Let‐7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin

Yan, Shaofeng; Han, Xiao; Xue, Hao; Zhang, Ping; Guo, Xing; Li, Tong; Guo, Xiaofan; Yuan, Gang; Deng, Lin; Li, Gang

doi:10.1002/jcb.25128

Cited by 31 publications

(37 citation statements)

References 43 publications

(68 reference statements)

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“…MiRNAs often suppressed expression of multiple proteins by inhibiting translation or targeting mRNAs at 3′UTR for degradation [19]. According the previous reports, current bioinformatics tools for miRNA-target analysis were widely used in studying association between miRNAs and diseases, including cancer, inflammation, neurodegenerative diseases and epilepsy [12, 13, 15, 20]. These studies indicated these miRNA target prediction tools were effective and accuracy for miRNA target prediction.…”

Section: Discussionmentioning

confidence: 99%

“…The primers of miRNAs were purchased from RIBO (Guangzhou, China). Real-time PCR for miRNAs was performed according to previous reports in triplicate for each sample [20]. The relative amount of each miRNA was normalized against cel-miR-39-3p by the 2 −ΔΔCt method.…”

Section: Methodsmentioning

confidence: 99%

“…The target genes of differentially exosomal miRNAs were predicted by TargetScan (http://www.targetscan.org/) microRNA (http:/www.microrna.org/microrna/home.do) and miRBase (http://www.mirbase.org) [20]. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analyses were performed by a DAVID online analysis tool (http://david.abcc.ncifcrf.gov/) [34, 35].…”

Section: Methodsmentioning

confidence: 99%

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Altered microRNA profiles in plasma exosomes from mesial temporal lobe epilepsy with hippocampal sclerosis

et al. 2016

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Mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE-HS) is the most common type of focal epilepsy. The present study aimed to explore the expression and functions of exosomal microRNAs in mTLE-HS. A total of 50 microRNAs were found to be differentially expressed in mTLE-HS compared with healthy controls. Among them, 2 were increased and 48 were decreased. The 6 significant differentially expressed candidate microRNAs (miR-3613-5p, miR-4668-5p, miR-8071, miR-197-5p, miR-4322, and miR-6781-5p ) in exosome were validated. The bioinformatics analysis showed that the potential target genes of these microRNAs were involved in biological processes, molecular functions, and cellular components. Similarly, these microRNAs also affected axon guidance, pathways in cancer, regulation of the actin cytoskeleton, focal adhesion, the calcium signaling pathway, the MAPK signaling pathway, and the PI3K-Akt signaling pathway. Among 6 candidate microRNAs, miR-8071 had the best diagnostic value for mTLE-HS with 83.33% sensitivity and 96.67% specificity, and was associated with seizure severity. This study indicated that exosomal microRNAs, may be regulators for the seizure development in mTLE-HS, and can be used as potential therapeutic targets and biomarker for diagnosis in mTLE-HS.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Altered microRNA profiles in plasma exosomes from mesial temporal lobe epilepsy with hippocampal sclerosis

et al. 2016

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“…[1][2][3][4][5] Although many mechanisms have been elucidated in the pathogenesis of hypertension, such as endothelial dysfunction, 6 sympathetic nervous system overdrive, 7 inflammatory reactions, 8 oxidative stress, 9 gene modifications and impaired angiogenesis, 10 at the molecular level, many aspects of hypertension development are still unknown. 11 MicroRNAs (miRs) are small non-coding RNAs that are key posttranscriptional regulators of gene expression in all eukaryotic cells 12 and have been found to modulate the function of endothelial cells (ECs), 13 smooth muscle cells 14 and macrophages 15 by controlling the expression of chemokines, thereby affecting different stages of atherosclerosis progression. 16 Specifically, miR-92a has been reported to have a vital role in the development of atherosclerosis by modulating ECs and smooth muscle cells.…”

Section: Introductionmentioning

confidence: 99%

Circulating miR-92a expression level in patients with essential hypertension: a potential marker of atherosclerosis

