LEP 3′HVR is associated with obesity and leptin levels in Brazilian individuals

Hinuy, Hamilton Massayuki; Hirata, Mário Hiroyuki; Sampaio, Marcelo Ferraz; Armaganijan, Dikran; Salazar, Luis A.; Hirata, Rosário Dominguez Crespo

doi:10.1016/j.ymgme.2006.04.012

Cited by 11 publications

(12 citation statements)

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“…LEP 3´HVR alleles were grouped in class I (short) and class II (long) as previously described (11 Anthropometric, demographic and biochemical data of the studied group are shown in table 1. Four women refused to inform their ages.…”

Section: Discussionmentioning

confidence: 99%

“…The LEP 3´HVR is a (TTTC) n tetranucleotide repeat located at the 3' end of the LEP that has been associated with increased risk for obesity and high plasma leptin levels as well (10)(11). These effects may be due to the linkage of the 3'HVR with -2548G>A variant.…”

Section: Discussionmentioning

confidence: 99%

“…LEP -2548G>A and 3´HVR polymorphisms were genotyped by polymerase chain reaction (PCR) and fragment analysis as previously described (8,11). The accuracy of the genotyping was evaluated by performing duplicate analysis of 20% samples randomly selected.…”

Section: Dna Genotypingmentioning

confidence: 99%

“…Microsatellite markers such as the highly variable tetranucleotide repeat (TTTC) n located in the 3'-flanking region of the LEP (3'HVR) has also been associated with obesity-related traits and leptin plasma levels (6,10,11). However, the relation of this variant with other polymorphisms in the LEP gene on metabolic and obesity-related characteristics remains to be investigated.…”

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confidence: 99%

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Relationship between variants of the leptin gene and obesity and metabolic biomarkers in Brazilian individuals

Hinuy¹,

Hirata²,

Sampaio³

et al. 2010

Arq Bras Endocrinol Metab

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Objective:The relationship between variants of the leptin gene (LEP) and obesity and metabolic biomarkers was investigated in Brazilian individuals. Subjects and methods: One-hundred-ten obese (BMI > 30 kg/m 2 ) and 100 non-obese individuals (145 women and 65 men, aged 49 ± 14 years) were randomly selected. Plasma leptin, glycemia, serum lipid measurements and LEP -2548G>A and 3'HVR polymorphisms were analyzed. Results: The LEP -2548GG genotype was associated with a 2.2% and 2.0% increase in BMI (p = 0.009) and plasma leptin (p = 0.031), respectively. 3'HVR I/II (classes I/I+I/II) genotypes contributed with 1.8% of BMI values (p = 0.046). LEP I/G combined genotypes (I/IGG, I/IGA and I/IIGG) were associated with obesity, and increased BMI, waist circumference, leptin and triglycerides (p < 0.05). These relationships were found in women (p < 0.05) but not in men. LEP I/G combined genotypes were not associated with hypertension, hyperglycemia, dyslipidemia and coronary artery disease. Conclusions: LEP I/G combined genotypes are associated with obesity-related metabolic biomarkers and phenotype in a gender-dependent manner. Arq Bras Endocrinol Metab. 2010;54(3):282-8 Keywords Leptin; gene polymorphism; obesity; metabolic biomarkers; plasma leptin rEsUMO Objetivo: A relação entre as variantes do gene da leptina (LEP) e obesidade e biomarcadores metabólicos foi investigada em indivíduos brasileiros. Sujeitos e métodos: Cento e dez indiví-duos obesos (IMC > 30 kg/m 2 ) e 100 não obesos (145 mulheres e 65 homens, idade 49 ± 14 anos) foram selecionados aleatoriamente. Leptina plasmática, glicemia, lípides séricos e polimorfismos LEP -2548G>A e 3'HVR foram analisados. Resultados: O genótipo -2548GG foi associado com aumento de 2,2% e 2,0% no IMC (p = 0,009) e leptina plasmática (p = 0,031), respectivamente, enquanto os genótipos 3´HVR I/II (classes I/I+I/II) contribuíram com 1,8% dos valores de IMC (p = 0,046). Os genótipos combinados LEP I/G (I/IGG, I/IGA e I/IIGG) foram associados com obesidade e IMC aumentado, circunferência abdominal, leptina e triglicérides aumentados (p < 0,05). Essas relações foram encontradas em mulheres (p < 0,05), mas não em homens. Os genótipos LEP I/G combinados não foram associados com hipertensão, hiperglicemia, dislipidemia e doença arterial coronariana. Conclusões: Genótipos combinados LEP I/G são associados com biomarcadores metabólicos e fenótipo de obesidade de forma gênero-dependente. Arq Bras Endocrinol Metab. 2010;54(3):282-8 Descritores Leptina; polimorfismo genético; obesidade; biomarcadores metabólicos; leptina plasmática

