Les cancers de la cavité buccale : affection à prédominance féminine à Ouagadougou

Bambara, Augustin Tozoula; Millogo, Mathieu; Konsem, Tarcissus; Bambara, Hierrhum Aboubacar; Ouédraogo, Dieu-Donné; Traoré, Si Simon

doi:10.1051/mbcb/2015011

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…The sex ratio in this study was 1.4 in favor of men. Some authors have described oral cavity cancers as a predominantly female disease in Ouagadougou, Burkina Faso [10].…”

Section: Hpv Genotypes Co-infection Number Of Casesmentioning

confidence: 99%

Detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

Kandé,

Ilboudo,

Ouermi

et al. 2023

JBM

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Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. These viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV ENT infections can lead to benign or malignant tumors in which we could find both LR-HPV and HR-HPV genotypes. The objective of this study was to investigate the genotypes of HR-HPV and LR-HPV in archived tissue samples derived from both benign and malignant tumors of the ear, nose, and throat (ENT) in Ouagadougou, Burkina Faso. One hundred and twenty formalin-fixed, paraffin-embedded archived tissues of the ENT sphere from 26 benign tumors and 94 malignant tumors were included. The tissues were first deparaffinized with xylem. The extracted DNA was used to test for high-risk and low-risk HPV by Real-Time Multiplex PCR. HPV DNA was found in 57.7% (15/26) of benign tumors and 43.61% (41/94) of malignant tumors. The prevalence of HPV infection was 46.67% (56/120) in all tumors combined. The most common HPV genotypes found were HPV 11 (34.28%), HPV 6 (30%), HPV56 (14.28%) and HPV 33 (8.57%). There were 21.43% (12/56) cases of genotypes co-infections with 10 cases of double infection and 2 cases of triple infection. Both low-risk and high-risk HPV are found in ENT tumors with relatively high HPV prevalence.

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“…The sex ratio in this study was 1.4 in favor of men. Some authors have described oral cavity cancers as a predominantly female disease in Ouagadougou, Burkina Faso [10].…”

Section: Hpv Genotypes Co-infection Number Of Casesmentioning

confidence: 99%

Detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

Kandé,

Ilboudo,

Ouermi

et al. 2023

JBM

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Genetic Alterations of C-MYC Proto-Oncogene and Their Involvement in the Occurrence and Progression of Oral Cavity Cancers in Senegal

Ba,

Mbaye,

Diatta

et al. 2023

J. Mol. Clin. Med.

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Background: Oral cancer is the 17th most common cancer worldwide, with a mortality rate of 1.8%. Their incidence varies considerably, with a clear prevalence in South Asian countries. In Africa, the mortality rate for cancers of the oral cavity is 1.3%. Senegal is a perfect illustration a perfect illustration of the seriousness and scale of this disease, with 177 new cases recorded in 2020, for a mortality rate of 1.4%. To add to the knowledge of the molecular mechanisms involved in the carcinogenesis of these pathologies in Senegal, mutations in the C-MYC proto-oncogene were examined in 22 patients with oral cavity cancers and compared with samples from 32 control individuals. Methods: Cancerous tissue (CT) and adjacent normal tissue (ANT) were sampled from diseased individuals, whereas whole blood was obtained from control individuals (C). A total of 67 samples were collected: 32 from controls, 22 from CTs, and 13 from ANTs of diseased individuals. Total DNA was extracted and polymerase chain reaction (PCR) amplification of exon 2 of the C-MYC gene was performed, followed by Sanger sequencing. Mutation analysis was performed using Mutation Surveyor Software v5.0.1. The effect of each non-synonymous mutation on the function of the encoded protein was determined using the POLYPHEN-2, PANTHER-PSEP, and PROVEAN algorithms. The probability of non-synonymous mutations causing diseases was predicted using Prediction of human Deleterious Single Nucleotide Polymorphism (PhD-SNP) and Predicting disease associated variations using GO terms (SNP&GO). The impact of non-synonymous variations on the stability of the encoded protein was determined using I-Mutant2 and In-silico analysis of Protein Stability (INPS). Results: Of the study participants, 63% were females. The mean age of patients was 46.43 ± 13 years, with extremes of 14 and 83 years and the age range of 40–70 years as the most representative age group. Only 5% of patients were alcohol drinkers and 15% were smokers. Most patients (80%) had stage III or IV tumors with lymph node invasion. A low polymorphism rate in exon 2 of the C-MYC proto-oncogene was identified, with one synonymous substitution (Q48Q) found in a diseased individual (CT and ANT). The non-synonymous substitutions (D31N, D31E, V33G, Y36N, and Y36D) found in the controls were predicted to be damaging and pathogenic, and might decrease the stability of the encoded protein. Conclusions: Our results indicate that the C-MYC protooncogene is not involved in the occurrence and progression of oral cavity cancers in Senegalese patients. However, the mutations found in controls could provide new markers for the early clinical diagnosis of oral cancer.

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Les cancers de la cavité buccale : affection à prédominance féminine à Ouagadougou

Cited by 2 publications

References 12 publications

Detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

Detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

Genetic Alterations of C-MYC Proto-Oncogene and Their Involvement in the Occurrence and Progression of Oral Cavity Cancers in Senegal

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