Less common applications of monoliths: Preconcentration and solid-phase extraction

Švec, František

doi:10.1016/j.jchromb.2006.03.055

Cited by 196 publications

(125 citation statements)

References 80 publications

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“…A buffer of sodium dihydrogen phosphate (0.02 M), 20% (v/v) acetonitrile was prepared by adjusting the solution pH with sodium hydroxide or phosphoric acid to the required pH. 5-phosphorylated oligodeoxythymidylic acids fragments [d(pT) [12][13][14][15][16][17][18] ] sample was purchased from Sigma (Vienna, Austria). Low-range mutation standard [STS marker from the Y-chromosome (209 bp)] was purchased from Transgenomics (Berlin, Germany).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…5,6 Monoliths have been prepared from different materials such as inorganic silica by sol-gel [7][8][9] or organic polymer such as metal-catalyzed ring opening metathesis polymerization (ROMP), 10 or free-radical polymerization based on styrene and acrylate chemistry. [11][12][13] The ease to make and derivatize, made these monoliths a good choice for present and future applications in bioseparation.…”

Section: Introductionmentioning

confidence: 99%

“…These monoliths were surface modified into strong anion-exchangers with hydrochloric acid (10%) and triethylamine, successively. The strong anion-exchanger established good separation of 5-phosphorylated oligodeoxythymidylic acids fragments [d(pT) [12][13][14][15][16][17][18] ]. Moreover, heteroduplex and homoduplex fragments of a low-range mutation standard [of STS marker from the Y-chromosome (209 bp)] were separated at ambient and elevated temperatures using sodium phosphate buffer and a gradient former of sodium chloride, in anion-exchange high-performance liquid chromatography (AE-HPLC).…”

mentioning

confidence: 99%

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Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Lubbad

2015

ACSi

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Monoliths of poly (glycidylmethacrylate-co-divinylbenzene) were prepared in the confines of presilanized borosilicate glass columns (100 × 3 mm I.D.). These monoliths were surface modified into strong anion-exchangers with hydrochloric acid (10%) and triethylamine, successively. The strong anion-exchanger established good separation of 5-phosphorylated oligodeoxythymidylic acids fragments [d(pT) [12][13][14][15][16][17][18] ]. Moreover, heteroduplex and homoduplex fragments of a low-range mutation standard [of STS marker from the Y-chromosome (209 bp)] were separated at ambient and elevated temperatures using sodium phosphate buffer and a gradient former of sodium chloride, in anion-exchange high-performance liquid chromatography (AE-HPLC). This is a step forward for mutation detection as temperature-independent method, which is not the case in denatured ion-paired reversed-phase chromatography (D-IP-RP-HPLC), where mutation detection is temperature critical and might be bypassed if temperature changes slightly. Finally, reproducibility check from run-to-run and monolith-to-monolith showed a relative standard deviation (RSD) of less than 2%.

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Section: Chemicals and Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Lubbad

2015

ACSi

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“…Modern trends in sample preparation for food applications include the use of on-line solid phase extraction (SPE) methods or the use of more SPE-based selective approaches, such as molecularly-imprinted polymers (MIPs) [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances and Uses of Monolithic Columns for the Analysis of Residues and Contaminants in Food

et al. 2015

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Abstract:Monolithic columns are gaining interest as excellent substitutes to conventional particle-packed columns. These columns show higher permeability and lower flow resistance than conventional liquid chromatography columns, providing high-throughput performance, resolution and separation in short run times. Monoliths possess also great potential for the clean-up and preparation of complex mixtures. In situ polymerization inside appropriate supports allows the development of several microextraction formats, such as in-tube solid-phase and pipette tip-based extractions. These techniques using porous monoliths offer several advantages, including miniaturization and on-line coupling with analytical instruments. Additionally, monoliths are ideal support media for imprinting template-specific sites, resulting in the so-called molecularly-imprinted monoliths, with ultra-high selectivity. In this review, time-saving LC columns and preparative applications applied to the analysis of residues and contaminants in food in 2010-2014 are described, focusing on recent improvements in design and with emphasis in automated on-line systems and innovative materials and formats.

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“…Silica monoliths have been applied to capillary-HPLC-MS [24]. Organic monoliths have been applied to solid-phase extraction, preconcentration, and on a large plant scale for purification [25] [26] and both silica and organic have been applied to capillary electrochromatography, [27]. Monoliths do; however, suffer from some drawbacks including poor tolerance to alkaline mobile phases, high solvent consumption and in some cases need heating or cooling.…”

mentioning

confidence: 99%

Separation of oxidatively damaged DNA nucleobases and nucleosides on packed and monolith C18 columns by HPLC-UV-EC

Kelly

White

Smyth

2008

Journal of Chromatography B

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This study involves the incorporation of a commercially available Phenomenex Onyx C18 monolith column into the separation and detection of oxidative DNA damage. It includes thorough investigation of monolith performance and a comparison of the performance of monolith columns with a commercially available packed Restek reverse phase Ultra C18 column for the separation of DNA bases and nucleosides. The performance of the monolith was examined using efficiency, resolution, plate height, asymmetry and retention times, and in each case showed improved or at least comparable results in the separation of a mix of DNA bases and nucleosides. A 90% reduction, from just under 40 min. to just under 4 min., was obtained in the elution time of this separation. To the best of our knowledge, this is the first report of a fast monolith column separation successfully coupled to both a UV-vis and EC detector, which is especially useful for analysis of oxidative DNA damage. The determination of 8-oxoG and 8-OH-dG, oxidation products of guanine and 2'-deoxyguanosine, respectively, may be compromised by their ease of oxidation and therefore the fast separation, selective and sensitive detection, with no artifactual oxidation, detailed in this report, is ideal.

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Less common applications of monoliths: Preconcentration and solid-phase extraction

Cited by 196 publications

References 80 publications

Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Recent Advances and Uses of Monolithic Columns for the Analysis of Residues and Contaminants in Food

Separation of oxidatively damaged DNA nucleobases and nucleosides on packed and monolith C18 columns by HPLC-UV-EC

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