Lens-Free Imaging for Biological Applications

Kim, Sang Bok; Bae, Hojae; Koo, Kyo‐in; Dokmeci, Mehmet R.; Özcan, Aydogan; Khademhosseini, Ali

doi:10.1177/2211068211426695

Cited by 64 publications

(39 citation statements)

References 48 publications

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“…These major limitations in resolution, FOV and DOF of traditional lens-based direct imaging sensors pave the way for applying lens-less microscopy techniques to wear debris monitoring. Lens-less microscopy is an emerging cost-effective technique that enables capturing high resolution images of 1 μm or below, over large FOV and long DOF with simple, compact and lightweight settings [24,25]. …”

Section: Introductionmentioning

confidence: 99%

Photonic Low Cost Micro-Sensor for in-Line Wear Particle Detection in Flowing Lube Oils

Mabe

Zubía

Gorritxategi³

2017

Sensors

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The presence of microscopic particles in suspension in industrial fluids is often an early warning of latent or imminent failures in the equipment or processes where they are being used. This manuscript describes work undertaken to integrate different photonic principles with a micro- mechanical fluidic structure and an embedded processor to develop a fully autonomous wear debris sensor for in-line monitoring of industrial fluids. Lens-less microscopy, stroboscopic illumination, a CMOS imager and embedded machine vision technologies have been merged to develop a sensor solution that is able to detect and quantify the number and size of micrometric particles suspended in a continuous flow of a fluid. A laboratory test-bench has been arranged for setting up the configuration of the optical components targeting a static oil sample and then a sensor prototype has been developed for migrating the measurement principles to real conditions in terms of operating pressure and flow rate of the oil. Imaging performance is quantified using micro calibrated samples, as well as by measuring real used lubricated oils. Sampling a large fluid volume with a decent 2D spatial resolution, this photonic micro sensor offers a powerful tool at very low cost and compacted size for in-line wear debris monitoring.

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Section: Introductionmentioning

confidence: 99%

Photonic Low Cost Micro-Sensor for in-Line Wear Particle Detection in Flowing Lube Oils

Mabe

Zubía

Gorritxategi³

2017

Sensors

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“…For example, compact image sensors are widely applicable to POC analysis at mammalian cell level [4–9]. The mini-microscopy has several key advantages over conventional microscopy in undeveloped regions [10], including: i ) simplicity, light weight, and compactness: a mini-microscope can be easily constructed with simple design, making the system compact and portable, well suited for POC diagnostics; ii ) cost-effectiveness: the main components of a mini-microscope include off-the-shelf items such as a light-emitting diode (LED) and an image sensor, making it affordable in POC diagnostics. Nevertheless, while the mini-microscopes are widely applicable in portable POC diagnosis at the scale of mammalian cells, it remains challenging for their utility in detecting disease-causing pathogens ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Expansion mini-microscopy: An enabling alternative in point-of-care diagnostics

Zhang

Santiago

Álvarez

et al. 2017

Current Opinion in Biomedical Engineering

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Diagnostics play a significant role in health care. In the developing world and low-resource regions the utility for point-of-care (POC) diagnostics becomes even greater. This need has long been recognized, and diagnostic technology has seen tremendous progress with the development of portable instrumentation such as miniature imagers featuring low complexity and cost. However, such inexpensive devices have not been able to achieve a resolution sufficient for POC detection of pathogens at very small scales, such as single-cell parasites, bacteria, fungi, and viruses. To this end, expansion microscopy (ExM) is a recently developed technique that, by physically expanding preserved biological specimens through a chemical process, enables super-resolution imaging on conventional microscopes and improves imaging resolution of a given microscope without the need to modify the existing microscope hardware. Here we review recent advances in ExM and portable imagers, respectively, and discuss the rational combination of the two technologies, that we term expansion mini-microscopy (ExMM). In ExMM, the physical expansion of a biological sample followed by imaging on a mini-microscope achieves a resolution as high as that attainable by conventional high-end microscopes imaging non-expanded samples, at significant reduction in cost. We believe that this newly developed ExMM technique is likely to find widespread applications in POC diagnostics in resource-limited and remote regions by expanded-scale imaging of biological specimens that are otherwise not resolvable using low-cost imagers.

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“…One approach is to apply traditional imaging tools where light propagates in free space and interacts with cells positioned on its path. For instance, imaging using traditional cell phone cameras has been demonstrated as a cost-effective solution that could be integrated into cell culture incubators to monitor cellular responses induced by drugs and other stimuli (Kim et al 2011; Kim et al 2012; Zheng et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior

Shevchenko

Camci‐Unal

Cuttica

et al. 2014

Biosensors and Bioelectronics

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104

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This paper reports on the application of an optical fiber biosensor for real-time analysis of cellular behavior. Our findings illustrate that a fiber sensor manufactured from a traditional telecommunication fiber can be integrated into conventional cell culture equipment and used for real-time and label-free monitoring of cellular responses to chemical stimuli. The sensing mechanism used for the measurement of cellular responses is based on the excitation of Surface Plasmon Resonance (SPR) on the surface of the optical fiber. In this proof of concept study, the sensor was utilized to investigate the influence of a number of different stimuli on cells - we tested the effects of trypsin, serum and sodium azide. These stimuli induced detachment of cells from the sensor surface, uptake of serum and inhibition of cellular metabolism, accordingly. The effects of different stimuli were confirmed with alamar blue assay, phase contrast and fluorescence microscopy. The results indicated that the fiber biosensor can be successfully utilized for real-time and label-free monitoring of cellular response in the first 30 minutes following the introduction of a stimulus. Furthermore, we demonstrated that the optical fiber biosensors can be easily regenerated for repeated use, proving this platform as a versatile and cost-effective sensing tool.

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Lens-Free Imaging for Biological Applications

Cited by 64 publications

References 48 publications

Photonic Low Cost Micro-Sensor for in-Line Wear Particle Detection in Flowing Lube Oils

Photonic Low Cost Micro-Sensor for in-Line Wear Particle Detection in Flowing Lube Oils

Expansion mini-microscopy: An enabling alternative in point-of-care diagnostics

Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior

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