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Dna Genotypingmentioning

confidence: 99%

mentioning

confidence: 99%

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Relationship between variants of the leptin gene and obesity and metabolic biomarkers in Brazilian individuals

Hinuy¹,

Hirata²,

Sampaio³

et al. 2010

Arq Bras Endocrinol Metab

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“…STRs located near the leptin-melaconortin pathway genes have been also found to be linked with obesity. We and other investigators have reported that a highly variable tetranucleotide repeat located at the 3'-flanking region of the LEP (3'HVR) is associated with obesity-related traits and leptinemia (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 96%

Relationship of short tandem repeats flanking leptin-melanocortin pathway genes with anthropometric profile and leptinemia in Brazilian individuals

Hinuy

Arazi

Hirata

et al. 2012

Arq Bras Endocrinol Metab

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Objective: To investigate the relationship of short tandem repeats (STR) near genes involved in the leptin-melanocortin pathway with body mass index (BMI) and leptinemia. Subjects and methods: Anthropometric variables and leptinemia were measured in 100 obese and 110 nonobese individuals. D1S200, D2S1788, DS11912, and D18S858 loci were analyzed by PCR and high-resolution electrophoresis. Results: Overall STR allele frequencies were similar between the obese and non-obese group (p > 0.05). Individual alleles D1S200 (17), D11S912 (43), D18S858 (11/12) were associated with obesity (p < 0.05). Individuals carrying these alleles showed higher BMI than non-carriers (p < 0.05). Moreover, a relationship between D18S858 11/12 alleles and increased waist circumference was found (p = 0.040). On the other hand, leptinemia was not influenced by the studied STRs (p > 0.05). Conclusions: D1S200, D11S912, and D18S858 loci are associated with increased BMI and risk for obesity in this sample. Arq Bras Endocrinol Metab. 2012;56(1):47-53Keywords STR; obesity; body mass index; letpin RESUMO Objetivo: Investigar a relação de short tandem repeats (STR) em genes envolvidos na via da leptina-melanocortina com índice de massa corporal (IMC) e leptinemia. Sujeitos e métodos: Variáveis antropométricas e leptinemia foram medidas em 100 indivíduos obesos e 110 não obesos. Os loci D1S200, D2S1788, DS11912 e D18S858 foram analisados por PCR e eletroforese de alta resolução. Resultados: As frequências globais dos alelos da STR foram similares entre os grupos obeso e não obeso (p > 0,05). Alelos individuais de D1S200 (17), D11S912 (43), D18S858 (11/12) foram associados com obesidade (p < 0,05). Indivíduos portadores desses alelos apresentaram valores de IMC maiores que os dos não portadores (p < 0,05). Além disso, a presença dos alelos D18S858 11/12 foi relacionada com circunferência abdominal elevada (p = 0,040). Por outro lado, a leptinemia não foi influenciada pelos STRs estudados (p > 0,05). Conclusões: Os loci D1S200, D11S912 e D18S858 são associados com IMC aumentado e risco de obesidade nesta amostra populacional. Arq Bras Endocrinol Metab. 2012;56(1):47-53 Descritores STR; obesidade; índice de massa corporal; leptina

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