Legionella pneumophila sg1-sensing signal enhancement using a novel electrochemical immunosensor in dynamic detection mode

Laribi, Ahlem; Allegra, Séverine; Souiri, Mina; Mzoughi, Ridha; Othmane, Ali; Girardot, Françoise

doi:10.1016/j.talanta.2020.120904

Cited by 11 publications

(4 citation statements)

References 47 publications

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“…For instance, Park, et al [ 10 ] have reported a DNA biosensor for the specific detection of L. pneumophila , but the extraction of DNA from bacteria is associated with a number of processing steps, resulting in laborious and costly analysis. Whole cell L. pneumophila biosensors have frequently been investigated based on electrochemical impedance spectroscopy (EIS) [ 11 , 12 ], surface plasmon resonance (SPR) [ 13 ], and colorimetric detection [ 14 , 15 ], as presented in Table 1 . EIS biosensors have received significant attention due to their sensitivity and cost effectiveness [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy

et al. 2022

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Rapid detection of Legionella pneumophila (L. pneumophila) is important for monitoring the presence of these bacteria in water sources and preventing the transmission of the Legionnaires’ disease. We report improved biosensing of L. pneumophila with a digital photocorrosion (DIP) biosensor functionalized with an innovative structure of cysteine-modified warnericin antimicrobial peptides for capturing bacteria that are subsequently decorated with anti-L. pneumophila polyclonal antibodies (pAbs). The application of peptides for the operation of a biosensing device was enabled by the higher bacterial-capture efficiency of peptides compared to other traditional ligands, such as those based on antibodies or aptamers. At the same time, the significantly stronger affinity of pAbs decorating the L. pneumophila serogroup-1 (SG-1) compared to serogroup-5 (SG-5) allowed for the selective detection of L. pneumophila SG-1 at 50 CFU/mL. The results suggest that the attractive sensitivity of the investigated sandwich method is related to the flow of an extra electric charge between the pAb and a charge-sensing DIP biosensor. The method has the potential to offer highly specific and sensitive detection of L. pneumophila as well as other pathogenic bacteria and viruses.

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Section: Introductionmentioning

confidence: 99%

Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy

et al. 2022

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“…In that context, various types of L. pneumophila biosensors have been investigated ranging from optical , and piezoelectric , to electrochemical. , However, to the best of our knowledge, an economically attractive method for automated monitoring of water reservoirs for the presence of pathogenic bacteria has yet to be developed. Recently, photoluminescence (PL)-based detection of E.…”

Section: Introductionmentioning

confidence: 99%

Short Ligand, Cysteine-Modified Warnericin RK Antimicrobial Peptides Favor Highly Sensitive Detection of Legionella pneumophila

et al. 2021

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Culture-based methods for the detection of Legionella pneumophila are prohibitively slow and frequently inadequate. The problem has been addressed with biosensing technology that employs a variety of ligands for the specific capture of bacteria. However, the limited success of the application of mammalian antibodies, aptamers, and nucleic acid-based probes for sensitive biosensing has generated growing interest in exploring alternative biosensing architectures, such as those based on antimicrobial peptides (AMP) that are known for their attractive therapeutic applications. We report on the successful employment of cysteinemodified warnericin RK AMP for the operation of a highly sensitive biosensor of L. pneumophila based on digital photocorrosion of GaAs/AlGaAs nanoheterostructures. The replacement of the relatively cumbersome procedure commonly applied for the attachment of antibodies to COOH-terminated mercaptohexadecanoic acid self-assembled monolayers has allowed for a significant reduction in the distance at which bacteria are immobilized above the biosensor surface. An important consequence of this approach is the attractive limit of detection of L. pneumophila estimated at 2 × 10 2 CFU/mL. The target bacteria were captured four times more efficiently than P. fluorescens, B. subtilis, and E. coli, which is highly promising for environmental monitoring.

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“…(21-mer DNA sequence) Differential pulse voltammetry Approx 45 min (PCR NOT INCLUDED) YES Asymmetric PCR mismatched DNA 3.1 × 10 −13 M of synthetic DNA coding for 21-mer gene [ 30 ] Electrochemical genosensor based on amplified molecular beacon for the detection of Legionella spp. (21-mer DNA sequence) using thiolated hairpin DNA-ferrocene probes on gold electrodes Differential pulse voltammetry 45 min approx (PCR NOT INCLUDED) YES PCR Mismatched DNA 2.3 × 10 −14 M of synthetic DNA coding for 21-mer gene [ 31 ] Microfluidic electrochemical genosensor for the detection of target sequence ( L. pneumophila ) based on two-step sandwich with strep-coated paramagnetic microparticles, a biotinylated capture probe and strep-AP (streptavidin-alkaline phosphatase conjugate) Amperometry 16 min (PCR NOT INCLUDED) NO Mismatched DNA 0.33 nM of synthetic DNA [ 32 ] Electrochemical genosensor for the detection of the target sequence ( mip gene sequence) of L. pneumophila based on a multiwall carbon nanotube (MWCNT) electrode Differential pulse voltammetry 40 min (PCR NOT INCLUDED) NO Mismatched DNA 10 pM of synthetic DNA coding for mip gene [ 33 ] Electrochemical immunosensor for the detection of L. pneumophila developing an immunoassay in nitrocellulose filters Amperometry 2–3 h YES Filtration of 200 mL (The antibody used in this paper was previously tested in another work) 4 CFU mL −1 [ 34 ] Electrochemical immunosensor based on a microfluidic assay in water samples for the detection of L. pneumophila Square wave voltammetry 100–180 min (depending on the detection mode, dynamic or static) NO NO 10 CFU mL −1 [ 20 ] Electrochemical magneto immunosensor for the detection of L. pneumophila based on disposable core-shell Fe 3 O 4 @poly(dopamine) magnetic nanoparticles and a sandwich enzyme-linked i...…”

Section: Resultsmentioning

confidence: 99%

Improved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters

Mesas Gómez,

Molina-Moya,

de Araujo Souza

et al. 2024

Microchim Acta

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A novel approach is presented that combines filtration and the direct immunomagnetic separation of the retained bacteria Legionella in filters, for further electrochemical immunosensing. This strategy allows for the separation and preconcentration of the water-borne pathogen from high-volume samples, up to 1000 mL. The limit of detection of the electrochemical immunosensor resulted in 100 CFU mL−1 and improved up to 0.1 CFU mL−1 when the preconcentration strategy was applied in 1 L of sample (103-fold improvement). Remarkably, the immunosensor achieves the limit of detection in less than 2.5 h and simplified the analytical procedure. This represents the lowest concentration reported to date for electrochemical immunosensing of Legionella cells without the need for pre-enrichment or DNA amplification. Furthermore, the study successfully demonstrates the extraction of bacteria retained on different filtering materials using immunomagnetic separation, highlighting the high efficiency of the magnetic particles to pull out the bacteria directly from solid materials. This promising feature expands the applicability of the method beyond water systems for detecting bacteria retained in air filters of air conditioning units by directly performing the immunomagnetic separation in the filters. Graphical abstract

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Legionella pneumophila sg1-sensing signal enhancement using a novel electrochemical immunosensor in dynamic detection mode

Cited by 11 publications

References 47 publications

Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy

Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy

Short Ligand, Cysteine-Modified Warnericin RK Antimicrobial Peptides Favor Highly Sensitive Detection of Legionella pneumophila

Improved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters

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