The marine environment may change the force on the fluid and inevitably influence bubble behavior and the two-phase flow in the reactor core, which are vital to the safety margin of a nuclear reactor. To explore the effect of the marine motion on the flow and heat transfer features of subcooled flow boiling in the reactor core, the volume of fluid (VOF) method is employed to reveal the interaction between the interface structure and two-phase flow in the subchannel under rolling motion. The variations of several physical parameters are obtained, including the transverse flow, the vapor volume fraction, the vapor adhesion ratio, and the phase distribution of boiling two-phase flow with time. Sensitivity analyses of the amplitude and the period of the rolling motion were performed to demonstrate the mechanisms of the influence of the rolling motion. We found that the transverse flow in the subchannel was mainly affected by the Euler force under the rolling motion. In contrast to the two-phase flow in the static state, the vapor volume fraction and vapor adhesion ratio show different characteristics under rolling motion. Additionally, the onset of significant void (OSV) point changes periodically under rolling motion.