Lepraric acid derivatives as chemotaxonomic markers in Hypoxylon aeruginosum, Chlorostroma subcubisporum and C. cyaninum, sp. nov.

Læssøe, Thomas; Srikitikulchai, Prasert; Fournier, Jacques; Köpcke, Bärbel; Stadler, Marc

doi:10.1016/j.funbio.2010.03.010

Cited by 30 publications

(16 citation statements)

References 17 publications

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“…DNA was extracted from mycelial cultures using protocols and genomic DNA extraction kits described in Laessøe et al (2010), Kuhnert et al (2014a), and Otto et al (2016). Partial sequences of four DNA loci were used as phylogenetic markers, namely a) internal transcribed spacer region (ITS); b) 28S large subunit (LSU) of ribosomal DNA; c) second largest subunit of the DNA-directed RNA polymerase II (RPB2); and d) beta-tubulin (TUB2) were generated using standard primers introduced by White et al (1990;ITS4), Gardes and Bruns (1993;ITS1F), O'Donnell (1993;NL4), Vilgalys and Hester (1990;LR07 and LR0R), Liu et al (1999;RPB2-5F, -7cF, and -7cR), and O'Donnell and Cigelnik (1997; T1, T2, T11, T21, T22, T121, T222, and T224), Glass and Donaldson (1995;Bt2b), and protocols as described by Laessøe et al (2010) and Otto et al (2016).…”

Section: Molecular Phylogenetic Analysesmentioning

confidence: 99%

“…Partial sequences of four DNA loci were used as phylogenetic markers, namely a) internal transcribed spacer region (ITS); b) 28S large subunit (LSU) of ribosomal DNA; c) second largest subunit of the DNA-directed RNA polymerase II (RPB2); and d) beta-tubulin (TUB2) were generated using standard primers introduced by White et al (1990;ITS4), Gardes and Bruns (1993;ITS1F), O'Donnell (1993;NL4), Vilgalys and Hester (1990;LR07 and LR0R), Liu et al (1999;RPB2-5F, -7cF, and -7cR), and O'Donnell and Cigelnik (1997; T1, T2, T11, T21, T22, T121, T222, and T224), Glass and Donaldson (1995;Bt2b), and protocols as described by Laessøe et al (2010) and Otto et al (2016). PCR products were also purified and sequenced as described in Laessøe et al (2010) and Otto et al (2016). Sequences were analyzed and processed in Geneious® 7.1.9 (http://www.geneious.com, Kearse et al 2012).…”

Section: Molecular Phylogenetic Analysesmentioning

confidence: 99%

“…However, all genera formerly assigned to the Xylariaceae with cleistocarpous asci like Phylacia, Rhopalostroma and Thamnomyces belong to the Hypoxylaceae in the current sense. Chlorostroma is accommodated in the Hypoxylaceae due to its similarity to Hypoxylon aeruginosum and the highly similar secondary metabolite profiles (Laessøe et al 2010).…”

Section: General Description Of the Hypoxylaceaementioning

confidence: 99%

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Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales

et al. 2017

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A multigene phylogeny was constructed, including a significant number of representative species of the main lineages in the Xylariaceae and four DNA loci the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, the second largest subunit of the RNA polymerase II (RPB2), and beta-tubulin (TUB2). Specimens were selected based on more than a decade of intensive morphological and chemotaxonomic work, and cautious taxon sampling was performed to cover the major lineages of the Xylariaceae; however, with emphasis on hypoxyloid species. The comprehensive phylogenetic analysis revealed a clear-cut segregation of the Xylariaceae into several major clades, which was well in accordance with previously established morphological and chemotaxonomic concepts. One of these clades contained Annulohypoxylon, Hypoxylon, Daldinia, and other related genera that have stromatal pigments and a nodulisporium-like anamorph. They are accommodated in the family Hypoxylaceae, which is resurrected and emended. Representatives of genera with a nodulisporium-like anamorph and bipartite stromata, lacking stromatal pigments (i.e. Biscogniauxia, Camillea, and Obolarina) appeared in a clade basal to the xylarioid taxa. As they clustered with Graphostroma platystomum, they are accommodated in the Graphostromataceae. The new genus Jackrogersella with J. multiformis as type species is segregated from Annulohypoxylon. The genus Pyrenopolyporus is resurrected for Hypoxylon polyporus and allied species. The genus Daldinia and its allies Entonaema, Rhopalostroma, Ruwenzoria, and Thamnomyces appeared in two separate subclades, which may warrant further splitting of Daldinia in the future, and even Hypoxylon was divided in several clades. However, more species of these genera need to be studied before a conclusive taxonomic rearrangement can be envisaged. Epitypes were designated for several important species in which living cultures and molecular data are available, in order to stabilise the taxonomy of the Xylariales.

