Leptosphaeria maculans, a fungal pathogen of Brassica napus, secretes a subtilisin-like serine protease

Wilson, Lewis; Howlett, Barbara J.

doi:10.1007/s10658-004-7088-7

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…This research, along with studies on particular genes or randomly mutated genes Idnurm and Howlett, 2003;Idnurm et al, 2003b;Meyer et al, 2004) has resulted in additional annotated genes published on international databases e.g. opsin, isocitrate lyase, cyanide hydratase, neutral trehalase, secreted proteins sp1 and sp2, G a subunit Idnurm and Howlett, 2002;Wilson et al, 2002;Idnurm et al, 2003b;Wilson and Howlett, 2005; M. Meyer, this study) (Figure 2).…”

Section: Chromosome Length Polymorphismsmentioning

confidence: 81%

Genetic Linkage Maps and Genomic Organization in Leptosphaeria maculans

Kühn¹,

Gout²,

Howlett

et al. 2006

Eur J Plant Pathol

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Leptosphaeria maculans is a haploid outcrossing ascomycete with a genome size of about 34 Megabases (Mb) which is predicted to have between 10,000 and 12,000 genes. The chromosomes of L. maculans are of a size range (0.7-3.5 Mb) and number (15-16) that can be readily resolved by pulsed field gel electrophoresis. Chromosome length polymorphisms are generated by meiosis giving rise to size differences as high as 57%, in the case of the ribosomal DNA-harbouring chromosome whose size varies between 1.8 and 4.2 Mb. Genetic maps are characterised by linkage groups comprising an accumulation of markers based on retrotransposon sequences. This, along with sequencing of pericentromeric regions and stretches of ORF-rich regions, suggest that the genome of L. maculans consists of a mosaic of GC-equilibrated coding regions with no or few transposons, and of stretches of highly degenerated and truncated retrotransposons, encompassing very few genes. Chromosome length polymorphisms are linked with the loss of dispensable regions. We suggest that the degree of length polymorphism for a particular chromosome correlates to the amount of dispensable retrotransposons, and that some gene-rich chromosomes may be less prone to undergo chromosome length polymorphisms than other chromosomes.

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Section: Chromosome Length Polymorphismsmentioning

confidence: 81%

Genetic Linkage Maps and Genomic Organization in Leptosphaeria maculans

Kühn¹,

Gout²,

Howlett

et al. 2006

Eur J Plant Pathol

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“…Sexton et al (2000) have cloned a gene encoding endo-PG, pg1, and two genes encoding cellulases, cel1 and cel2, in L. maculans. The sp1 and sp2 genes are also expressed in L. maculans during the infection of B. napus plants, and later the gene secretes a serine protease with protease activity (Wilson and Howlett 2005). The Lmpma 1 gene of L. maculans encodes a plasma membrane H+-ATPase isoform, which is essential for pathogenicity toward oilseed rape (Remy et al 2008).…”

Section: Pathogenmentioning

confidence: 99%

Diseases of Edible Oilseed Crops

Chattopadhyay¹,

Kolte²,

Waliyar³

2015

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AgriculturalSciences. He has more than 22 years of research experience in oilseed crop disease management and has significantly contributed in the areas of understanding the host-pathogen interactions, epi demiology, and integrated disease management in sunflower, safflower, sesame, peanut, and rape seed-mustard crops. He has published more than 70 peer-reviewed research papers and 4 books. Dr. Chattopadhyay has to his credit several academic awards and one patent. His significant research achievements include the development of Fusarium wilt-resistant genotypes in safflower, the iden tification of genotypes of Brassica juncea resistant to a mixture of isolates of Albugo candida, the development of a biocontrol agent formulation to reduce the use of fungicides in the management of major diseases of oilseeds (Brassica), and seed treatment (and foliar application) of aqueous bulb extract of Allium sativum for the management of major foliar diseases (Alternaria blight, white rust, powdery mildew, and Sclerotinia stem rot) of mustard. S.J. Kolte, professor of plant pathology (Retd.

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“…Thirteen of the proteins found were hypothetical proteins with unknown functions. Among the detected proteins the previously described secreted proteins 1 29 and 2 30 were also found. They occurred among the 18 proteins that were detected in all three protein fractions pF1-pF3 after IEF ( Figure 10A).…”

Section: Ms Analysis Of Protein Fractions After Ief With Elicitation mentioning

confidence: 82%

“…They are also highly expressed during infection in B. napus . While the function of sp1 is not clear, sp2 has been identified as a subtilisin‐like serine protease and proposed as having firstly a nutritional role by degrading proteins in the plant apoplast during the intercellular growth of L. maculans , and secondly during the later stages, when L. maculans kills plant cells, sp2 may contribute to the degradation of plant cell walls . However, to our knowledge no report about their elicitation activity exists.…”

Section: Discussionmentioning

confidence: 99%

Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus

Nováková

Kim

Šašek

et al. 2016

Biotechnology Progress

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The Dothideomycete Leptosphaeria maculans, a worldwide fungal pathogen of oilseed rape (Brassica napus), secretes a broad spectrum of molecules into the cultivation medium during growth in vitro. Here, candidate elicitor molecules, which induce resistance in B. napus to L. maculans, were identified in the cultivation medium. The elicitation activity was indicated by increased transcription of pathogenesis-related gene 1 (PR1) and enhanced resistance of B. napus plants to the invasion of L. maculans. The elicitation activity was significantly lowered when the cultivation medium was heated to 80°C. Active components were further characterized by specific cleavage with the proteolytic enzymes trypsin and proteinase K and with glycosidases α-amylase and β-glucanase. The elicitor activity was eliminated by proteolytic digestion while glycosidases had no effect. The filtered medium was fractionated by either ion-exchange chromatography or isoelectric focusing. Mass spectrometry analysis of the most active fractions obtained by both separation procedures revealed predominantly enzymes that can be involved in the degradation of plant cell wall polysaccharides. This is the first study searching for L. maculans-specific secreted elicitors with a potential to be used as defense-activating agents in the protection of B. napus against L. maculans in agriculture. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:918-928, 2016.

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Leptosphaeria maculans, a fungal pathogen of Brassica napus, secretes a subtilisin-like serine protease

Cited by 9 publications

References 24 publications

Genetic Linkage Maps and Genomic Organization in Leptosphaeria maculans

Genetic Linkage Maps and Genomic Organization in Leptosphaeria maculans

Diseases of Edible Oilseed Crops

Separation and identification of candidate protein elicitors from the cultivation medium of Leptosphaeria maculans inducing resistance in Brassica napus

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