Leishman and <scp>G</scp>iemsa stain: a new reliable staining technique for blood/bone marrow smears

Gajendra, Smeeta; Jha, Bhawna; Goel, Shalini; Sahni, Tushar; Sharma, Ridhima; Shariq, M.; Jaiswal, Shradha; Sachdev, Ritesh

doi:10.1111/ijlh.12408

Cited by 21 publications

(14 citation statements)

References 4 publications

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“…The number of megakaryocytes in a 1.5×3.5-cm area of a standard bone marrow smear were determined under microscope (BX53; Olympus Corporation, Tokyo, Japan) using Wright's staining (Sigma-Aldrich, St. Louis, MO, USA) in all patients and donors, as described previously ( 17 , 18 ). Megakaryocyte morphology was also analyzed in all patients and donors ( 17 , 18 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes

Ding

Nachnani

et al. 2016

Oncology Letters

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Thrombocytopenia is a common, often fatal complication experienced by patients with myelodysplastic syndromes (MDS). 5-aza-2′-deoxycytidine (decitabine) has been used to treat MDS patients with thrombocytopenia with a response rate of 45–50%. However, the mechanism of its effects on megakaryocytes remains unclear. In the present study, the effect of decitabine on megakaryocyte maturation was investigated. A total of 20 MDS patients diagnosed with thrombocytopenia were enrolled, including 16 refractory anemia with excess blasts (RAEB)-1 patients and 4 RAEB-2 patients], in addition to 20 leukemia patients that had achieved complete remission and 20 healthy donors. Overall, 65% of MDS patients exhibited a response to decitabine, with an increase in platelet count identified in 80% of patients. In the MDS group, the mean platelet count was significantly increased following one cycle of decitabine chemotherapy (36.85±24.54 vs. 84.90±61; P=0.001); however, no significant difference in megakaryocyte number was identified prior to and following treatment. Additionally, bone marrow mononuclear cells of the MDS patients were cultured in vitro with various concentrations of decitabine (0.0, 2.0, 2.5, 3.0 µM), and cluster of differentiation (CD)41 levels were examined via flow cytometry. The MDS and normal control groups exhibited the highest levels of CD41 expression following treatment with 2.0 µM decitabine (mean fluorescence intensity, 294.07±47.34 and 258.95±28.05, respectively). In conclusion, these results indicate that the DNA-hypomethylating agent, decitabine, may induce the differentiation and maturation of myelodysplastic megakaryocytes in MDS patients, even at low concentrations. Thus, the repeated administration of decitabine at lower doses in MDS patients may be useful in clinical practice, and may lead to the development of alternative treatments for other diseases of abnormal megakaryocyte differentiation, such as idiopathic thrombocytopenic purpura, however, future studies are required to investigate this.

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Section: Methodsmentioning

confidence: 99%

Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes

Ding

Nachnani

et al. 2016

Oncology Letters

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“…Extensive studies based on the cytoplasmic and nuclear characteristics associated with periodontitis have not been done elsewhere. Gjendra et al 27 reported that LG combination gives better nuclear morphology, crisp nuclear and cytoplasmic contrast, staining of cytoplasmic granules, and metachromasia to the background material in hematological samples. These staining characteristics of LG cocktail stain were utilized in this study for the assessment and grading of the inflammatory features of the epithelial cells associated with periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Cellular composition and cytomorphological characteristics of periodontal pockets of patients with periodontitis using Leishman‐Giemsa cocktail staining

Kavya

Abraham

Padmakumar

et al. 2020

Diagnostic Cytopathology

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“…Well-stained blood film is very important for right cell structure assesment. There are some tried staining techniques like Leishman and Giemsa to dye various smears [14]. Authors' work is upon trying of a plant dye in blood cells.…”

Section: Discussionmentioning

confidence: 99%

Staining Effect of Pomegranate Flower Extract on Human Blood Cells: First Results

Kuşculuo¹,

Benli²

2017

JESE-A

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Pomegranate flower plant, popularly known using for the treatment of various diseases, was not investigated as a source of dye for cytological studies using human blood cells. The importance of this study is to appear dyeing result of pomegranate flower extract on human blood cells. The natural dye source was pomegranate flower known as roselle and potassium aluminum sulfate (alum = KAlSO 4 12H 2 O) was used as mordant or metal salt. Distilled water was used as solvent. Fresh, clean and air-dried flowers were extracted with distilled water at 100 °C for 30 minutes and then filtered. One drop blood from a healthy 20-year woman was spread as a peripheral on to ten plates and dried at nearly 25 °C. These slides were stained by soaking in pomegranate flower extract with/without alum (KAlSO 4 12H 2 O) at 100 °C for 60 minutes. Slides were washed with distilled water, dried and done microscobic examination. The different blood cells dyed dark orange in alum mordant media at 100 °C. As a result, pomegranate flower has the capacity to use dyeing human blood cells such as eosinophil, basophil and neutrophil.

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Leishman and Giemsa stain: a new reliable staining technique for blood/bone marrow smears

Cited by 21 publications

References 4 publications

Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes

Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes

Cellular composition and cytomorphological characteristics of periodontal pockets of patients with periodontitis using Leishman‐Giemsa cocktail staining

Staining Effect of Pomegranate Flower Extract on Human Blood Cells: First Results

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