Leishmaniasis Diagnosed from Bronchoalveolar Lavage

Jokipii, Liisa; Salmela, K.; Saha, Heikki; Kyrönseppä, Hannu; Eklund, B; Evans, David A.; Willebrand, E von; Jokipii, A. M. M.

doi:10.3109/00365549209054657

Cited by 33 publications

(31 citation statements)

References 14 publications

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“…In humans there are reports of a few infected cells and amastigotes in interstitial tissue, alveolar septa and bronchoalveolar lavage (MELENEY, 1925;ANDRADE, 1959;JOKIPII et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Presence of amastigotes in the central nervous system of hamsters infected with Leishmania sp.

Oliveira

Oshiro

Pinto

et al. 2011

Rev. Bras. Parasitol. Vet.

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Visceral leishmaniasis (VL) is a severe chronic disease caused by Leishmania (Leishmania) infantum chagasi. Better knowledge on the effects caused by this disease can help develop adequate clinical management and treatment. Parasitological and immunohistochemical studies were performed golden hamsters Mesocricetus auratus infected with bone marrow from individuals with VL in the State of Mato Grosso do Sul, central-west Brazil. The effects of parasitism in the spleen, liver, kidneys, lungs, heart and brain of the animals were examined. Eighteen hamsters were inoculated intraperitoneally, and six healthy animals were used as negative controls. The animals were kept in the animal house and checked for clinical signs. Specimens of each organ were examined for the presence of amastigotes. Immunohistochemical technique was performed in all brain specimens and organs negative on the direct examination of parasites. Direct examination of amastigotes was positive in the spleen and liver of all infected animals; 33.3% showed the parasite in the kidneys and lungs, and 16.7% in the heart. Parasitic forms were seen in 83.3% (15/18) of the brain examined. Immunohistochemistry confirmed the results of the direct examination, except in two specimens of lung tissue and in the brain specimens. Other studies are needed to further clarify the effect of the parasite in the central nervous system. Keywords: Visceral leishmaniasis, immunohistochemistry, brain, hamster. ResumoA leishmaniose visceral (LV) é uma doença crônica grave, causada pelo parasito Leishmania (Leishmania) infantum chagasi. Esclarecer as alterações provocadas pela doença é fundamental para que se adotem condutas clínicas e de tratamento adequadas. Com o objetivo de analisar a infecção experimental em hamsters da linhagem golden, Mesocricetus auratus, infectados com tecido de medula óssea de pacientes com LV no Estado de Mato Grosso do Sul, foram realizados estudos parasitológicos e de imunomarcação. Foi verificada a distribuição do parasitismo no baço, fígado, rim, pulmão, coração e encéfalo desses animais. Foram utilizados 18 hamsters experimentalmente inoculados via intra-peritoneal, e seis animais sadios como controles negativos. Os animais foram mantidos em biotério de experimentação e observados, em busca de alterações clínicas. Com fragmentos de cada órgão, procedeu-se a confecção de lâminas por aposição para pesquisa de amastigotas. Nos órgãos com resultado negativo na pesquisa direta do parasito, e em todas as amostras de encéfalo, foi realizada a técnica de imunohistoquímica. A pesquisa direta de amastigotas foi positiva no baço e fígado de todos os animais infectados; 33,3% apresentaram o parasito em rim e pulmão, e 16,7% no coração. Quando realizada

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“…In humans there are reports of a few infected cells and amastigotes in interstitial tissue, alveolar septa and bronchoalveolar lavage (MELENEY, 1925;ANDRADE, 1959;JOKIPII et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Presence of amastigotes in the central nervous system of hamsters infected with Leishmania sp.

Oliveira

Oshiro

Pinto

et al. 2011

Rev. Bras. Parasitol. Vet.

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“…The risk of leishmaniasis among transplant recipients is associated with the geographic region in which donor and recipient live. However, few cases have been reported in transplant recipients from non-endemic areas that traveled to an endemic region of leishmaniasis 47,52 .…”

Section: Transmission In Transplant Patientsmentioning

confidence: 99%

Epidemiology of neglected tropical diseases in transplant recipients: review of the literature and experience of a Brazilian HSCT center

Machado

Martins

Colturato

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe rising success rate of solid organ (SOT) and haematopoietic stem cell transplantation (HSCT) and modern immunosuppression make transplants the first therapeutic option for many diseases affecting a considerable number of people worldwide. Consequently, developing countries have also grown their transplant programs and have started to face the impact of neglected tropical diseases (NTDs) in transplant recipients. We reviewed the literature data on the epidemiology of NTDs with greatest disease burden, which have affected transplant recipients in developing countries or may represent a threat to transplant recipients living in other regions. Tuberculosis, Leprosy, Chagas disease, Malaria, Leishmaniasis, Dengue, Yellow fever and Measles are the topics included in this review. In addition, we retrospectively revised the experience concerning the management of NTDs at the HSCT program of Amaral Carvalho Foundation, a public transplant program of the state of São Paulo, Brazil.

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“…Other forms in which the disease presents itself are granulomatous inflammation of the bronchial mucosa and mediastinal lymph nodes [86], and the development of pleural effusions with the presence of the intracellular protozoon in macrophages [87,88]. In other types of samples of the respiratory apparatus, such as transbronchial biopsy [89] and bronchoalveolar lavage [90,91], the presence of Leishmania amastigotes has also been detected.…”

Section: Genus Leishmaniamentioning

confidence: 99%

Protozoa in respiratory pathology: a review

Martínez‐Girón¹,

Esteban²,

Ribas³

et al. 2008

Eur Respir J

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Among the micro-organisms that may affect the respiratory apparatus are the protozoa. The diseases they may give rise to constitute a relatively uncommon group of respiratory ailments with, in the majority of cases, an underlying clinical situation corresponding to states of suppressed immunity (AIDS, transplants, malign haemopathies, corticotherapy, etc.). Other factors, such as visits to endemic areas and immigration, also have to be taken into account.In view of the probable increase in the number of cases and the appearance of new emerging diseases, it is the intention of the present work to review the publications available, in different fields of medicine, that refer to the principal kinds of protozoa (Entamoeba,

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Leishmaniasis Diagnosed from Bronchoalveolar Lavage

Cited by 33 publications

References 14 publications

Presence of amastigotes in the central nervous system of hamsters infected with Leishmania sp.

Presence of amastigotes in the central nervous system of hamsters infected with Leishmania sp.

Epidemiology of neglected tropical diseases in transplant recipients: review of the literature and experience of a Brazilian HSCT center

Protozoa in respiratory pathology: a review

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