Huang

Tang²,

Ji-yan

et al. 2016

J Hum Hypertens

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MicroRNAs (miRs) are key posttranscriptional regulators of gene expression in all eukaryotic cells and have a vital role in the evolution of hypertension and cardiovascular remodelling and, therefore, have emerged as potential biomarkers for cardiovascular disease. We assessed 240 participants, including 60 healthy volunteers with normal carotid intima-media thickness (nCIMT), 60 healthy volunteers with increased CIMT (iCIMT), 60 hypertensive patients with nCIMT and 60 hypertensive patients with iCIMT. All patients underwent measurements of CIMT, carotid-femoral pulse wave velocity (cfPWV) and ambulatory blood pressure (BP) monitoring. Plasma miR-92a expression was quantified by real-time reverse transcription PCR. Correlations between miR-92a expression and BP parameters, CIMT and cfPWV were assessed using the Spearman correlation coefficient. We observed the lowest miR-92a expression (24.59±1.30 vs 27.76±2.13 vs 29.29±1.89 vs 33.76±2.08; P<0.001) in healthy controls with nCIMT, followed by healthy controls with iCIMT, then hypertensive patients with nCIMT and the highest expression in hypertensive patients with iCIMT. Additionally, MiR-92a levels showed a significant positive correlation with 24-h mean systolic BP (r=0.807, P<0.001), 24-h mean diastolic BP (r=0.649, P<0.001), 24-h mean pulse pressure (PP) (r=0.697, P<0.001), 24-h daytime PP (r=0.654, P<0.001), 24-h nighttime PP (r=0.573, P<0.001), CIMT (r=0.571, P<0.001) and cfPWV (r=0.601, P<0.001). Our data present significant evidence that circulating miR-92a represents a potential noninvasive atherosclerosis marker in essential hypertensive patients.

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“…Furthermore, a study has indicated the suppressive influence of let‐7a over‐expression on glioma cell malignancy . Additionally, let‐7f targets periostin has been reported to repress glioma cell invasion, migration, and proliferation . The human miR cluster MC‐let‐7a‐1 ~ let‐7d, including let‐7d, let‐7f‐1, and let‐7a‐1, has been shown to exhibit low levels in hepatocellular carcinoma (HCC) cells .…”

Section: Introductionmentioning

confidence: 99%

microRNA cluster MC‐let‐7a‐1~let‐7d promotes autophagy and apoptosis of glioma cells by down‐regulating STAT3

et al. 2019

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Background Accumulating evidence has highlighted the correlation between microRNAs (miRNAs) and the progression of glioma. However, the role of miR cluster MC‐let‐7a‐1 ~ let‐7d in glioma remains elusive. Thus, the current study aimed to investigate the effect of miR cluster MC‐let‐7a‐1 ~ let‐7d on glioma progression. Methods and Results Microarray data analysis provided data indicating the involvement of miR cluster MC‐let‐7a‐1 ~ let‐7d in glioma via STAT3. The expression of let‐7a‐1, let‐7d, let‐7f‐1, and miR cluster MC‐let‐7a‐1 ~ let‐7d was diminished in the glioma tissues and the cell lines. Additionally, our results revealed that STAT3 was a target gene of let‐7d, let‐7a‐1, and let‐7f‐1, which was further verified by the dual‐luciferase reporter gene assay. Moreover, STAT3 expression was negatively mediated by let‐7a‐1, let‐7d, and let‐7f‐1. Up‐regulated miR cluster MC‐let‐7a‐1 ~ let‐7d or silenced STAT3 suppressed cell proliferation but accelerated cell apoptosis and autophagy. Moreover, restrained tumor growth was identified in the nude mice treated with miR cluster MC‐let‐7a‐1 ~ let‐7d mimics or STAT3 siRNA. Conclusion Taken together, the miR cluster MC‐let‐7a‐1 ~ let‐7d promotes glioma cell autophagy and apoptosis by repressing STAT3. The current study highlights the potential of the miR cluster MC‐let‐7a‐1 ~ let‐7d as biomarkers and promising treatment strategies for glioma.

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Let‐7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin

Cited by 31 publications

References 43 publications

Altered microRNA profiles in plasma exosomes from mesial temporal lobe epilepsy with hippocampal sclerosis

Altered microRNA profiles in plasma exosomes from mesial temporal lobe epilepsy with hippocampal sclerosis

Circulating miR-92a expression level in patients with essential hypertension: a potential marker of atherosclerosis

microRNA cluster MC‐let‐7a‐1~let‐7d promotes autophagy and apoptosis of glioma cells by down‐regulating STAT3

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