Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis

Characterization of functional properties of proteins from Ganxet beans (Phaseolus vulgaris L. var. Ganxet) isolated using an ultrasound-assisted methodology

“…Finally, very weak bands corresponding to molecular sizes of 12.03 and 9.89 kDa were also obtained. The protein profile observed M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 13 was highly similar to those reported by Mirali, El-Khouri, and Rizq (2007) and Nikolić, Đorđević, Torbica, and Mikić (2012), which were found to be highly dependent on the variety of Vicia faba employed. These same authors reported how the polymorphism present in Vicia species could alter the profile of the extracted proteins.…”

Nutritive quality and protein production from grain legumes in a boreal climate

“…Globulins account for about 50–90% of the storage protein in most grain legumes and are generally divided into the 7S vicilins (conglutin β in lupins) and 11S legumins (conglutin α in lupins). Conglutin γ of lupins, a basic 7S protein, is of interest because of its high concentration of the sulfur‐containing amino acids cysteine and methionine, and, in our experiments, narrow‐leafed lupin was the species with the highest concentration of these two amino acids.…”

Processing and sensory characteristics of a fermented low‐fat skim milk drink containing bioactive antihypertensive peptides, a functional milk product

“…The preparation of dye and samples on a chip was carried out according to the manufacturer's recommendation and as previously described, with minor modifications (Nikolić et al . ). Briefly, 0.5 μL of reconstituted dye solution was added to 5 μL of protein ladder (5–240 kD) and 5 μL of sample in microtubes, respectively, then vortexed and incubated for 30 min on ice.…”