Legionella pneumophila Philadelphia-1 tatB and tatC affect intracellular replication and biofilm formation

Buck, Emmy De; Maes, Liesbeth; Meyen, Eef; Mellaert, Lieve Van; Geukens, Nick; Anné, Jozef; Lammertyn, Elke

doi:10.1016/j.bbrc.2005.04.060

Cited by 69 publications

(68 citation statements)

References 24 publications

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“…Although the Tat pathway is important for biofilm formation, replication within the host, growth under iron-limiting conditions, and secretion of phospholipase C activity, no Tat substrate directly involved in virulence of L. pneumophila has been identified so far (33,108). The Tat pathway is also thought to be required for Helicobacter pylori and other gut bacteria to become established in their host organism (92).…”

Section: Role In Pathogenesismentioning

confidence: 99%

The Bacterial Twin-Arginine Translocation Pathway

Lee

Tullman‐Ercek

Georgiou

2006

Annu. Rev. Microbiol.

296

241

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The twin-arginine translocation (Tat) pathway is responsible for the export of folded proteins across the cytoplasmic membrane of bacteria. Substrates for the Tat pathway include redox enzymes requiring cofactor insertion in the cytoplasm, multimeric proteins that have to assemble into a complex prior to export, certain membrane proteins, and proteins whose folding is incompatible with Sec export. These proteins are involved in a diverse range of cellular activities including anaerobic metabolism, cell envelope biogenesis, metal acquisition and detoxification, and virulence. The Escherichia coli translocase consists of the TatA, TatB, and TatC proteins, but little is known about the precise sequence of events that leads to protein translocation, the energetic requirements, or the mechanism that prevents the export of misfolded proteins. Owing to the unique characteristics of the pathway, it holds promise for biotechnological applications.

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Section: Role In Pathogenesismentioning

confidence: 99%

The Bacterial Twin-Arginine Translocation Pathway

Lee

Tullman‐Ercek

Georgiou

2006

Annu. Rev. Microbiol.

296

241

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“…In L. pneumophila Philadelphia (De Buck et al, 2005) and in L. pneumophila strain 130b (Rossier & Cianciotto, 2005) the tatA, tatB and tatC genes were identified. In L. pneumophila Philadelphia the tat genes are expressed in all growth phases and also when cells grow intracellularly in the host Acanthamoeba castellanii.…”

Section: Protein Transport Across the Inner Membranementioning

confidence: 99%

“…Furthermore, it is important for stress response, motility and biofilm formation. Several methods were used to identify putative Tat substrates and proteins that are important for the motility and virulence of L. pneumophila, such as the virulence protein phospholipase C PlcA, which was shown to be Tat dependent (De Buck et al, 2005). Also an inner-membrane protein, the Rieske iron-sulfur protein PetA, was shown to be a Tat substrate.…”

Section: Protein Transport Across the Inner Membranementioning

confidence: 99%

The role of protein secretion systems in the virulence of the intracellular pathogen Legionella pneumophila

2007

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“…Mampel et al (2006) reported that biofilm formation by L. pneumophila in complex nutrient-rich media most likely relies on adhesion of bacteria grown in the planktonic phase. Also, the Tat secretion pathway has been shown to be involved in the biofilm formation ability of L. pneumophila (De Buck et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of Legionella pneumophila biofilm cells and the influence of iron on biofilm formation

et al. 2008

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In aquatic environments, biofilms constitute an ecological niche where Legionella pneumophila persists as sessile cells. However, very little information on the sessile mode of life of L. pneumophila is currently available. We report here the development of a model biofilm of L. pneumophila strain Lens and the first transcriptome analysis of L. pneumophila biofilm cells. Global gene expression analysis of sessile cells as compared to two distinct populations of planktonic cells revealed that a substantial proportion of L. pneumophila genes is differentially expressed, as 2.3 % of the 2932 predicted genes exhibited at least a twofold change in gene expression. Comparison with previous results defining the gene expression profile of replicative-and transmissive-phase Legionella suggests that sessile cells resemble bacteria in the replicative phase. Further analysis of the most strongly regulated genes in sessile cells identified two induced gene clusters. One contains genes that encode alkyl hydroperoxide reductases known to act against oxidative stress. The second encodes proteins similar to PvcA and PvcB that are involved in siderophore biosynthesis in Pseudomonas aeruginosa. Since iron has been reported to modify biofilm formation in other species, we further focused on iron control of gene expression and biofilm formation. Among the genes showing the greatest differences in expression between planktonic cells and biofilm, only pvcA and pvcB were regulated by iron concentration. A DpvcA L. pneumophila mutant showed no changes in biofilm formation compared to the wild-type, suggesting that the pvcA product is not mandatory for biofilm formation. However, biofilm formation by L. pneumophila wild-type and a DpvcA strain was clearly inhibited in iron-rich conditions.

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Legionella pneumophila Philadelphia-1 tatB and tatC affect intracellular replication and biofilm formation

Cited by 69 publications

References 24 publications

The Bacterial Twin-Arginine Translocation Pathway

The Bacterial Twin-Arginine Translocation Pathway

The role of protein secretion systems in the virulence of the intracellular pathogen Legionella pneumophila

Transcriptional profiling of Legionella pneumophila biofilm cells and the influence of iron on biofilm formation

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