Leishmania infantumPromastigotes: Flow Cytometry as a Possible Tool for Assessing the Effects of Drugs on Cellular Functions

Azas, Nadine; Giorgio, Carole Di; Delmas, F.; Gasquet, M.; Timon‐David, P.

doi:10.1006/expr.1997.4182

Cited by 25 publications

(17 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results confirmed that, contrary to pentamidine which has been shown to inhibit intracellular metabolism such as DNA synthesis and polyamine metabolism (Azas et al, 1997), amphotericin B and saponins could react rapidly with Leishmania membrane by inducing, immediately after treatment, a strong decrease of membrane integrity (Danloy et al, 1994). Moreover, they demonstrated that the impact of saponins on parasite membrane could cumulate with those of the polyene antibiotic.…”

Section: Antileishmanial Activity Of Ivy 299supporting

confidence: 69%

See 1 more Smart Citation

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A₁, in association with pentamidine and amphotericin B

Ridoux

Giorgio

Delmas

et al. 2001

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

The in vitro antileishmanial activity of three saponins isolated from ivy, alpha-hederin, beta-hederin and hederacolchiside A(1), was investigated on parasites of the species Leishmania mexicana, in their promastigote and amastigote forms compared with their toxicity versus human monocytes. The results showed that saponins exhibited a strong antiproliferative activity on all stages of development of the parasite but demonstrated a strong toxicity versus human cells. Association of subtoxic concentrations of saponins with antileishmanial drugs such as pentamidine and amphotericin B demonstrated that saponins could enhance the efficiency of conventional drugs on both the promastigote and the amastigote stages of development of the parasite. The results demonstrated moreover that the action of saponins on promastigote membrane was cumulative with those of amphotericin B.

show abstract

Section: Antileishmanial Activity Of Ivy 299supporting

confidence: 69%

“…The impact of drugs on the membrane of Leishmania mexicana was assessed according to the methodology previously described by Azas et al (1997) using improved flow cytometric techniques. Promastigotes in late log phase were incubated in RPMI medium supplemented with 12% fetal calf serum, at an average of 10 5 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A₁, in association with pentamidine and amphotericin B

Ridoux

Giorgio

Delmas

et al. 2001

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Saul et al (1982) successfully used PI to detect and count Plasmodium falciparum infected cells by flow cytometry. With this technique Azas et al (1997), studied the effect of pentamidine, allopurinol, and amphotericin B on cell growth and in the parasite metabolism. Cell multiplication was studied by these authors by a dynamic analysis of DNA stained with propidium iodide.…”

Section: Discussionmentioning

confidence: 99%

Leishmanicidal Activity of Edelfosine, Miltefosine and Ilmofosine

Azzouz¹,

Maache²,

Garcia³

et al. 2005

Basic Clin Pharma Tox

View full text Add to dashboard Cite

Abstract:The anti-proliferative action of three alkyl-lysophospholipid derivatives, edelfosine (ET-OCH), miltefosine (Hexadecylphosphocholine), and ilmofosine (BM 14.440) has been studied on the promastigotes and amastigotes of Leishmania donovani. The effect of the three drugs has previously been studied, but the action mode was not clearly elucidated. In this study the effect on the intracellular amastigote forms was evaluated by two different methods: the traditional method, counting the amastigotes within the macrophages stained with Giemsa; and by a new method, staining the nuclear macrophages and amastigotes with ethidium bromide and counting the different population by flow cytometry. This new method, based on the flow cytometry, shows an advantage for evaluating the anti-proliferative effects in intracellular parasites. The ED50 were calculated for the drug activity after 72 hr, and for the three alkyl-lysophospholipid derivatives it were in the range of 26.73-33.31 mM against promastigotes and in the range of 16.46-23.16 against amastigotes. Also, studying the effect against macrophages J774A1, the ED50 were in the range of 24.28-26.38 mM. The effect of the alkyl-lysophospholipids in the macromolecular biosynthesis of the Leishmania donovani, was studied comparing the incorporation of labelled analogues ([

show abstract

“…A viability control free of drug and a control containing DMSO (final concentration of 0.5%) were performed in parallel. After a 72 h-incubation at 37 C and 6% CO 2 in complete RPMI medium, cell growth and viability were measured by flow cytometry after staining the monocytes by 5 mL of propidium iodide (1 mg/mL) (Sigma) [22].…”

Section: Cells Toxicity Assaysmentioning

confidence: 99%

Lowering of 5-nitroimidazole's mutagenicity: Towards optimal antiparasitic pharmacophore

Crozet

Botta²,

Gasquet³

et al. 2009

European Journal of Medicinal Chemistry

View full text Add to dashboard Cite

Leishmania infantumPromastigotes: Flow Cytometry as a Possible Tool for Assessing the Effects of Drugs on Cellular Functions

Cited by 25 publications

References 14 publications

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A₁, in association with pentamidine and amphotericin B

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A₁, in association with pentamidine and amphotericin B

Leishmanicidal Activity of Edelfosine, Miltefosine and Ilmofosine

Lowering of 5-nitroimidazole's mutagenicity: Towards optimal antiparasitic pharmacophore

Contact Info

Product

Resources

About

Leishmania infantumPromastigotes: Flow Cytometry as a Possible Tool for Assessing the Effects of Drugs on Cellular Functions

Cited by 25 publications

References 14 publications

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A1, in association with pentamidine and amphotericin B

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A1, in association with pentamidine and amphotericin B

Leishmanicidal Activity of Edelfosine, Miltefosine and Ilmofosine

Lowering of 5-nitroimidazole's mutagenicity: Towards optimal antiparasitic pharmacophore

Contact Info

Product

Resources

About

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A₁, in association with pentamidine and amphotericin B

In vitro antileishmanial activity of three saponins isolated from ivy, α‐hederin, β‐hederin and hederacolchiside A₁, in association with pentamidine and amphotericin B