This study proposes a parabolic off-axis reflective optical system design method to reduce the wave aberration of the optical system of a large aperture single star simulator and improve the optical system’s imaging quality. Firstly, we determined the design indexes of the optical system of the large aperture single star simulator by analyzing the technical indexes of the star sensitizer and the development status of the single star simulator; secondly, the initial structural parameters of the optical system were calculated based on the theory of primary aberration; then, we carried out the design optimization of the optical system, the image quality evaluation, and the tolerance analysis using Zemax software; finally, the study tested the wave aberration of the optical system by using the four-dimensional interferometer and the standard mirror together. The simulation results of the optical system are as follows: in the entire field of view, the aberration of the optical system is far less than 0.002%, the modulation transfer function (MTF) reaches the diffraction limit, and the maximum wave aberration is 0.0324 λ. The experimental results are as follows: the maximum wave aberration of the optical system is 0.0337 λ, which is less than 1/25 λ, and it meets the requirements of the index. The simulation and experimental results show that the optical system of the large aperture single star simulator designed by this method has good imaging quality and a simple system structure.