Lengths and nucleotide sequences of the internal spacers of nuclear ribosomal DNA in gymnosperms and pteridophytes

Maggini, F.; Marrocco, R.; Gelati, M. T.; Dominicis, Raffaele Ilio De

doi:10.1007/bf00985200

Cited by 25 publications

(20 citation statements)

References 15 publications

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“…We explored the DNA sequences for the presence of several structural motifs. Thus, at the ITS1 region we searched for the presence and length of the conserved angiosperm motif (Liu and Schardl, 1994) GGCRY-(4 to 7n)-GYGYCAAGGAA (recently also found in several gymnosperms; Maggini et al, 1998). This core sequence is most often associated with part of a hairpin structure, whereas the AAGGAA motif is predicted to stand as a non-base-paired sequence in an internal loop structure (Fig.…”

Section: Methodsmentioning

confidence: 99%

Why Nuclear Ribosomal DNA Spacers (ITS) Tell Different Stories in Quercus

Mayol

Rosselló

2001

Molecular Phylogenetics and Evolution

151

108

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Section: Methodsmentioning

confidence: 99%

Why Nuclear Ribosomal DNA Spacers (ITS) Tell Different Stories in Quercus

Mayol

Rosselló

2001

Molecular Phylogenetics and Evolution

151

108

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“…In gymnosperms, the ITSl sequences are generally much longer than in angiosperms, whereas the length of ITS2 shows a similar range of variation as in angiosperms (Liston et al, 1996, Maggini et al, 1998. The first, named ITS1, is localised between the 185 and 5.8s regions and the second, named ITS2, is localised between 5.8s and 25s regions.…”

mentioning

confidence: 97%

“…The shorter ITSl is characterized by three shorter subrepeats: ssrl, ssr2 and ssr3, 221 bp, 227 bp and 226 bp long respectively. Sequence analysis of the ITSl from Pinus pinea, Epkedra fragilis, Larix and Pseudotsuga showed the presence of several tandem or dispersal sub-repeats of different size (Marrocco et aZ., 1996;Maggini et al, 1998; Gernardt and Liston, 1999). The similarity between the three ssr range from 66% to 79% and between the three LSR range from 65% to 96%.…”

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confidence: 99%

Nucleotide Sequence of the Internal Transcribed Spacers of Ribosomal DNA inPicea AbiesKarst

Maggini¹,

Frediani²,

Gelati³

2000

DNA Sequence

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The nucleotide sequences of the internal transcribed spacers (ITS1 and ITS2) of ribosomal DNA from Picea abies are reported. Two types of ITS1 of 2784 bp and 3271 bp long exist, whereas only one ITS2 type 238 bp long is present in this species. The shorter ITS1 is characterized by three shorter subrepeats: ssr1, ssr2 and ssr3, 221 bp, 227 bp and 226 bp long respectively. Between the ssr1 and ssr2 sub-repeats are inserted three longer sub-repeats: LSR1, LSR2 and LSR3, 480 bp, 480 bp and 581 bp long respectively. The similarity between the three ssr range from 66% to 79% and between the three LSR range from 65% to 96%. At the end of the LSR3 a microsatellite of 14 CT elements is present. The longer ITS1 type is due to a duplication of the LSR1, most probably obtained by unequal crossing-over and it has an identity of 97.3% with the shorter ITS1 type.

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“…Gernandt and Liston 1999;Liston et al 1999;Gernandt et al 2001;Wei and Wang 2004). In the Pinaceae, the ITS region is longer than in all other vascular plants, and the ITS1 region has distinctive subrepeats (Liston et al 1996;Marrocco et al 1996;Maggini et al 1998;Gernandt and Liston 1999;Vining 1999;Gernandt et al 2001;Wei et al 2003;Campbell et al 2005, Kan et al 2007). In addition, ITS copies within a species do not always coalesce (Gernandt and Liston 1999;Wei and Wang 2004;Campbell et al 2005).…”

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confidence: 98%

Phylogeny and Biogeography of Tsuga (Pinaceae) Inferred from Nuclear Ribosomal ITS and Chloroplast DNA Sequence Data

et al. 2008

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Hemlock, Tsuga (Pinaceae), has a disjunct distribution in North America and Asia. To examine the biogeographic history of Tsuga, phylogenetic relationships among multiple accessions of all nine species were inferred using chloroplast DNA sequences and multiple cloned sequences of the nuclear ribosomal ITS region. Analysis of chloroplast and ITS sequences resolve a clade that includes the two western North American species, T. heterophylla and T. mertensiana, and a clade of Asian species within which one of the eastern North American species, T. caroliniana, is nested. The other eastern North American species, T. canadensis, is sister to the Asian clade. Tsuga chinensis from Taiwan did not group with T. chinensis from mainland China, and T. sieboldii from Ullung Island did not group with T. sieboldii from Japan suggesting that the taxonomic status of these distinct populations should be reevaluated. The Himalayan species, T. dumosa, was in conflicting positions in the chloroplast and ITS trees, suggesting that it may be of hybrid origin. Likelihood-based biogeographic inference with divergence time estimates infers an Eocene basal crown group diversification and an initial widespread circumpolar distribution with subsequent vicariance and extinction events leading to the current disjunct distribution.

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Lengths and nucleotide sequences of the internal spacers of nuclear ribosomal DNA in gymnosperms and pteridophytes

Cited by 25 publications

References 15 publications

Why Nuclear Ribosomal DNA Spacers (ITS) Tell Different Stories in Quercus

Why Nuclear Ribosomal DNA Spacers (ITS) Tell Different Stories in Quercus

Nucleotide Sequence of the Internal Transcribed Spacers of Ribosomal DNA inPicea AbiesKarst

Phylogeny and Biogeography of Tsuga (Pinaceae) Inferred from Nuclear Ribosomal ITS and Chloroplast DNA Sequence Data

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