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Section: Molecular Phylogenetic Analysesmentioning

confidence: 99%

Section: Molecular Phylogenetic Analysesmentioning

confidence: 99%

Section: General Description Of the Hypoxylaceaementioning

confidence: 99%

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Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales

et al. 2017

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“…Paecilomyces viridis was concurrently transferred to the genus as Chamaeleomyces viridis (Segretain et al) Sigler (Sigler et al 2010). Laessøe et al (2010). However, the taxonomic conclusion is not yet established because cultures to study the asexual morphology, secondary metabolites and molecular phylogeny are lacking (Laessøe et al 2010).…”

Section: Fungal Diversitymentioning

confidence: 99%

Towards a natural classification and backbone tree for Sordariomycetes

et al. 2015

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Sordariomycetes is one of the largest classes of Ascomycota and is characterised by perithecial ascomata and inoperculate unitunicate asci. The class includes many important plant pathogens, as well as endophytes, saprobes, epiphytes, and fungicolous, lichenized or lichenicolous taxa. The class includes freshwater, marine and terrestrial taxa and has a worldwide distribution. This paper provides an updated outline of the Sordariomycetes and a backbone tree incorporating asexual and sexual genera in the class. Based on phylogeny and morphology we introduced three subclasses; D i a p o r t h o m y c e t i d a e , L u l w o r t h i o m y c e t i d a e a n dTogniniales. The outline is based on literature to the end of 2014 and the backbone tree published in this paper. Notes for 397 taxa with information, such as new family and genera novelties, novel molecular data published since the Outline of Ascomycota 2009, and new links between sexual and asexual genera and thus synonymies, are provided. The Sordariomycetes now comprises six subclasses, 28 orders, 90 families and 1344 genera. In addition a list of 829 genera with uncertain placement in Sordariomycetes is also provided.

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“…This allowed for identification of its specific metabolites (carneic acids A and B) after extensive preparative chromatography of the stromatal extracts and subsequent structure elucidation of the purified metabolites by spectral methods. On the other hand, the identification of the major stromatal metabolites of H. aeruginosum and the similar species of Chlorostroma as lepraric acids and their derivatives was accomplished due to the availability of standards that were originally isolated from lichens (Laessøe et al 2010). The same was accomplished by Fournier et al (2010), who found lecanoric acids, which are widely distributed in lichenised ascomycetes, for the first time as apparently species-specific major stromatal metabolites of H. addis.…”

Section: Introductionmentioning

confidence: 99%

Hypoxyvermelhotins A–C, new pigments from Hypoxylon lechatii sp. nov

Kuhnert

Heitkämper

Fournier³

et al. 2014

Fungal Biology

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JournalFungal biology ABSTRACTA new species of Hypoxylon was discovered, based on material collected in the Caribbean and French Guiana and recognised on the basis of new combination of morpholological characters in comparison with type and authentic material of macroscopically similar taxa. These findings were corroborated by the rather isolated positions of its ITS-nrDNA and beta-tubulin DNA sequences in molecular phylogenies. However, the most salient feature of this fungus only became evident by a comparison of its stromatal HPLC profile, revealing several secondary metabolites that were hitherto not observed in stromata of any other member of the Xylariaceae. Part of the stromata were subsequently extracted to isolate these apparently specific components, using preparative chromatography. Five metabolites were obtained in pure state, and their chemical structures were elucidated by means of high resolution mass spectrometry and nuclear magnetic resonance spectroscopy. They turned out to be tetramic acid derivatives of the so-called vermelhotin type. Aside from vermelhotin, previously isolated from cultures of endophytic fungi, we identified three novel congeners, for which the trivial names hypoxyvermelhotins A-C were proposed. Like vermelhotin, they constitute red pigments and a preliminary biological characterisation revealed them to have rather strong cytotoxic and moderate to weak antimicrobial effects. These results further illustrate the high diversity of unique secondary metabolites in stromata of the hypoxyloid Xylariaceae, a family in which biological diversity seems to parallel the chemical diversity of their bioactive principles to a great extent.2

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Lepraric acid derivatives as chemotaxonomic markers in Hypoxylon aeruginosum, Chlorostroma subcubisporum and C. cyaninum, sp. nov.

Cited by 30 publications

References 17 publications

Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales

Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales

Towards a natural classification and backbone tree for Sordariomycetes

Hypoxyvermelhotins A–C, new pigments from Hypoxylon lechatii sp. nov

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