Lemon grass (Cymbopogon citratus (D.C) Stapf) polyphenols protect human umbilical vein endothelial cell (HUVECs) from oxidative damage induced by high glucose, hydrogen peroxide and oxidised low-density lipoprotein

Campos, J.; Schmeda-Hirschmann, Guillermo; Leiva, Elba; Guzmán, Luís; Orrego, Roxana; Fernández, P; González, Marcelo; Radojkovic, Claudia; Zúñiga, Felipe; Lamperti, Liliana; Pastene, Edgar; Aguayo, Claudio

doi:10.1016/j.foodchem.2013.11.018

Cited by 69 publications

(52 citation statements)

References 46 publications

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“…In addition, H 2 O 2 is a major component of ROS produced intracellularly during a number of physiological and pathological processes, and causes oxidative damage (25,26). For this reason, H 2 O 2 has often been used as an experimental model to investigate the mechanisms of cell injury induced by oxidative stress (27)(28)(29). In the present study, when HepG2 cells pre-treated with FBG were challenged with H 2 O 2 , ROS formation decreased.…”

Section: Discussionmentioning

confidence: 65%

Detoxifying effect of fermented black ginseng on H2O2-induced oxidative stress in HepG2 cells

Bak

Jeong

Kim

2014

International Journal of Molecular Medicine

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Abstract. Fermented black ginseng (FBG) is prepared by repeated steaming and drying processes with fresh ginseng followed by fermentation with Saccharomyces cerevisiae. It has recently been shown to have several bioactivities. FBG contains crude saponin (1,440 µg/ml), ginsenoside Rg2 (2.86 µg/ml), ginsenoside Rg3 (24.52 µg/ml), ginsenoside Rh1 (12.64 µg/ml), ginsenoside Rh2 (0.63 µg/ml) and ginsenoside Rf (1.32 µg/ml). The present study investigated the antioxidant defense properties of FBG against hydrogen peroxide (H 2 O 2 )-mediated oxidative stress in HepG2 human hepatocellular carcinoma cells. The increased production of reactive oxygen species (ROS) induced by H 2 O 2 was attenuated in a dose-dependent manner when the cells were pre-treated with FBG (10-50 µg/ml). FBG induced both the expression and activity of antioxidant enzymes, such as superoxide dismutase, catalase and glutathione peroxidase in the H 2 O 2 -treated HepG2 cells. The inhibitory effects of FBG on the phosphorylation of upstream mitogen-activated protein kinases (MAPKs), such as c-Jun N-terminal kinase, extracellular signal-regulated kinase and p38 were also observed. Overall, our results demonstrate that FBG protects HepG2 cells from oxidative stress through the induction of antioxidant enzyme activity and the inhibition of MAPK pathways.

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Section: Discussionmentioning

confidence: 65%

Detoxifying effect of fermented black ginseng on H2O2-induced oxidative stress in HepG2 cells

Bak

Jeong

Kim

2014

International Journal of Molecular Medicine

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“…51,52 For example, expression of tissue factor is induced by mildly oxidized LDL, but not by highly oxidized LDL in endothelial cells. 53 In this scenario, we acknowledge that one of the limitations in our experiments is lack of characterization of the degree of oxidation in the ox-LDL molecule; however, we have used a well-characterized methodology, cupper-induced LDL oxidation, 54 so we can make the assumption that we used medially-oxidized LDL at concentrations similar to those used by previous studies. 26,54 …”

Section: Lox-1 and Hepcmentioning

confidence: 99%

Overexpression of LOXIN Protects Endothelial Progenitor Cells From Apoptosis Induced by Oxidized Low Density Lipoprotein

