Leishmania donovani: Thionins, plant antimicrobial peptides with leishmanicidal activity

Berrocal-Lobo, Marta; Molina, Antonio; Rodrı́guez-Palenzuela, Pablo; Garcı́a-Olmedo, Francisco; Rivas, Luís

doi:10.1016/j.exppara.2009.03.019

Cited by 50 publications

(36 citation statements)

References 12 publications

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“…We observed that at 300 μg/ml, PvD 1 was able to inhibit 70 % of the protozoan proliferation, after 24 h of incubation (Figure 1). Berrocal-Lobo et al [30] observed the activity of a PTH1 defensin from Solanum tuberosum and a thionin from Triticum aestivum against L. donovani; however, these authors did not test these peptides against L. amazonensis. More recently, Souza et al [29] showed that the natural defensin from Vigna unguiculata seeds (Vu-Def), as well as its recombinant homologues (Vu-Defr), both .…”

Section: Discussionmentioning

confidence: 99%

PvD1 defensin, a plant antimicrobial peptide with inhibitory activity against Leishmania amazonensis

et al. 2015

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SynopsisPlant defensins are small cysteine-rich peptides and exhibit antimicrobial activity against a variety of both plant and human pathogens. Despite the broad inhibitory activity that plant defensins exhibit against different micro-organisms, little is known about their activity against protozoa. In a previous study, we isolated a plant defensin named PvD 1 from Phaseolus vulgaris (cv. Pérola) seeds, which was seen to be deleterious against different yeast cells and filamentous fungi. It exerted its effects by causing an increase in the endogenous production of ROS (reactive oxygen species) and NO (nitric oxide), plasma membrane permeabilization and the inhibition of medium acidification. In the present study, we investigated whether PvD 1 could act against the protozoan Leishmania amazonensis. Our results show that, besides inhibiting the proliferation of L. amazonensis promastigotes, the PvD 1 defensin was able to cause cytoplasmic fragmentation, formation of multiple cytoplasmic vacuoles and membrane permeabilization in the cells of this organism. Furthermore, we show, for the first time, that PvD 1 defensin was located within the L. amazonensis cells, suggesting the existence of a possible intracellular target.

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Section: Discussionmentioning

confidence: 99%

PvD1 defensin, a plant antimicrobial peptide with inhibitory activity against Leishmania amazonensis

et al. 2015

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“…In vitro thionins are toxic at micromolar concentrations to a wide range of organisms from bacteria and fungi to protozoa and human cells (Fujimura et al 2005;Loeza-Ángeles et al 2008;Berrocal-Lobo et al 2009). Thionins have been found in both seeds and vegetative organs, such as leaves and stems (Stec 2006).…”

Section: Thioninsmentioning

confidence: 99%

New Antimicrobial Agents of Plant Origin

Sampedro

Valdivia

2013

Antimicrobial Compounds

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Plants are constantly under attack by microbial pathogens. As part of their defensive arsenal, they use antimicrobial peptides such as thionins, defensins, lipid transfer proteins, hevein-like peptides, knottins, cyclotides, b-barrelins, and others. In addition, they produce a diversity of antimicrobial metabolites. Those where the evidence for a role in plant defense is stronger include benzoxazinoids, camalexin, and glucosinolates among the alkaloids; flavonoids and stilbenes among the phenylpropanoids; and also terpenoids such as saponins. Our understanding of these plant antimicrobial agents has increased significantly in recent years with new information on their distribution, synthesis, regulation, in vivo function, and mechanism of action. Plant antimicrobial agents have a large potential for biotechnological applications. Engineered plants with increased disease resistance have been achieved using almost every family of antimicrobial peptides. There have been also been successes in using metabolic engineering to increase the production of antimicrobial compounds, as in the case of stilbenes and glucosinolates. Commercial applications using both approaches are likely to appear soon. The use of plant antimicrobials in human medicine is probably further in the future, although there are promising antifungal agents like defensin peptides, and saponins. With more than a quarter million species and a particularly diverse specialized metabolism, the richness of plant antimicrobials has barely been explored. Plant Antimicrobial DefensesWild plants are quite successful at keeping bacterial and fungal pathogens at bay. In addition to physical barriers, they have an immune system capable of detecting and responding to the presence of pathogens. The first layer of defense is based on

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“…Other researchers [49] have also discovered new anti-malarial peptides isolated from the non-coding regions of the yeast genome. Berrocal-Lobo et al [7] described in vitro activity of the plant antimicrobial peptides thionins against Leishmania donovani.…”

Section: The Application Of Peptides In Ophthalmologymentioning

confidence: 99%

“…AMPs include all oligoand polypeptides that kill microbes or inhibit their growth, peptides derived from larger proteins, and non-ribosomally synthesized examples [3]. AMPs possess various mechanisms of action, such as permeabilisation of microbial membranes, destabilisation of bilayer lipid structures, formation of micelles or channels within a membrane, lipopolysaccharide (LPS) binding, inhibition of DNA replication and expression of proteins or release of ATP [2,3,4,5,6,7,8,9]. A detailed review of research on peptides can be found in the work of Hancock & Lehrer [4], Zasloff [10], Brodgen et al [11], Mirski et al [12], Kołodziej et al [13], Eckert [14], Brandenburg et al [15], and Devocelle [16].…”

Section: Introductionmentioning

confidence: 99%

Utilisation of peptides against microbial infections – a review

Mirski

Niemcewicz

Bartoszcze

et al. 2017

Ann Agric Environ Med.

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The emergence of resistance in microorganisms on a global scale has made it necessary to search for new antimicrobial factors. Antimicrobial peptides (AMPs) seem to meet these expectations. AMPs are produced by bacteria, viruses, plants, and animals, and may be considered as a new class of drugs intended for the prophylaxis and treatment of both systemic and topical infections. The aim of this study is to review the results of studies on the use of peptides to combat infections in vivo. Antimicrobial peptides may be applied topically and systemically. Among the peptides used topically, a very important area for their application is ophthalmology. AMPs in ophthalmology may be used mainly for the protection of contact lenses from ocular pathogens. Many AMPs are in clinical trials for application in the therapy of local infections. There may be mentioned such preparations as: pexiganan (magainin analogue), MX-226 (based on indolicidin), NEUPREX (isolated from human BPI (bactericidal/permeability-increasing) protein), IB-367 (variant of porcine protegrin), P113 (based on histatin), daptomycin, polymyxins, as well as peptidomimetics. In the combat against systemic infections are used such peptides as: P113D (modified P113 peptide containing D-amino acids), colistin, peptoids, and peptides containing non-typical amino acids or non-peptide elements. AMPs are also used as antiprotozoal, antifungal, antitoxic and immunostimulatory agents. The limitations in the use of peptides in the treatment of infections, such as susceptibility to proteolysis, and resistance of microorganisms to the peptides, are also discussed. AMPs are a promising strategy in the fight against microbial infections.

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Leishmania donovani: Thionins, plant antimicrobial peptides with leishmanicidal activity

Cited by 50 publications

References 12 publications

PvD1 defensin, a plant antimicrobial peptide with inhibitory activity against Leishmania amazonensis

PvD1 defensin, a plant antimicrobial peptide with inhibitory activity against Leishmania amazonensis

New Antimicrobial Agents of Plant Origin

Utilisation of peptides against microbial infections – a review

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