Length-weight relationship (LWR) can be used to estimate the weight or length of fish species when only one of these values is known. The measurement of these biometric data (weight and length) is routine in studies that monitor fauna and, according to Zuchi et al. (2020), these data are easily obtained.The Negro River is the second largest sub-basin of the Amazon basin (Goulding et al., 2003). In this sub-basin, currently 1,233 species are known (Jézéquel et al., 2020) to inhabit different environments such as rivers, lakes, flooded forests (igapós), beaches, intersecting river channels (paranás) and streams (igarapés) (Beltrão et al., 2019). Among these large number of rivers and other important environments, we highlight the Cuiuni River and the Anavilhanas National Park. The Cuiuni River is located the middle stretch of the Negro River basin, and is one of the largest southern tributaries of the Negro River (Montero & Latrubesse, 2013). According to Junk et al. (2015), this river drains the floodplains (várzea) and paleofloodplain (paleo-várzea) from the Japurá River, which is a tributary of the left bank of the Solimões/Amazonas River. While, the Anavilhanas National Park is a conservation unit, situated in the lower stretch of the Negro River basin (Amazonas state, Brazil). It is the second largest fluvial archipelago in the world and is constituted by approximately 400 islands (Almeida-Filho & Miranda, 2007). The lakes in the Anavilhanas National Park are separated by fluvial islands and connected by channels, and the lakes are typical of black water rivers and show the same general hydrological and limnological characteristics (Goulding et al., 1988).Therefore, because they have different characteristics, these environments allow diversification of fish species. Despite the increase in descriptions of new species, there are few studies published that provide information on the parameters of the length-weight relationship