Leishmanolysin gp63: Bioinformatics evidences of immunogenic epitopes in Leishmania major for enhanced vaccine design against zoonotic cutaneous leishmaniasis

Shams, Morteza; Nourmohammadi, Hassan; Basati, Gholam; Adhami, Ghazaaleh; Majidiani, Hamidreza; Azizi, Esfandiar

doi:10.1016/j.imu.2021.100626

Cited by 15 publications

(7 citation statements)

References 54 publications

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“…Based on such findings, it can be speculated that this protein could be a potential vaccine candidate. “From a biochemical standpoint, a protein is represented in four structural levels, comprising (i) amino acid sequences as primary structure, (ii) a native spatial form due to main chain atoms ( α -helix and β -fold) as secondary structure, (iii) potential spatial model as a 3D model or tertiary structure, and (iv) number and position of multifold subunits in a multisubunit collection of a protein as quaternary structure” [ 34 – 36 ]. In the first step of this study, we characterized general biochemical features of the protein.…”

Section: Discussionmentioning

confidence: 99%

Determination of B and T Cell Epitopes in Neospora caninum Immune Mapped Protein-1 (IMP-1): Implications in Vaccine Design against Neosporosis

Nazari

Kordi

Maleki

et al. 2022

BioMed Research International

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Prevention of neosporosis is advantageous for cattle health and productivity. Previously, several vaccine candidates were nominated for vaccination against Neospora caninum. This study was premised on in silico evaluation of N. caninum IMP-1 in order to determine its physicochemical features and immunogenic epitopes. We employed a wide array of network-based tools for the prediction of antigenicity, allergenicity, solubility, posttranslational modification (PTM) sites, physicochemical properties, transmembrane domains and signal peptide, secondary and tertiary structures, and intrinsically disordered regions. Also, prediction and screening of potential continuous B cell peptides and those epitopes having stringent affinity to couple with mouse major histocompatibility complex (MHC) and cytotoxic T lymphocyte (CTL) receptors were accomplished. The protein had 393 residues with a molecular weight of 42.71 kDa, representing aliphatic index of 85.83 (thermotolerant) and GRAVY score of -0.447 (hydrophilic). There were 47 PTM sites without a signal peptide in the sequence. Secondary structure comprised mostly of extended strand and helices, followed by coils. The Ramachandran plot of the refined model showed 90.1%, 9.9%, 0.0%, and 0.0% residues in the favored, additional allowed, generously allowed, and disallowed regions, correspondingly. Additionally, various potential B cell (linear and conformational), CTL, and MHC binding epitopes were predicted for N. caninum IMP-1. The findings of the present study could be further directed for next-generation vaccine design against neosporosis.

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Section: Discussionmentioning

confidence: 99%

Determination of B and T Cell Epitopes in Neospora caninum Immune Mapped Protein-1 (IMP-1): Implications in Vaccine Design against Neosporosis

Nazari

Kordi

Maleki

et al. 2022

BioMed Research International

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“…Additionally, we also identified Biopterin Transporter (BT1) overexpression in this stage, which product plays a key role in growth, infectivity and survival in the macrophages (94). In two computational studies, the potential of the GP63 protein from L. major and L. donovani as a vaccine target was demonstrated (95,96). Another study performed by Chowdhury and cols.…”

Section: Discussionmentioning

confidence: 99%

Comparative and systems analyses of Leishmania spp. non-coding RNAs through developmental stages

Martínez-Hernández

Aliaga-Tobar

González

et al. 2021

Preprint

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Leishmania spp. is the causal agent of several diseases called leishmaniases, neglected diseases that seek to be eradicated in the coming years. We aimed to study the genomic structure and function of non-coding RNAs (ncRNAs) from Leishmania spp. and to get insights into its RNAome. We studied 26 strains corresponding to 16 different species of Leishmania genus. RNAome analysis revealed the presence of several ncRNAs that are shared through different species, allowing us to differentiate between subgenus and as well as species that are canonically related to visceral leishmaniasis. We found coexpression relationships within coding genes and ncRNAs, thus suggesting possible functional relationships between different coding genes-ncRNAs. Expression analysis in the metacyclic developmental stage comparison for Leishmania braziliensis and Leishmania major reveals the presence of shared coexpressed ncRNAs to several other coding genes in both species involved in chromatin structure and host interaction. This work constitutes the first effort to characterize the Leishmania RNAome, supporting further approaches to better understand the role of ncRNAs in the gene regulation, infective process and host-parasite interaction.

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“…A multistep approach was exploited for linear B-cell epitope prediction in NcSRS2. For this aim, a fixed-length prediction (14-mer) with 75% specificity was applied in BCPREDS server ( http://ailab.ist.psu.edu/bcpred/predict.html ), which uses subsequent kernel (SSK) and support vector machine (SVM) techniques [ 44 – 46 ]. In the next step, cross-validation of the predicted epitopes was accomplished with the outputs of two other web servers, including ABCpred ( http://crdd.osdd.net/raghava/abcpred/ABC_submission ) [ 47 ] and SVMTriP ( http://sysbio.unl.edu/SVMTriP/prediction.php ) [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Neospora caninum SRS2 Protein: Essential Vaccination Targets and Biochemical Features for Next-Generation Vaccine Design

Asghari

Kordi

Maleki

et al. 2022

BioMed Research International

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Vaccination is a standout preventive measure to combat neosporosis among cattle herds. The present in silico study was done to evaluate the physicochemical properties and potent immunogenic epitopes of N. caninum SRS2 protein as a possible vaccine candidate. Web-based tools were used to predict physicochemical properties, antigenicity, allergenicity, solubility, posttranslational modification (PTM) sites, transmembrane domains and signal peptide, and secondary and tertiary structures as well as intrinsically disordered regions, followed by identification and screening of potential linear and conformational B-cell epitopes and those peptides having affinity to bind mouse major histocompatibility complex (MHC) and cytotoxic T lymphocyte (CTL). The protein had 401 residues with a molecular weight of 42 kDa, representing aliphatic index of 69.35 (thermotolerant) and GRAVY score of -0.294 (hydrophilic). There were 53 PTM sites without a signal peptide in the sequence. Secondary structure comprised mostly by extended strand, followed by helices and coils. The Ramachandran plot of the refined model showed 90.2%, 8.8%, 0.5%, and 0.5% residues in the favored, additional allowed, generously allowed, and disallowed regions, correspondingly. Additionally, various potential B-cell (linear and conformational), CTL, and MHC-binding epitopes were predicted for N. caninum SRS2. These epitopes could be further utilized in the multiepitope vaccine constructs directed against neosporosis.

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Leishmanolysin gp63: Bioinformatics evidences of immunogenic epitopes in Leishmania major for enhanced vaccine design against zoonotic cutaneous leishmaniasis

Cited by 15 publications

References 54 publications

Determination of B and T Cell Epitopes in Neospora caninum Immune Mapped Protein-1 (IMP-1): Implications in Vaccine Design against Neosporosis

Determination of B and T Cell Epitopes in Neospora caninum Immune Mapped Protein-1 (IMP-1): Implications in Vaccine Design against Neosporosis

Comparative and systems analyses of Leishmania spp. non-coding RNAs through developmental stages

Neospora caninum SRS2 Protein: Essential Vaccination Targets and Biochemical Features for Next-Generation Vaccine Design

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