Leishmania mexicana mexicana: Genetic Heterogeneity of Mexican Isolates Revealed by Restriction Length Polymorphism Analysis of Kinetoplast DNA

Berzunza-Cruz, Miriam; Bricaire, Guadalupe; Romero, Sierra; Perez-Becker, Ruy; Saavedra, Emma; Pérez‐Montfort, Ruy; Crippa-Rossi, M.; Velasco-Castrejón, Oscar; Becker, Ingeborg

doi:10.1006/expr.2000.4541

Cited by 15 publications

(8 citation statements)

References 13 publications

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“…Vector species with medical importance have shown to have the ability to establish populations in transformed areas after human interventions. 77 Our results showed a high proportion of potential distributions of species overlapping with transformed habitats, suggesting ample potential for domiciliation of the sand flies by shifting from a sylvatic to a peridomestic cycle. Clearly, landscape analyses along ecological gradients should be conducted to establish population dynamics of vectors and their impacts on parasite incidence and persistence.…”

Section: Discussionmentioning

confidence: 58%

Current Knowledge of Leishmania Vectors in Mexico: How Geographic Distributions of Species Relate to Transmission Areas

González¹,

Rebollar-Téllez²,

Ibáñez-Bernal³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Leishmaniases are a group of vector-borne diseases with different clinical manifestations caused by parasites transmitted by sand fly vectors. In Mexico, the sand fly Lutzomyia olmeca olmeca is the only vector proven to transmit the parasite Leishmania mexicana to humans, which causes leishmaniasis. Other vector species with potential medical importance have been obtained, but their geographic distributions and relation to transmission areas have never been assessed. We modeled the ecological niches of nine sand fly species and projected niches to estimate potential distributions by using known occurrences, environmental coverages, and the algorithms GARP and Maxent. All vector species were distributed in areas with known recurrent transmission, except for Lu. diabolica, which appeared to be related only to areas of occasional transmission in northern Mexico. The distribution of Lu. o. olmeca does not overlap with all reported cutaneous leishmaniasis cases, suggesting that Lu. cruciata and Lu. shannoni are likely also involved as primary vectors in those areas. Our study provides useful information of potential risk areas of leishmaniasis transmission in Mexico.

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Section: Discussionmentioning

confidence: 58%

Current Knowledge of Leishmania Vectors in Mexico: How Geographic Distributions of Species Relate to Transmission Areas

González¹,

Rebollar-Téllez²,

Ibáñez-Bernal³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…In previous reports, genetic polymorphism could be correlated with the geographical origin of parasite strains [12,27-29]or clinical phenotype [30-32]. Recently, we have demonstrated that L. braziliensis isolates differing at the genome level also show distinctive infection patterns in BALB/c mice [33].…”

Section: Discussionmentioning

confidence: 98%

Genetic diversity of Leishmania amazonensisstrains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping

Oliveira

Fernandes

Cruz

et al. 2007

Kinetoplastid Biol Dis

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Background: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement. In the present study, we have investigated the genetic variability of 18 L. amazonensis strains isolated in northeastern Brazil from patients with different clinical manifestations of leishmaniasis. Parasite DNA was analyzed by sequencing of the ITS flanking the 5.8 S subunit of the ribosomal RNA genes, by RAPD and SSR-PCR and by PFGE followed by hybridization with gene-specific probes.

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“…These results clearly suggest that the kDNA minicircles of the Mexican isolates are more polymorphic than the kDNA of the other members of the L. mexicana complex and have different recognition sites for the several restriction enzymes used in this study. Furthermore, in studies of PRC-RFLP of kDNA [16] found six basic digestion patterns in Mexican isolates of L. (L.) mexicana that were different from the digestion patterns displayed for the other members of the L. mexicana complex reference strains. …”

Section: Discussionmentioning

confidence: 99%

Analysis of Kinetoplast DNA from Mexican Isolates ofLeishmania (L.) mexicana

Hernandez-Montes

Guzmán²,

Gómez³

et al. 2012

Interdisciplinary Perspectives on Infectious Diseases

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This study analyzed DNA minicircles of Mexican isolates of L. (Leishmania) mexicana to look for genetic differences between strains isolated from patients with diffuse cutaneous (DCL) and localized (LCL) leishmaniasis. The kDNA was analyzed using polymerase chain reaction (PCR), restriction fragment polymorphism analysis of the PCR products (PCR-RFLP) and the PCR products were sequenced. In the PCR with primers specific for the subgenus Leishmania, the Mexican isolates gave higher amplification products than the other L. mexicana complex strains and with specific primers for the L. mexicana complex they were poorly amplified. In the PCR-RFLP analysis with the Eco RV, Hae III, and Mbo I endonucleases, the Mexican isolates displayed similar restriction patterns, but different from the patterns of the other members of the L. mexicana complex. In the phylogenetic tree constructed, the kDNA sequences of the Mexican clones formed two groups including sequences of LCD or LCL clones, apart from the other L. mexicana complex members. These results suggest that the kDNA minicircles of the Mexican isolates are more polymorphic than the kDNA of other members of the L. mexicana complex and have different recognition sites for the restriction enzymes used in this study.

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Leishmania mexicana mexicana: Genetic Heterogeneity of Mexican Isolates Revealed by Restriction Length Polymorphism Analysis of Kinetoplast DNA

Cited by 15 publications

References 13 publications

Current Knowledge of Leishmania Vectors in Mexico: How Geographic Distributions of Species Relate to Transmission Areas

Current Knowledge of Leishmania Vectors in Mexico: How Geographic Distributions of Species Relate to Transmission Areas

Genetic diversity of Leishmania amazonensisstrains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping

Analysis of Kinetoplast DNA from Mexican Isolates ofLeishmania (L.) mexicana

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