Lentiavidins: Novel avidin-like proteins with low isoelectric points from shiitake mushroom (Lentinula edodes)

Takakura, Yoshimitsu; Sofuku, Kozue; Tsunashima, Masako; Kuwata, Shigeru

doi:10.1016/j.jbiosc.2015.09.003

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…This is in accordance with the results of our study, which showed that 29 genes of a total of 40 BpGRASs were intronless ( Supplementary Table 3 ). Moreover, the GRAVY index and a pI value of less than 7 in a majority of the GRAS proteins in Table S3 indicated that the GRAS group might be involved in protein-protein interactions ( Jing et al., 2017 ) that were very specific to GRAS proteins, because proteins with low pI values tend to minimize the chances of non-specific interactions with nucleic acids and other acidic proteins ( Takakura et al., 2015 ). Chromosomal position showed that the identified 40 BpGRASs were distributed on the 14 chromosomes of birch unevenly except BpGRAS2 ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, expression and salt stress tolerance analysis of the GRAS transcription factor family in Betula platyphylla

Tian

Zhang

et al. 2022

Front. Plant Sci.

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The GRAS gene family is a plant-specific family of transcription factors and play a vital role in many plant growth processes and abiotic stress responses. Nevertheless, the functions of the GRAS gene family in woody plants, especially in Betula platyphylla (birch), are hardly known. In this study, we performed a genome-wide analysis of 40 BpGRAS genes (BpGRASs) and identified typical GRAS domains of most BpGRASs. The BpGRASs were unevenly distributed on 14 chromosomes of birch and the phylogenetic analysis of six species facilitated the clustering of 265 GRAS proteins into 17 subfamilies. We observed that closely related GRAS homologs had similar conserved motifs according to motif analysis. Besides, an analysis of the expression patterns of 26 BpGRASs showed that most BpGRASs were highly expressed in the leaves and responded to salt stress. Six BpGRASs were selected for cis-acting element analysis because of their significant upregulation under salt treatment, indicating that many elements were involved in the response to abiotic stress. This result further confirmed that these BpGRASs might participate in response to abiotic stress. Transiently transfected birch plants with transiently overexpressed 6 BpGRASs and RNAi-silenced 6 BpGRASs were generated for gain- and loss-of-function analysis, respectively. In addition, overexpression of BpGRAS34 showed phenotype resistant to salt stress, decreased the cell death and enhanced the reactive oxygen species (ROS) scavenging capabilities and proline content under salt treatment, consistent with the results in transiently transformed birch plants. This study is a systematic analysis of the GRAS gene family in birch plants, and the results provide insight into the molecular mechanism of the GRAS gene family responding to abiotic stress in birch plants.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification, expression and salt stress tolerance analysis of the GRAS transcription factor family in Betula platyphylla

Tian

Zhang

et al. 2022

Front. Plant Sci.

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“…Since the RPs are involved mostly in interactions that make up the ribosome complex along with rRNAs, the RPs are expected to have similar isoelectric pH range to facilitate their molecular interactions. Proteins with high isoelectric points tend to interact with DNA, RNA and other biomolecules that are negatively charged whereas the proteins with low isoelectric points prevent non-specific interaction with nucleic acids (Takakura et al, 2016 ). In a recent study, RPS6 has been reported to interact with a plant specific histone-deacetylase-2B and also with nucleosome assembly protein-1 thereby regulating the transcription of r-DNA in Arabidopsis (Son et al, 2015 ; Xiong and Sheen, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Comprehensive Expression Profiling of Ribosomal Protein Small Subunit (RPS) Genes and their Comparative Analysis with the Large Subunit (RPL) Genes in Rice

