Leptospira Detection in Cats in Spain by Serology and Molecular Techniques

Murillo, Andrea; Cuenca, Rafaela; Serrano, Emmanuel; Marga, Goris; Ahmed, Ahmed; Cervantes, S.; Caparrós, Cristina; Vieitez, Verónica; Ladina, Andrea; Pastor, Josep

doi:10.3390/ijerph17051600

Cited by 27 publications

(39 citation statements)

References 42 publications

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“…The diagnosis of leptospirosis is primarily serologic. Leptospires can be separated from the blood or urine, but people are rarely attempted to culture because the organisms require a unique medium and weeks to grow 76 . Also, the standard serologic test is limited to reference laboratories.…”

Section: Crispr/cas–based In Vitro Diagnosticsmentioning

confidence: 99%

CRISPR‐Cas system for biomedical diagnostic platforms

Wang

Cui

2020

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Clustered short palindrome repeats with regular intervals, abbreviated as CRISPR, and functions as a self‐defense system for prokaryotes, detecting particular pathogenic nucleic acid, interfering with the functions of exoteric DNA, and protecting them against foreign invaders. In recent years, CRISPR has attracted increasing interests in the in vitro diagnostic field because of its inherent allele specificity, which is one of the critical factors for the successful application of this technology in the development of high‐precision treatment and diagnosis. Herein, this review article aims to provide an overview of CRISPR‐CRISPR associated proteins (Cas) based biomedical diagnostics, including the biological mechanism, biomaterials, and applications. This paper first briefly introduces the development history and biological characteristics of the CRISPR‐Cas system, and then summarizes the application status and development trend of the CRISPR‐Cas system in the detection and identification of particular pathogens, specifically displaying a brilliant prospect in the most recent outbreak of novel coronavirus (formerly named 2019‐nCoV). Moreover, its potential diagnostic power in oncogene mutations and single nucleotide variations detecting are assembled. Finally, we discuss challenges and future prospects of CRISPR‐Cas system based diagnostic platforms in biomedicine, hoping to further inspire the development of biomedical diagnostics.

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Section: Crispr/cas–based In Vitro Diagnosticsmentioning

confidence: 99%

CRISPR‐Cas system for biomedical diagnostic platforms

Wang

Cui

2020

VIEW

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“…Thirty-two serum samples from domestic short-haired cats that had been collected during a previous study on Leptospira spp. prevalence in cats in Spain were used in the current study [10]. Cats were part of a free-roaming cat spay program and shelter's animals neutering program.…”

Section: Selection Of Casesmentioning

confidence: 99%

“…A wide variety of mammalian species may be susceptible to Leptospira spp. infection including domestic cats [2] in America [3][4][5], Asia [6][7], Australia [8] and Europe [9][10].…”

Section: Introductionmentioning

confidence: 99%

Acute phase proteins and total antioxidant capacity in free-roaming cats infected by pathogenic leptospires

Picco¹,

Cuenca²,

Serrano³

et al. 2020

Preprint

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Background: Leptospirosis is a neglected but widespread zoonotic disease throughout the world. The vast majority of mammals are hosts of Leptospira spp., including domestic cats, species in which no consensus has been reached on the clinical presentation or diagnosis of the disease. The study of acute-phase proteins (APPs) and biomarkers of oxidative status would contribute to knowledge about the course of the disease in cats. In this report, we evaluated four APPs: Serum amyloid A-SAA, Haptoglobin–Hp, albumin and Paraoxonase 1-PON1 and the antioxidant response through Total Antioxidant Capacity-TAC, in 13 free-roaming cats naturally infected by pathogenic leptospires and 19 leptospires-free cats, through a Principal Component Analysis (PCA). Results: The PCA retained two principal components (PC1 and PC2), explaining 60.1% of the observed variability of the inflammatory proteins and the antioxidant TAC. PC1 was mainly associated with an inflammatory process, whereas PC2 correlated with the antioxidant status. The most contributing variables in PC1 where albumin (27.46%), SAA (24.71%), Hp (21.56%) and PON1 (20.69%). Variables with significant contribution to the PC2 were the antibody titre against Leptospira spp. (48.41%) and TAC (35.04%). Overall, the PCA revealed differences in inflammatory and antioxidant biomarkers in cats naturally infected with leptospires compared to leptospires-free cats.Conclusions: Increases in Serum SAA, Hp, and decreases in serum albumin concentrations and PON1 activity indicate an acute phase response in infected, DNA positive cats. Moreover, we found an increase in TAC serum concentrations indicating an antioxidant response in this infection, which was proportional to the antibody titre and not to the presence of bacterial DNA.

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“…A wide variety of mammalian species may be susceptible to Leptospira spp. infection including domestic cats [2] in America [3][4][5], Asia [6][7], Australia [8] and Europe [9][10].…”

Section: Introductionmentioning

confidence: 99%

Acute Phase Proteins and Total Antioxidant Capacity in Free-Roaming Cats Infected by Pathogenic Leptospires

Picco

Cuenca

Serrano

et al. 2020

Preprint

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Background: Leptospirosis is a neglected but widespread zoonotic disease throughout the world. The vast majority of mammals are hosts of Leptospira spp., including domestic cats. Though clinical signs of active leptospirosis are generally mild in cats, chronic infections might result in renal injury. However, there is no consensus either on the clinical presentation nor on the disease diagnosis in cats. The study of acute-phase proteins (APPs) and biomarkers of oxidative status would contribute to knowledge about the development ofthe disease in cats. In this report, we evaluated four APPs: Serum amyloid A-SAA, Haptoglobin–Hp, albumin and Paraoxonase 1-PON1 and the antioxidant response through Total Antioxidant Capacity-TAC, in 13 free-roaming cats naturally infected by pathogenic leptospires and 19 leptospires-free cats, through a Principal Component Analysis (PCA). Results: DNA positive cats showed higher serum SAA and Hp concentrations than seropositive cats and their leptospires-free counterparts. DNA positive individuals, however, had lower serum PON1 activity and albumin concentrations than seropositive and leptospires-free cats. On the other hand, the increase in TAC serum concentrations was positively associated with anti-leptospiral antibodies titre. The PCA retained two principal components (PC1 and PC2), explaining 60.1% of the observed variability of the inflammatory proteins and the antioxidant TAC. PC1 was mainly associated with an inflammatory process, whereas PC2 correlated with the antioxidant status. The most contributing variables in PC1 where albumin (27.46%), SAA (24.71%), Hp (21.56%) and PON1 (20.69%). Variables with significant contribution to the PC2 were the antibody titre against Leptospira spp. (48.41%) and TAC (35.04%). Overall the PCA revealed differences in inflammatory and antioxidant biomarkers in cats naturally infected with leptospires compared to leptospires-free cats.Conclusions: Increases in Serum SAA, Hp, and decreases in serum albumin concentrations and PON1 activity indicate an acute phase response in infected, DNA positive cats. Moreover, we found an increase in TAC serum concentrations indicating an antioxidant response in this infection, which is proportional to the antibody titre and not to the presence of bacterial DNA.

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Leptospira Detection in Cats in Spain by Serology and Molecular Techniques

Cited by 27 publications

References 42 publications

CRISPR‐Cas system for biomedical diagnostic platforms

CRISPR‐Cas system for biomedical diagnostic platforms

Acute phase proteins and total antioxidant capacity in free-roaming cats infected by pathogenic leptospires

Acute Phase Proteins and Total Antioxidant Capacity in Free-Roaming Cats Infected by Pathogenic Leptospires

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