killing at early stages of the infection compared to em/ mutants upon infection of macrophages and are rRNA, which would dramatically reduce the efficiency of obtaining DD-PCR fragments.…”
Section: Conservation Of Em1 Among Different Serogroups Of L Pneumopmentioning
confidence: 99%
“…These molecules have a high affinity for iron and sequester available free iron required for bacterial growth. Therefore, many bacterial pathogens, such as Yersinia, Salmonella and Legionella, require iron acquisition systems to survive the iron-limited environment of their host (Pope et al, 1996;Bearden and Perry, 1999;Janakiraman and Slauch, 2000). Recent results suggest an essential role for iron acquisition in Mycobacterium tuberculosis, the aetiological agent of tuberculosis, during infection.…”
Section: Introductionmentioning
confidence: 99%