et al. 2017

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Ribosomal proteins (RPs) are indispensable in ribosome biogenesis and protein synthesis, and play a crucial role in diverse developmental processes. Our previous studies on Ribosomal Protein Large subunit (RPL) genes provided insights into their stress responsive roles in rice. In the present study, we have explored the developmental and stress regulated expression patterns of Ribosomal Protein Small (RPS) subunit genes for their differential expression in a spatiotemporal and stress dependent manner. We have also performed an in silico analysis of gene structure, cis-elements in upstream regulatory regions, protein properties and phylogeny. Expression studies of the 34 RPS genes in 13 different tissues of rice covering major growth and developmental stages revealed that their expression was substantially elevated, mostly in shoots and leaves indicating their possible involvement in the development of vegetative organs. The majority of the RPS genes have manifested significant expression under all abiotic stress treatments with ABA, PEG, NaCl, and H2O2. Infection with important rice pathogens, Xanthomonas oryzae pv. oryzae (Xoo) and Rhizoctonia solani also induced the up-regulation of several of the RPS genes. RPS4, 13a, 18a, and 4a have shown higher transcript levels under all the abiotic stresses, whereas, RPS4 is up-regulated in both the biotic stress treatments. The information obtained from the present investigation would be useful in appreciating the possible stress-regulatory attributes of the genes coding for rice ribosomal small subunit proteins apart from their functions as house-keeping proteins. A detailed functional analysis of independent genes is required to study their roles in stress tolerance and generating stress- tolerant crops.

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“…Bacterial AVI analogs are SAV-isolated from Streptomyces avidinii [ 36 ], and rhizavidin from the root nodule nitrogen-fixing bacteria Rhizobium etli [ 37 ]. Tamavidins and Lentiavidins are other representatives derived from edible Tamogitake (Pleurotus citrinopileatus) [ 38 ] and Shiitake mushrooms ( Lentinula edodes ) [ 39 ]. All of these biotin-binding proteins and numerous recombinant products have moderate to extremely high affinity for biotin (Kd = 10 −7 –10 −16 M); therefore, they can interfere with the “SAV/AVI–B 7 ” interaction when present in a sample.…”

Section: Resultsmentioning

confidence: 99%

Influence of Endogenous Factors of Food Matrices on Avidin—Biotin Immunoassays for the Detection of Bacitracin and Colistin in Food

Burkin

Galvidis

Eremin

2022

Foods

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(Strept)avidin–biotin technology is frequently used in immunoassay systems to improve their analytical properties. It is known from clinical practice that many (strept)avidin–biotin-based tests provide false results when analyzing patient samples with a high content of endogenous biotin. No specific investigation has been carried out regarding possible interferences from avidin (AVI) and biotin (B7) contained in food matrices in (strept)avidin–biotin-based immunoanalytical systems for food safety. Two kinds of competitive ELISAs for bacitracin (BT) and colistin (COL) determination in food matrices were developed based on conventional hapten–protein coating conjugates and biotinylated BT and COL bound to immobilized streptavidin (SAV). Coating SAV–B7–BT and SAV–B7–COL complexes-based ELISAs provided 2- and 15-times better sensitivity in BT and COL determination, corresponding to 0.6 and 0.3 ng/mL, respectively. Simultaneously with the determination of the main analytes, these kinds of tests were used as competitive assays for the assessment of AVI or B7 content up to 10 and 1 ng/mL, respectively, in food matrices (egg, infant milk formulas enriched with B7, chicken and beef liver). Matrix-free experiments with AVI/B7-enriched solutions showed distortion of the standard curves, indicating that these ingredients interfere with the adequate quantification of analytes. Summarizing the experience of the present study, it is recommended to avoid immunoassays based on avidin–biotin interactions when analyzing biosamples containing these endogenous factors or enriched with B7.

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Lentiavidins: Novel avidin-like proteins with low isoelectric points from shiitake mushroom (Lentinula edodes)

Cited by 13 publications

References 27 publications

Genome-wide identification, expression and salt stress tolerance analysis of the GRAS transcription factor family in Betula platyphylla

Genome-wide identification, expression and salt stress tolerance analysis of the GRAS transcription factor family in Betula platyphylla

Genome-Wide Identification and Comprehensive Expression Profiling of Ribosomal Protein Small Subunit (RPS) Genes and their Comparative Analysis with the Large Subunit (RPL) Genes in Rice

Influence of Endogenous Factors of Food Matrices on Avidin—Biotin Immunoassays for the Detection of Bacitracin and Colistin in Food

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