Les anomalies congénitales de glycosylation des N-glycosylprotéines

Lavieu, Grégory; Moore, Stuart; Seta, Nathalie

doi:10.1051/medsci/2004203331

Cited by 7 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the highest signal intensities in both spine and test ASMs of A. lixula were without exception obtained with lectins that preferably bind to motifs that contain N-acetyl-glucosamine (GlcNAc), such as STL, LEL, DSL and WGA. This suggests the presence of N-glycosylation [58,59], a finding that is in agreement with previous deglycosylation studies using endoglycosidase F or N-glycanase, which demonstrated the presence of N-glycosidic bonds in the organic matrix extracted from S. purpuratus embryos [18,23].…”

Section: Discussionsupporting

confidence: 92%

Spine and test skeletal matrices of the Mediterranean sea urchin Arbacia lixula – a comparative characterization of their sugar signature

et al. 2015

View full text Add to dashboard Cite

Calcified structures of sea urchins are biocomposite materials that comprise a minor fraction of organic macromolecules, such as proteins, glycoproteins and polysaccharides. These macromolecules are thought to collectively regulate mineral deposition during the process of calcification. When occluded, they modify the properties of the mineral. In the present study, the organic matrices (both soluble and insoluble in acetic acid) of spines and tests from the Mediterranean black sea urchin Arbacia lixula were extracted and characterized, in order to determine whether they exhibit similar biochemical signatures. Bulk characterizations were performed by mono-dimensional SDS/PAGE, FT-IR spectroscopy, and an in vitro crystallization assay. We concentrated our efforts on characterization of the sugar moieties. To this end, we determined the monosaccharide content of the soluble and insoluble organic matrices of A. lixula spines and tests by HPAE-PAD, together with their respective lectin-binding profiles via enzyme-linked lectin assay. Finally, we performed in situ localization of N-acetyl glucosamine-containing saccharides on spines and tests using gold-conjugated wheatgerm agglutinin. Our data show that the test and spine matrices exhibit different biochemical signatures with regard to their saccharidic fraction, suggesting that future studies should analyse the regulation of mineral deposition by the matrix in these two mineralized structures in detail. This study re-emphasizes the importance of non-protein moieties, i.e. sugars, in calcium carbonate systems, and highlights the need to clearly identify their function in the biomineralization process.

show abstract