Lensless coherent imaging of proteins and supramolecular assemblies: Efficient phase retrieval by the charge flipping algorithm

Dumas, Christian; Lee, Arie van der; Palatinus, Lukáš

doi:10.1016/j.jsb.2013.01.008

Cited by 3 publications

(3 citation statements)

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“…Short wavelength extreme ultraviolet (EUV) and soft X-ray beams have unique potential for imaging reactions at buried interfaces because they can penetrate visibly opaque materials, provide chemically specific contrast, and also image nanoscale features. In particular, by combining coherent beams from either high harmonic generation (HHG) or X-ray free electron lasers (XFELs) with coherent diffractive imaging (CDI), − it is now possible to achieve spatial resolutions that are comparable to the illuminating wavelength in the X-ray region for the first time. , Accordingly, CDI has found a range of applications in transmission and reflection geometries − to investigate nanoscale strain, , semiconductor structures, and for biological imaging. − Because no optical elements are needed between the sample and detector, coherent diffractive imaging is the most photon efficient form of imaging and can be nondestructive with no charging effects or resolution loss with depth . Moreover, the contrast mechanisms in EUV CDI are relatively straightforward and intrinsically high: amplitude images exhibit high sensitivity to material composition, while phase images are exquisitely sensitive to both material composition and topography.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, by combining coherent, short-wavelength beams from either high harmonic generation (HHG) [10] or X-ray free electron lasers (XFELs) [11] with coherent diffractive imaging (CDI) [12][13][14][15], it is now possible to reach near-wavelength resolution imaging in the EUV and X-ray regions for the first time [16,17]. Accordingly, CDI has found a range of applications in transmission and reflection geometries [18][19][20] to investigate nanoscale strain [21,22], semiconductor structures [18], and for biological imaging [23][24][25]. EUV/X-ray CDI can be non-destructive and suffers no charging effects or resolution loss with depth.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Chemically Specific Coherent Diffractive Imaging of Reactions at Buried Interfaces with Few Nanometer Precision

et al. 2016

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Characterizing buried layers and interfaces is critical for a host of applications in nanoscience and nano-manufacturing. Here we demonstrate non-invasive, non-destructive imaging of buried interfaces using a tabletop, extreme ultraviolet (EUV), coherent diffractive imaging (CDI) nanoscope. Copper nanostructures inlaid in SiO2 are coated with 100 nm of aluminum, which is opaque to visible light and thick enough that neither optical microscopy nor atomic force microscopy can image the buried interfaces. Short wavelength (29 nm) high harmonic light can penetrate the aluminum layer, yielding high-contrast images of the buried structures. Moreover, differences in the absolute reflectivity of the interfaces before and after coating reveal the formation of interstitial diffusion and oxidation layers at the Al-Cu and Al-SiO2 boundaries. Finally, we show that EUV CDI provides a unique capability for quantitative, chemically-specific imaging of buried structures, and the material evolution that occurs at these buried interfaces, compared with all other approaches.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Chemically Specific Coherent Diffractive Imaging of Reactions at Buried Interfaces with Few Nanometer Precision

et al. 2016

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“…The practicality of directly determining three-dimensional structures of noncrystalline biological materials using x-ray diffraction patterns is still being assessed (34). FIGURE 7 Comparison between observed equatorial intensity profiles and those calculated from the best-fit models.…”

Section: Comparison With Other Techniquesmentioning

confidence: 99%

X-Ray Fiber Diffraction Recordings from Oriented Demembranated Chlamydomonas Flagellar Axonemes

Toba

Iwamoto

Kamimura

et al. 2015

Biophysical Journal

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The high homology of its axonemal components with humans and a large repertoire of axonemal mutants make Chlamydomonas a useful model system for experiments on the structure and function of eukaryotic cilia and flagella. Using this organism, we explored the spatial arrangement of axonemal components under physiological conditions by small-angle x-ray fiber diffraction. Axonemes were oriented in physiological solution by continuous shear flow and exposed to intense and stable x rays generated in the synchrotron radiation facility SPring-8, BL45XU. We compared diffraction patterns from axonemes isolated from wild-type and mutant strains lacking the whole outer arm (oda1), radial spoke (pf14), central apparatus (pf18), or the α-chain of the outer arm dynein (oda11). Diffraction of the axonemes showed a series of well-defined meridional/layer-line and equatorial reflections. Diffraction patterns from mutant axonemes exhibited a systematic loss/attenuation of meridional/layer-line reflections, making it possible to determine the origin of various reflections. The 1/24 and 1/12 nm(-1) meridional reflections of oda1 and oda11 were much weaker than those of the wild-type, suggesting that the outer dynein arms are the main contributor to these reflections. The weaker 1/32 and 1/13.7 nm(-1) meridional reflections from pf14 compared with the wild-type suggest that these reflections come mainly from the radial spokes. The limited contribution of the central pair apparatus to the diffraction patterns was confirmed by the similarity between the patterns of the wild-type and pf18. The equatorial reflections were complex, but a comparison with electron micrograph-based models allowed the density of each axonemal component to be estimated. Addition of ATP to rigor-state axonemes also resulted in subtle changes in equatorial intensity profiles, which could report nucleotide-dependent structural changes of the dynein arms. The first detailed description of axonemal reflections presented here serves as a landmark for further x-ray diffraction studies to monitor the action of constituent proteins in functional axonemes.

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超快极紫外光源产生及其在半导体检测中的应用（特邀）

Zeng Zhinan

2024

光学学报

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Lensless coherent imaging of proteins and supramolecular assemblies: Efficient phase retrieval by the charge flipping algorithm

Cited by 3 publications

References 73 publications

Quantitative Chemically Specific Coherent Diffractive Imaging of Reactions at Buried Interfaces with Few Nanometer Precision

Quantitative Chemically Specific Coherent Diffractive Imaging of Reactions at Buried Interfaces with Few Nanometer Precision

X-Ray Fiber Diffraction Recordings from Oriented Demembranated Chlamydomonas Flagellar Axonemes

超快极紫外光源产生及其在半导体检测中的应用（特邀）

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