Leptin and Obesity in Ovarian Dysfunction in Menopause

Diel, Patrick; Weigt, Carmen

doi:10.1007/978-1-62703-373-2_19

Cited by 2 publications

(2 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…every 4 weeks in order to achieve pharmacological ovariectomy [ 11 ]. After a 6-week resting period to verify the surgically or pharmacologically induced menopause and to ensure that all animals were killed at the same stage of the estrus phase (all the control rats were in the proestrus phase) via Giemsa staining method we checked the estrogen level with estrogen quantitative enzyme-linked immunosorbent assay according to the manufacturer's directions (Quantikine rat Estrogen Elisa kit, R&D Systems Inc.) [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Endogenous Estrogen-Mediated Heme Oxygenase Regulation in Experimental Menopause

Pósa

Szabó

Csonka

et al. 2015

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Estrogen deficiency is one of the main causes of age-associated diseases in the cardiovascular system. Female Wistar rats were divided into four experimental groups: pharmacologically ovariectomized, surgically ovariectomized, and 24-month-old intact aging animals were compared with a control group. The activity and expression of heme oxygenases (HO) in the cardiac left ventricle, the concentrations of cardiac interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), the myeloperoxidase (MPO) activity in the cardiac left ventricle, and the effects of heme oxygenase blockade (by 24-hour and 1-hour pretreatment with tin-protoporphyrin IX, SnPP) on the epinephrine and phentolamine-induced electrocardiogram ST segment changes in vivo were investigated. The cardiac HO activity and the expression of HO-1 and HO-2 were significantly decreased in the aged rats and after ovariectomy. Estrogen depletion was accompanied by significant increases in the expression of IL-6 and TNF-α. The aged and ovariectomized animals exhibited a significantly elevated MPO activity and a significant ST segment depression. After pretreatment with SnPP augmented ST segment changes were determined. These findings demonstrate that the sensitivity to cardiac ischemia in estrogen depletion models is associated with suppression of the activity and expression of the HO system and increases in the secretion of proinflammatory cytokines and biomarkers.

show abstract

Section: Methodsmentioning

confidence: 99%

Endogenous Estrogen-Mediated Heme Oxygenase Regulation in Experimental Menopause

Pósa

Szabó

Csonka

et al. 2015

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…The 12 : 12 h light-dark cycle started at 6:00 AM, and the room temperature was maintained at 20–23°C. All OVX rats were in the proestrus stage, which is characterized by the unique presence of nucleated epithelial cells stained with a 0.1% Giemsa solution and observed under light microscopy (×100) [30]. …”

Section: Methodsmentioning

confidence: 99%

Sexual Dimorphism of Cardiovascular Ischemia Susceptibility Is Mediated by Heme Oxygenase

Pósa

Kupai

Ménesi

et al. 2013

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

We investigated the gender differences in heme-oxygenase (HO) enzyme, which produces endogenous vascular protective carbon monoxide (CO). We studied (1) the activity and expression of HO enzymes in the left ventricle (LV) and aorta, (2) basal increase in basal blood pressure provoked by arginine vasopressine (AVP) in vivo, (3) the heart perfusion induced by AVP, (4) the ST segment depression provoked by adrenaline and 30 seconds later phentolamine, and (5) the aorta ring contraction induced by AVP in female and male Wistar rats. We found that HO activity and the expression of HO-1 and HO-2 were increased in female rat aorta and LV. We demonstrated that the basal blood pressure and administration of AVP provoked blood pressure response are increased in the males; the female myocardium was less sensitive towards angina. Both differences could be aggravated by the inhibition of HO. The aorta rings were more susceptible towards vasoconstriction by AVP in males; isolated heart perfusion decrease was higher in males. The HO inhibition aggravated the heart perfusion in both sexes. In conclusion, the increased HO activity and expression in females might play a role in the sexual dimorphism of cardiovascular ischemia susceptibility during the reproductive age.

show abstract

Leptin and Obesity in Ovarian Dysfunction in Menopause

Cited by 2 publications

References 77 publications

Endogenous Estrogen-Mediated Heme Oxygenase Regulation in Experimental Menopause

Endogenous Estrogen-Mediated Heme Oxygenase Regulation in Experimental Menopause

Sexual Dimorphism of Cardiovascular Ischemia Susceptibility Is Mediated by Heme Oxygenase

Contact Info

Product

Resources

About