Lelliottia jeotgali sp. nov., isolated from a traditional Korean fermented clam

Abstract

Strain PFL01 was isolated from traditional Korean fermented clam, jogae-jeotgal, and characterized. The strain was a facultative anaerobic, Gram-stain-negative bacterium that was rod-shaped, motile and beige-pigmented. The phylogenetic sequence analysis based on the 16S rRNA gene from PFL01 revealed that it was closely related to Lelliottia nimipressuralis LMG 10245 and Lelliottia amnigena LMG 2784 with 99.3 and 99.3 % sequence identities, respectively. Multilocus sequence type analysis of concatenated partial aptD, gyrB, infB and rpoB gene sequences showed a clear distinction of strain PFL01 from its closest related type strains. The discrimination was also supported by unique repetitive extragenic palindromic PCR (Rep-PCR, ERIC-PCR) fingerprint patterns. In addition, results from average nucleotide identity analyses with other species were less than 85 %. vitek and API analyses revealed distinct characteristics from other species of Lelliottia. The cellular fatty acid profile of the strain consisted of C16 : 0, cyclo-C17 : 0, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c as major components. The whole genome of PFL01 was 4.6 Mb with a G+C content of 55.3 mol%. Based on these results, strain PFL01 was classified as a novel species of the genus Lelliottia, for which the name Lelliottia jeotgali sp. nov. is proposed. The type strain in PFL01 (=KCCM 43247=JCM 31901).

iac Gene Expression in the Indole-3-Acetic Acid-Degrading Soil Bacterium Enterobacter soli LF7

“…Interestingly, the exact same gene arrangement can be found on the genomes of other Enterobacteriaceae, including Lelliottia sp. PFL01 (27), Leclercia sp. LSNIH1 and LSNIH3 (28), and Leclercia adecarboxylata strain USDA-ARS-USMARC-60222 (29).…”

“…LSNIH1 and LSNIH3 (28), and Leclercia adecarboxylata strain USDA-ARS-USMARC-60222 (29). However, the environments from which these particular strains were isolated are very different, i.e., fermented seafood (27), hospital plumbing (28), and livestock (29), respectively. This confirms that while the possession of iac genes is a good predictor of its host being able to grow at the expense of the plant hormone IAA (as shown for E. soli LF7 here), it is not prognostic of a plant-environmental origin of the iac-carrying bacterial host.…”

Genome analysis-based reclassification of Lelliottia aquatilis as a later heterotypic synonym of Lelliottia jeotgali

“…Growth at NaCl (w/v) 0-9 % 0-9 % 0-9 % 0-9 % 0-9 % 0-8 % †The G+C contents and N50 were calculated from the whole genome sequences of these strains using Quast (http://quast.bioinf.spbau.ru). ‡The complete sequence of the whole genome of L. jeotgali PFL01 T , which was obtained using PacBio RS II (Pacific Biosciences), contains a single chromosome and no plasmids [2]. The five L. aquatilis strains have only draft genome sequences available, which was obtained using MiSeq (Illumina) [3].…”

“…The morphological characteristics of the two type strains, L. aquatilis 6331-17 T and L. jeotgali PFL01 T are similar, such as Gram-stain-negative, motile, rod-shaped, facultative anaerobic and beige-pigmented colonies formed on nutrient agar [2,3]. The physiological and chemotaxonomic features of the two type strains and other strains of L. aquatilis were compared based on the previous data [2,3] with detailed information in Table 1. The six strains (including two type strains) have overall similar phenotypic characteristics.…”

“…This suggests that the catalase reaction of strains of this species could vary but the variation may also be due to differences in conditions to test the catalase reaction. Unfortunately, the methodology for testing catalase reaction was not described in detail [2,3] and we recommend that the authors of the two studies repeat the tests. In addition, all L. aquatilis strains had acid production from D-sorbitol and four of the five L. aquatilis strains produced acid from lactose and salicin, while the L. jeotgali strain did not utilize the three compounds.…”