Leishmaniose visceral canina em três bairros de Uruguaiana - RS

Massia, Laura Ilarraz; Lamadril, Rita Daniela Quevedo; Wellicks, José Ricardo; Bittencourt, Raqueli Altamiranda; Bittencourt, Danton Goulart; Marques, Gisele Dias; Celis, Eliana Leonor Hurtado; Pellegrini, Débora da Cruz Payão

doi:10.3395/2317-269x.00679

Cited by 3 publications

(4 citation statements)

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“…But there are probably many other positive animals that did not show clinical signs; therefore, the prevalence of dogs with present or past infection with L. infantum must be even higher. In a previous survey including 965 domestic dogs from this city, 4.5% of the animal samples presented antibodies against L. infantum between 2009 and 2010 [20]. In view of this concerning epidemiological situation with an increasing number of dogs with CanL, other studies have detected more animal species with L. infantum, including wild and domestic mammals [21,22].…”

Section: Discussionmentioning

confidence: 96%

Canine Leishmaniasis in Southern Brazil: Diagnosis and Clinical Features in Domestic Dogs

Fraga,

da Silveira,

Freitas Salla

et al. 2024

Zoonotic Diseases

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Leishmania infantum is a hemopathogen of importance for the health of domestic dogs (Canis lupus familiaris), causing canine leishmaniasis (CanL), and it is also the etiological agent of human visceral leishmaniasis (HVL). This parasite was not reported in southern Brazil until the early 2000s, but CanL and HVL were increasingly reported in the last 15 years, mainly in cities bordering Argentina. The present study aimed to detect L. infantum in domestic dogs and to determine the main clinical manifestations in infected animals from Uruguaiana, a city with a high incidence of CanL. Fifty-one dogs suspected of having CanL in the urban perimeter of the city were clinically examined by veterinarians and investigated for the occurrence of L. infantum with two immunoassays (rapid chromatography test and ELISA) and real-time PCR (polymerase chain reaction). Clinical signs were compared in positive and negative L. infantum animals. A total of 31 dogs (60.8%) were infected with L. infantum. The main clinical manifestations associated with CanL dogs were onychogryphosis and peeling (p < 0.05). L. infantum was frequently detected in urban dogs from Uruguaiana, highlighting the concerning situation regarding health in this city. The occurrence of some clinical signs (onychogryphosis/peeling) could help to detect CanL more frequently in the canine population.

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Section: Discussionmentioning

confidence: 96%

Canine Leishmaniasis in Southern Brazil: Diagnosis and Clinical Features in Domestic Dogs

Fraga,

da Silveira,

Freitas Salla

et al. 2024

Zoonotic Diseases

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“…Thirty-one (60.8%) of these 51 animals were confirmed to be positive for L. infantum. A previous survey had already reported a high frequency of CanL, since it was observed more than 10% of dog samples with antibodies against L. infantum between 2009 and 2010, with some neighborhoods in the city with higher frequencies than others [20]. In view of this concerning epidemiological situation, previous studies have already investigated other animal species with leishmaniosis, including wild and domestic mammals [21,22].…”

Section: Discussionmentioning

confidence: 99%

Canine Leishmaniasis in a Recent Endemic Region in Southern Brazil

Fraga,

Silveira,

Salla

et al. 2024

Preprint

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Leishmania infantum is an hemopathogen of importance for the health of domestic dogs (Canis lupus familiaris), causing canine leishmaniasis (CanL), as well as it is the etiological agent of human visceral leishmaniasis (HVL). This parasite was not reported in southern Brazil until the early 2000s, but CanL and HVL were increasingly reported in the last 15 years, mainly in cities bordering Argentina. The present study aimed to detect L. infantum in urban dogs and to determine the main clinical manifestations in the infected animals from Uruguaiana, a city with a high incidence of CanL. Fifty-one dogs suspected of having CanL in the urban perimeter of the city were clinically examined by veterinarians and investigated for the occurrence of L. infantum with two immunoassays (rapid chromatography test and ELISA) and real-time PCR. Clinical signs were compared in positive and negative L. infantum animals. A total of 31 dogs (60.8%) were infected with L. infantum. The main clinical manifestations associated to CanL dogs were onychogryphosis and peeling (p&lt;0.05). L. infantum was frequently detected in urban dogs from Uruguaiana, highlighting the health concerning situation in this city. The occurrence of some clinical signs (onychogryphosis/peeling) could help to detect more frequently CanL in the canine population.

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“…We found 12.9% of positive PCR among the dogs through using the primers RV1/RV2, and 37% through using the rapid test DPP. In an epidemiological survey carried out between years 2009 and 2010, by the health surveillance department of Uruguaiana, samples were collected from 965 dogs and 43 (4.4%) of these were seropositive for CVL (Massia et al, 2016). This study does not aim to elucidate the disease prevalence, but the characterization of circulating Leishmania spp., so, the technique and samples collection was different in the studies, precluding the percentage comparison.…”

Section: Discussionmentioning

confidence: 99%

“…At that time, when cases of both CVL and human VL (HVL) arose in this state, a serological survey was carried out among the dogs by the public healthcare service. From this, canine infection by Leishmania infantum was confirmed through the multilocus enzyme electrophoresis (MLEE), by the National Reference Laboratory for Leishmania Typing (LRNTL) (Massia et al, 2016) and this region was classified as a transmission area for CVL (SES, 2014;CEVS, 2021).…”

Section: Introductionmentioning

confidence: 99%

PCR-RLFP characterization of Leishmania spp. in domestic animals from the south-western border of Brazil

Pradella

Escobar

Santos

et al. 2022

Rev. Bras. Parasitol. Vet.

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The aim of this study was to characterize Leishmania spp. from canine and feline samples using Polymerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphism (RFLP). It was conducted in the southern region of Brazil, located at border crossings to Argentina and Uruguay. Samples were collected from 116 dogs (Canis lupus familiaris) and 89 cats (Felis catus). The PCR was performed to screen for an LT1 fragment from kinetoplast DNA (kDNA) target gene, and positive samples were subjected to a second PCR for an internal transcribed spacers (ITS1) region from ribosomal DNA (rDNA) target. RFLP was performed using the Haemophilus aegyptius (HAE III) restriction endonuclease (Fermentas ®). Positive samples by PCR ITS1 were sequenced and deposited in NCBI GenBank, and a phylogenetic analysis was developed. We found that 12.9% (15/116) of the samples from dogs were positive. All the 89 cat samples were negative. Positive samples were tested against Leishmania reference strains presenting different patterns in PCR-RFLP, and these samples showed bands denoting similarity to the standard species of Leishmania infantum, proven through sequencing and phylogenetic analysis. The RFLP technique, alone, was shown to be feasible for practical application and confirmation of the involved Leishmania spp.

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Leishmaniose visceral canina em três bairros de Uruguaiana - RS

Cited by 3 publications

References 0 publications

Canine Leishmaniasis in Southern Brazil: Diagnosis and Clinical Features in Domestic Dogs

Canine Leishmaniasis in Southern Brazil: Diagnosis and Clinical Features in Domestic Dogs

Canine Leishmaniasis in a Recent Endemic Region in Southern Brazil

PCR-RLFP characterization of Leishmania spp. in domestic animals from the south-western border of Brazil

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