Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus

López, Miguel; Seoane, Luísa M.; García, María del Carmen García; Lago, Francisca; Casanueva, Felipe F.; Señarı́s, Rosa; Diéguez, Carlos

doi:10.1006/bbrc.2000.2245

Cited by 180 publications

(128 citation statements)

References 20 publications

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“…In order to simplify the figure, comparisons between SL and LL groups have been omitted. b NL, SL and LL male rats at 24 days old experimental group) the hypothalamus was dissected as previously described [24,25] for RT-PCR study. All these samples were frozen immediately on dry ice and maintained at −80°C until processed.…”

Section: Methodsmentioning

confidence: 99%

“…In order to ensure that the PCR reaction was performed within a linear range, samples were amplified for 15, 20, 25, 30, 35 and 40 cycles. Kinetics for HPRT and the different isoforms of OB-R have been published elsewhere [24,31,34]. The amplified products were resolved in a 2% agarose gel and quantified by densitometry using a digital imaging system (Molecular Analyst, Biorad, CA, USA) [24,31,32,34].…”

Section: Methodsmentioning

confidence: 99%

“…Kinetics for HPRT and the different isoforms of OB-R have been published elsewhere [24,31,34]. The amplified products were resolved in a 2% agarose gel and quantified by densitometry using a digital imaging system (Molecular Analyst, Biorad, CA, USA) [24,31,32,34]. All PCR products were digested with specific restriction enzymes and hybridised with complementary RNA or DNA probes or sequenced in order to determine their specificity [24,31,34].…”

Section: Methodsmentioning

confidence: 99%

“…The amplified products were resolved in a 2% agarose gel and quantified by densitometry using a digital imaging system (Molecular Analyst, Biorad, CA, USA) [24,31,32,34]. All PCR products were digested with specific restriction enzymes and hybridised with complementary RNA or DNA probes or sequenced in order to determine their specificity [24,31,34]. The mRNA content of the different isoforms of OB-R was analysed in each hypothalamus individually.…”

Section: Methodsmentioning

confidence: 99%

“…All sections were scanned and the specific hybridisation signal was quantified by densitometry using a digital imaging system (Molecular Analyst, Biorad) [24,25,[32][33][34][35][36]. The optical density of the hybridisation signal was determined and subsequently corrected by the optical density of its adjacent background value.…”

Section: Methodsmentioning

confidence: 99%

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A possible role of neuropeptide Y, agouti-related protein and leptin receptor isoforms in hypothalamic programming by perinatal feeding in the rat

et al. 2004

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Aim/hypothesis: Perinatal overfeeding predisposes humans and rats to obesity and diabetes in later life. One classical model for studying the effect of early feeding is manipulation of the size of rat litters. Rats growing up in small litters gain more weight than rats growing up in normal-sized litters. Interestingly, these obese rats maintain this phenotype in adulthood. Conversely, rats raised in large litters show a delay in growth and a decrease in body weight. The aim of this work was to assess the hypothalamic control mechanisms of food intake regulated by perinatal feeding. Methods: Leptin levels were analysed using RIA. Leptin receptor mRNA levels were analysed using RT-PCR. Neuropeptide mRNA levels were analysed using in situ hybridisation. Results: Perinatally overfed neonatal male rats exhibited hyperleptinaemia and a decrease in hypothalamic mRNA levels of the long isoform of the leptin receptor (OB-Rb), explaining their leptin resistance. Moreover, this obese model showed an increase in the mRNA expression of cocaine-and amphetamine-regulated transcript, neuropeptide Y and agouti-related protein in the hypothalamic arcuate nucleus (ARC). In contrast, perinatally underfed neonatal male rats with hypoleptinaemia showed an increase in hypothalamic mRNA of the short isoforms of the leptin receptor. Furthermore, they exhibited an increase in expression of neuropeptide Y and agoutirelated protein in the ARC. Conclusions/interpretation: Rats overfed during early postnatal life show a leptinresistant state mediated by down-regulation of the hypothalamic OB-Rb. These data, together with the increased expression of neuropeptide Y and agouti-related protein in specific neurons in the ARC, might indicate the existence of regulated programming in this nucleus and may provide a new aetiopathogenic concept in susceptibility to obesity.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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A possible role of neuropeptide Y, agouti-related protein and leptin receptor isoforms in hypothalamic programming by perinatal feeding in the rat

et al. 2004

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Food and Fluid Intake

Moran

Sakai

2003

Handbook of Psychology

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The ingestive behaviors of eating and drinking play primary roles in the maintenance of fluid homeostasis and energy balance. Eating and drinking occur intermittently, they both renew and anticipate depletions, and their controls are complex and redundant. The physiological controls of food intake can be divided into systems that mediate: (1) signals related to metabolic state, especially the degree of adiposity stores, (2) affective signals related to taste and nutritional consequences that serve to reinforce aspects of ingestive behavior, and (3) signals that arise within an individual meal that produce satiety. Interactions among these systems permit the appropriate patterning of feeding that lead to the overall regulation of energy balance. The controls of fluid balance depend on interactions among three relatively well characterized systems related to: (1) neural and hormonal signals arising from the detection of plasma osmolality and extracellular fluid volume, which influence the initiation of bouts of fluid ingestion; (2) neural and hormonal signals related to myriad factors that lead to satiety and thus terminate bouts of drinking; and (3) brain sites that receive and integrate these signals and which elicit appropriate physiological and behavioral responses.

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The hypocretins: Excitatory neuromodulatory peptides for multiple homeostatic systems, including sleep and feeding

2000

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Leptin Regulation of Prepro-orexin and Orexin Receptor mRNA Levels in the Hypothalamus

Cited by 180 publications

References 20 publications

A possible role of neuropeptide Y, agouti-related protein and leptin receptor isoforms in hypothalamic programming by perinatal feeding in the rat

A possible role of neuropeptide Y, agouti-related protein and leptin receptor isoforms in hypothalamic programming by perinatal feeding in the rat

Food and Fluid Intake

The hypocretins: Excitatory neuromodulatory peptides for multiple homeostatic systems, including sleep and feeding

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