Leptin receptor and fatty acid desaturase-2 gene variants affect fat, color and production profile of dry-cured hams

Suárez-Mesa, Rafael; Ros‐Freixedes, Roger; Tör, M.; Reixach, Josep Peñuelas i; Pena, Ramona N.; Estany, J.

doi:10.1016/j.meatsci.2020.108399

Cited by 6 publications

(8 citation statements)

References 33 publications

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“…In particular, these authors found that the A allele was associated to a lighter pork color (greater CIE L* parameter). Although these authors did not analyze IMF, the lighter color associated to the A allele is compatible with greater levels of IMF (Schwab et al, 2006;Suárez-Mesa et al, 2021), which adds yet another layer of evidence from an independent population in support of this variant.…”

Section: Preselection Of Candidate Variants In the Agpat Gene Familymentioning

confidence: 90%

Identification of a missense variant in the porcine AGPAT gene family that affects intramuscular fat content through whole-genome sequencing

Molinero

Pena

Estany

et al. 2022

Preprint

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The 1-acylglycerol-3-phosphate O-acyltransferases (AGPAT) are enzymes that catalyze the conversion of lysophosphatidic acid to phosphatidic acid, which is a precursor of triacylglycerol, the main fat reservoir in mammals. In pigs, there are 5 annotated genes in the AGPAT gene family. We used whole-genome sequencing to identify genetic variants in the AGPAT gene family in pigs and then we validated their association with fat content and fatty acid composition. Among the 6,639 variants identified across the AGPAT gene family through whole-genome sequencing of 205 Duroc pigs, we preselected a missense single nucleotide polymorphism in exon 6 of AGPAT5 (rs196952262, A>G) based on minor allele frequency, association analyses, and linkage disequilibrium. The effect of this variant was validated in 1,105 pigs from the same Duroc line genotyped using a high-resolution melt protocol. The A allele showed a positive additive effect for intramuscular fat (+1.12% ± 0.21, p < 0.001, for gluteus medius and +0.89% ± 0.33, p < 0.01, for longissimus). We also observed significant effects on fatty acid composition that were, at least in part, independent of the increased intramuscular fat content. Accounting for intramuscular fat content, the A allele resulted in more monounsaturated fatty acids (+0.34% ± 0.15, p < 0.05, for longissimus) and a greater monounsaturated/polyunsaturated fatty acids ratio (+0.11 ± 0.04, p < 0.01, for gluteus medius and +0.13 ± 0.05, p < 0.05, for longissimus). Although further studies would be needed before the causality of the variant on intramuscular fat content and fatty acid composition can be confirmed, this variant can be used as a marker in assisted selection for modulating pig fat deposition and fatty acid content.

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Section: Preselection Of Candidate Variants In the Agpat Gene Familymentioning

confidence: 90%

Identification of a missense variant in the porcine AGPAT gene family that affects intramuscular fat content through whole-genome sequencing

Molinero

Pena

Estany

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In particular, these authors found that the A allele was associated with a lighter pork color (greater CIE L * parameter). Although these authors did not analyze the IMF, the lighter color associated with the A allele is compatible with greater levels of IMF (Schwab et al, 2006 ; Suárez‐Mesa et al, 2021 ), which adds another layer of evidence in support of this variant from an independent population.…”

Section: Discussionmentioning

confidence: 99%

Identification of a missense variant in the porcine AGPAT gene family associated with intramuscular fat content through whole‐genome sequencing

et al. 2022

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“…In pigs, it has been shown that the homozygous for the recessive T allele of the missense polymorphism rs709596309 C > T of LEPR 3 display higher levels of circulating leptin and fat deposition 4,5 , a clinical outcome that is compatible with a defective expression of the leptin receptor 6 . The TT pigs not only deposit more subcutaneous and inner fat but also intramuscular fat (IMF), both in raw 5 and dry-cured products 7 . It is generally accepted that IMF has a favorable effect on the sensory attributes of pork 8,9 , although its influence on acceptability depends on the consumer habits [10][11][12] and the type of product.…”

Section: Abbreviationsmentioning

confidence: 99%

“…Left hams were individually traced and dry-cured through a four-step process 7 . First, hams were covered with salt and kept for 10 to 14 days at 2 ºC.…”

Section: Cured Ham Processingmentioning

confidence: 99%

Impact of the leptin receptor gene on pig performance and quality traits

Suárez-Mesa,

Ros-Freixedes,

Pena

et al. 2024

Sci Rep

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The recessive T allele of the missense polymorphism rs709596309 C > T of the leptin receptor gene is associated with intramuscular fat. However, its overall impact on pork production is still partial. In this work, we investigated the all-round effects of the TT genotype on lean growth efficiency and carcass, meat and fat quality using data from an experiment that compared the performance of 48 TT and 48 C– (24 CT and 24 CC) Duroc barrows. The TT pigs were less efficient for lean growth than the C– pigs. Although heavier, their carcasses had less lean content, were shorter and had lighter loins. Apart from increasing marbling and saturated fatty acid content, changes caused by the TT genotype in meat and fat quality are likely not enough to be perceived by consumers. The effect on visual marbling score exceeded that on intramuscular fat content, which suggests a direct influence of the T allele on the pattern of fat distribution in muscle. With current low-protein diets, the T allele is expected to be cost-effective only in niche markets where a very high level of marbling is critical.

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Leptin receptor and fatty acid desaturase-2 gene variants affect fat, color and production profile of dry-cured hams

Cited by 6 publications

References 33 publications

Identification of a missense variant in the porcine AGPAT gene family that affects intramuscular fat content through whole-genome sequencing

Identification of a missense variant in the porcine AGPAT gene family that affects intramuscular fat content through whole-genome sequencing

Identification of a missense variant in the porcine AGPAT gene family associated with intramuscular fat content through whole‐genome sequencing

Impact of the leptin receptor gene on pig performance and quality traits

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