Veas

Jara

Willis

et al. 2016

Journal of Cardiovascular Pharmacology

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Human endothelial progenitor cells (hEPC) are adult stem cells located in the bone marrow and peripheral blood. Studies have indicated that hEPC play an important role in the recovery and repair of injured endothelium, however, their quantity and functional capacity is reduced in several diseases including hypercholesterolemia. Recently, it has been demonstrated that hEPC express lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and its activation by oxidized low-density lipoprotein (ox-LDL) induces cellular dysfunction and apoptosis. This study aimed to investigate whether overexpression of LOXIN, a truncated isoform of LOX-1 that acts as a dominant negative, plays a protective role against ox-LDL-induced apoptosis in hEPC. Human endothelial progenitor cells exposed to ox-LDL showed a significant increase in LOX-1 expression, and apoptosis began at ox-LDL concentrations above 50 μg/mL. All hEPC apoptosed at 200 μg/mL ox-LDL. High LOXIN expression was generated using adenoviral systems in hEPC and SiHa cells transduced with 100 colony-forming units per cell. Transduced LOXIN localized to the plasma membrane and blocked ox-LDL uptake mediated by LOX-1. Overexpression of LOXIN protected hEPC from ox-LDL-induced apoptosis, and therefore maybe a novel way of improving hEPC function and quantity. These results suggest that adenoviral vectors of LOXIN may provide a possible treatment for diseases related to ox-LDL and vascular endothelium dysfunction, including atherosclerosis.

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“…1,2 The volatile oil obtained from the fresh leaves of the plant is one of the most important essential oils in the food, cosmetics, and pharmaceutical industries. The importance of lemongrass oil (LGO) in the pharmaceutical industry is a result of its various pharmacologic and clinical effects.…”

Section: Introductionmentioning

confidence: 99%

Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation

Aldawsari¹,

Badr-Eldin²,

Labib³

et al. 2015

IJN

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Lemongrass oil (LGO) is a volatile oil extracted from the leaves of Cymbopogon citratus that has become one of the most important natural oils in the pharmaceutical industry because of its diverse pharmacologic and clinical effects. However, LGO suffers from low aqueous solubility, which could lead to a reduced effect. Moreover, the instability of its major active constituent, citral, could lead to volatilization, reaction with other formulation ingredients, and consequently, skin irritation. To surmount these problems, this research aims to formulate lemongrass-loaded ethyl cellulose nanosponges with a topical hydrogel with an enhanced antifungal effect and decreased irritation. The minimal inhibitory concentration and minimal fungicidal concentration of LGO against Candida albicans strain ATC 100231, determined using the broth macrodilution method, were found to be 2 and 8 μL/mL, respectively. The emulsion solvent evaporation technique was used for the preparation of the nanosponges. The nanosponge dispersions were then integrated into carbopol hydrogels (0.4%). Nine formulations were prepared based on a 3 2 full factorial design employing the ethyl cellulose:polyvinyl alcohol ratio and stirring rate as independent variables. The prepared formulations were evaluated for particle size, citral content, and in vitro release. Results revealed that all the nanosponge dispersions were nanosized, with satisfactory citral content and sustained release profiles. Statistical analysis revealed that both ethyl cellulose:polyvinyl alcohol ratio and stirring rate have significant effects on particle size and percentage released after 6 hours; however, the effect of the stirring rate was more prominent on both responses. The selected hydrogel formulation, F9, was subjected to surface morphological investigations, using scanning and transmission electron microscopy, where results showed that the nanosponges possess a spherical uniform shape with a spongy structure, the integrity of which was not affected by integration into the hydrogel. Furthermore, the selected formulation, F9, was tested for skin irritation and antifungal activity against C. albicans , where results confirmed the nonirritancy and the effective antifungal activity of the prepared hydrogel.

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Lemon grass (Cymbopogon citratus (D.C) Stapf) polyphenols protect human umbilical vein endothelial cell (HUVECs) from oxidative damage induced by high glucose, hydrogen peroxide and oxidised low-density lipoprotein

Cited by 69 publications

References 46 publications

Detoxifying effect of fermented black ginseng on H2O2-induced oxidative stress in HepG2 cells

Detoxifying effect of fermented black ginseng on H2O2-induced oxidative stress in HepG2 cells

Overexpression of LOXIN Protects Endothelial Progenitor Cells From Apoptosis Induced by Oxidized Low Density Lipoprotein

Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation

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Lemon grass (Cymbopogon citratus (D.C) Stapf) polyphenols protect human umbilical vein endothelial cell (HUVECs) from oxidative damage induced by high glucose, hydrogen peroxide and oxidised low-density lipoprotein

Cited by 69 publications

References 46 publications

Detoxifying effect of fermented black ginseng on H2O2-induced oxidative stress in HepG2 cells

Detoxifying effect of fermented black ginseng on H2O2-induced oxidative stress in HepG2 cells

Overexpression of LOXIN Protects Endothelial Progenitor Cells From Apoptosis Induced by Oxidized Low Density Lipoprotein

Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in&nbsp;vivo&nbsp;evaluation

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Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation