2006
DOI: 10.1007/s00425-006-0286-0
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LeMAN4 endo-β-mannanase from ripe tomato fruit can act as a mannan transglycosylase or hydrolase

Abstract: Mannan transglycosylases are cell wall enzymes able to transfer part of the mannan polysaccharide backbone to mannan-derived oligosaccharides (Schröder et al. in Planta 219:590-600, 2004). Mannan transglycosylase activity was purified to near homogeneity from ripe tomato fruit. N-terminal sequencing showed that the dominant band seen on SDS-PAGE was identical to LeMAN4a, a hydrolytic endo-beta-mannanase found in ripe tomato fruit (Bewley et al. in J Exp Bot 51:529-538, 2000). Recombinant LeMAN4a protein expres… Show more

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Cited by 62 publications
(59 citation statements)
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“…However, besides xyloglucans, only one other class of cell-wall polysaccharide -(1 fi 4)-b-D-mannans -is a known target of endotransglycosylases (Schrö der et al, 2004(Schrö der et al, , 2006. No known endotransglycosylases target pectins (García-Romera and Fry, 1994) or xylans.…”
Section: Introductionmentioning
confidence: 99%
“…However, besides xyloglucans, only one other class of cell-wall polysaccharide -(1 fi 4)-b-D-mannans -is a known target of endotransglycosylases (Schrö der et al, 2004(Schrö der et al, , 2006. No known endotransglycosylases target pectins (García-Romera and Fry, 1994) or xylans.…”
Section: Introductionmentioning
confidence: 99%
“…Fruit LeMAN4a was the first (and currently the only) enzyme identified that exhibits both MAN and mannan transglycosylase activity. However, its physiological role has not been yet clarified (Schroder et al 2006). The dual characteristic of AtMAN is currently under study.…”
Section: Discussionmentioning
confidence: 99%
“…Existing assay methods can be subdivided into viscometric [21], radiometric [4,12,35], fluorimetric [11] and colorimetric [37]. Separation of reaction products can be achieved by size-exclusion HPLC [24,25], paper chromatography [30] or high-performance capillary electrophoresis [29]. Unfortunately, none of these approaches is conducive to simultaneous, monitoring of multiple transglycosylase reactions.…”
Section: Introductionmentioning
confidence: 99%