Lentivirus-mediated RNAi silencing targeting ABCC2 increasing the sensitivity of a human nasopharyngeal carcinoma cell line against cisplatin

Xie, Si Ming; Fang, Wei; Liu, Zhen; Wang, Shuang Xi; Li, Xin; Liu, Teng Fei; Xie, Wei; Yao, Kai

doi:10.1186/1479-5876-6-55

Cited by 32 publications

(20 citation statements)

References 17 publications

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“…In clinical practice, NPC is treated by radiation, but the therapeutic effect is not satisfactory (8)(9)(10). In recent years, many investigations have focused on extracting active ingredients from natural plants to prevent and treat cancer and to investigate their anti-cancer mechanism (11,12).…”

Section: Introductionmentioning

confidence: 99%

Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells

Feng-lian

2010

Braz J Med Biol Res

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Section: Introductionmentioning

confidence: 99%

Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells

Feng-lian

2010

Braz J Med Biol Res

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“…cDNA was synthesized using RT reagents (Takara, Dalian, China) from 1 mg of total RNA using oligo(dT) 15 in 20 ml reaction volume. Real-time PCR was used to measure the expression of MMP2 mRNA using SYBR Pre-mix Ex Taq according to the manufacturer's protocol (Takara) with an Mx3000P real-time PCR system (Stratagene, La Jolla, USA) as described previously [18]. The sequence for sense primer was 5 0 -ACCAGCTGGCCTAGTGATGATG-3 0 , and for anti-sense primer was 5 0 -GGCTTCCGCATGGTCTCG ATG-3 0 .…”

Section: Sample Collectionmentioning

confidence: 99%

Matrix metalloproteinase 2 promotes cell growth and invasion in colorectal cancer

Dong¹,

Hong²,

Zhang³

et al. 2011

ABBS

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Colorectal cancer (CRC) is the third leading cause of cancer-related death in the western world. In this study, we evaluated the expression of matrix metalloproteinase 2 gene (MMP2) in CRC and analyzed its correlation with clinicopathological features. We found that the expression of MMP2 was significantly higher in CRC tissues than in the colorectal tissues. In addition, high levels of MMP2 protein were positively correlated with the status of tumor size, lymph node metastasis, distant metastasis, Dukes' stage, and tumor invasion. Moreover, patients with higher MMP2 levels had markedly shorter overall survivals than those with low MMP2 levels. Multivariate analysis results suggested that the level of MMP2 expression is an independent prognostic indicator for the survival of patients with CRC. Silencing MMP2 expression in CRC cell lines with lentiviral-mediated shRNA markedly suppressed cell proliferation, colony formation, and invasion. Furthermore, we observed that vascular endothelial growth factor (VEGF) and membrane type 1 (MT1)-MMP protein levels were decreased in MMP2-down-regulated colorectal cells. Therefore, our study demonstrated that MMP2 is an important factor related to carcinogenesis and metastasis of CRC, and MMP2 promotes CRC cell growth and invasion by up-regulating VEGF and MT1-MMP expression, which makes this pathway a potential target for cancer treatment.

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“…The combination of radiotherapy and adjuvant chemotherapy is the prevalent treatment of NPC, and the most chemotherapeutic agents used for treating NPC are cisplatin based [18]. Nevertheless, the acute and late toxicities, as well as resistance of the chemotherapies have significantly inscreased [19,20]. Therefore, there is a need for the development of less toxic and more effective agents for NPC treatment.…”

mentioning

confidence: 99%

New Steroidal Saponins and Sterol Glycosides from Paris polyphylla var. yunnanensis

Wang

et al. 2012

Planta Med

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Four new steroidal saponins, pariposides A-D (1-4), and two new sterol glycosides, pariposides E-F (5-6), along with eight known steroidal saponins (7-14), two known sterol glycosides (15-16), and two known ecdysteroids (17-18), were isolated from the roots of Paris polyphylla var. yunnanensis. Among them, compounds 1-4 are the first spirostanol saponins with a peroxy group located between C-5 and C-8 of the aglycone. Their structures were determined by detailed spectroscopic analyses and chemical methods. All the isolated compounds were evaluated for their in vitro cytotoxicities against human nasopharyngeal carcinoma epithelial (CNE) cells, and steroidal saponins 7, 11, 13, and 14 showed a potent antiproliferative effect on CNE cells with IC₅₀ values of 9.2, 4.7, 11.1, and 2.7 µM, respectively.

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Lentivirus-mediated RNAi silencing targeting ABCC2 increasing the sensitivity of a human nasopharyngeal carcinoma cell line against cisplatin

Cited by 32 publications

References 17 publications

Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells

Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells

Matrix metalloproteinase 2 promotes cell growth and invasion in colorectal cancer

New Steroidal Saponins and Sterol Glycosides from Paris polyphylla var. yunnanensis

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