Lethal photosensitization in microbiological treatment of ligature-induced peri-implantitis: a preliminary study in dogs

Shibli, Jamil Awad; Martins, Maristela Santini; Theodoro, Letícia Helena; Lotufo, Roberto Fraga Moreira; Garcia, Valdir Gouveia; Marcantônio, Élcio

doi:10.2334/josnusd.45.17

Cited by 68 publications

(61 citation statements)

References 31 publications

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“…On the other hand, based on the results of the present study only P. gingivalis counts in the control group and A. actinomycetemcomitans, P. gingivalis and T. forsythia counts in the test group decreased significantly; in this context, the bacterial species were similar to those in the study carried out by Bassetti et al 40 In a study, the effect of PDT with toluidine blue on periodontopathogens and biofilm on tooth surfaces was evaluated and it was reported that the use of diode laser beams at a wavelength of 830 nm in association with the use of toluidine blue within periodontal pockets was effective in destroying bacteria and it might be a reliable alternative for antimicrobial treatment in periodontitis. 41 Therefore, the results of the present study are consistent with studies carried on by Shibli et al, 38 Kocher and Mecisel 20 and the study mentioned above. PDT with a combination of laser and EmunDo dye resulted in destruction of bacteria and in a decrease in bacterial counts, indicating that the type of the dye does not influence the antibacterial effect and the presence of a different photosensitizing dye results in similar antibacterial effects.…”

Section: Effectiveness Of Photodynamic Therapy In Treatment Of Peri-isupporting

confidence: 83%

“…The majority of reports indicate that PDT only results in decreasing the inflammation severity and there is insufficient evidence in relation to the effect of PDT on periodontitis. [35][36][37] Shibli et al 38 showed the effect of PDT on the pathogens within periodontal pockets. Meisel and Kocher 20 showed that the use of photosensitizing dyes and their activation with laser beams might result in the destruction of pathogens responsible for periodontal diseases and peri-implantitis.…”

Section: Effectiveness Of Photodynamic Therapy In Treatment Of Peri-imentioning

confidence: 99%

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Evaluation of Effectiveness of Photodynamic Therapy With Low-level Diode Laser in Nonsurgical Treatment of Peri-implantitis

et al. 2017

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Introduction: Side effects related to antibiotic therapy for peri-implantitis are rare in laser therapy (LT); therefore, the aim of this study was to evaluate the effectiveness of LT and photodynamic therapy (PDT) on patients with primary peri-implantitis. Methods: In this randomized clinical trial, 40 implants presenting primary peri-implantitis in 20 patients with a mean age of 52.6 years old were included using the simple sampling technique. Periodontal treatment comprising scaling and root planing (SRP) was accomplished for the whole mouth while mechanical debridement with titanium curettes and air polishing with sodium bicarbonate powder was accomplished around the implants. The implants were randomly divided into two groups and treated with LT (control) and PDT (test). The clinical indices were measured at baseline, 6 weeks and 3 months after treatment. Real-time polymerase chain reaction (PCR) was used for analysis of microbial samples at baseline and 3-month follow-up. Data were analyzed with SPSS 20, using repeated-measures analysis of variance (ANOVA) and Friedman's and MannWhitney tests (α = 0.05). Results: Both groups showed statistically significant improvements in terms of bleeding on probing (P < 0.001), probing pocket depth (PPD) (P = 0.006) and modified plaque index (P < 0.001), with no significant differences between the 2 groups (P > 0.05). The number of Aggregatibacter actinomycetemcomitans (P = 0.022), Tannerella forsythia (P = 0.038) and Porphyromonas gingivalis (P = 0.05) in the test group and Porphyromonas gingivalis (P = 0.015) in the control group significantly decreased. Conclusion:The results suggested that LT and PDT have significant short-term benefits in the treatment of primary peri-implantitis.

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Section: Effectiveness Of Photodynamic Therapy In Treatment Of Peri-isupporting

confidence: 83%

Section: Effectiveness Of Photodynamic Therapy In Treatment Of Peri-imentioning

confidence: 99%

Evaluation of Effectiveness of Photodynamic Therapy With Low-level Diode Laser in Nonsurgical Treatment of Peri-implantitis

et al. 2017

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“…Other authors have shown that this form of treatment is effective against various infections, including by bacteria, fungi, and viruses [31][32][33][34]. The present investigation has extended these studies in showing that the combination of methylene blue and a diode laser is also effective for bacterial reduction.…”

Section: Discussionsupporting

confidence: 70%

Antimicrobial effect of photodynamic therapy using methylene blue and red color diode laser on biofilm attached to sandblasted and acid-etched surface of titanium

et al. 2017

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Purpose The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) on biofilm attached to sandblasted and acid-etched (SLA) surfaces. Methods The bacterial strains used were Aggregatibacter actinomycetemcomitans and Streptococcus sanguinis. SLA disks were subdivided into five groups including one control group and four test groups (laser-alone, methylene blue (MB)-alone, PDT60, and PDT120) for PDT examination for each surface. The laser-alone group was only irradiated with a diode laser for 60 s. The MB-alone group was put into 1 ml of 100 μg/ml of methylene blue for 60 s. PDT60 and PDT120 groups were put into 1 ml of 100 μg/ml methylene blue and then irradiated for 60 and 120 s, respectively. After treatment, the survival rate of bacteria was determined by counting the colony-forming units (CFUs) after incubation. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were used to observe the microorganisms on the disk surfaces. Results Significant differences were found between the control group, LED-alone group, MB-alone group, and PDT60 and PDT120 groups (P < 0.05). The PDT60 and PDT120 groups showed a decreasing tendency of live bacteria. In the SEM images, the control group, LED-alone group, and MBalone group showed intact bacterial cell walls. However, the bacterial cell walls were changed after PDT. On CLSM images, the PDT60 and PDT120 groups showed a high proportion of dead bacteria with red color. Conclusions These findings suggest that combination of methylene blue and a red color LED (Periowave®) was effective in reducing the viability of biofilm attached to SLA titanium surface in vitro.

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“…However, different types of wavelengths can help the clinicians to kill all the bacteria living of the implant surface [86,87]. Results of the recent studies show that antibacterial PDT is a promising method for the treatment of perimplantitis [88,89].…”

Section: Dentistrymentioning

confidence: 99%

Biomedical Optics and Lasers

Sener¹

2012

A Roadmap of Biomedical Engineers and Milestones

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Lethal photosensitization in microbiological treatment of ligature-induced peri-implantitis: a preliminary study in dogs

Cited by 68 publications

References 31 publications

Evaluation of Effectiveness of Photodynamic Therapy With Low-level Diode Laser in Nonsurgical Treatment of Peri-implantitis

Evaluation of Effectiveness of Photodynamic Therapy With Low-level Diode Laser in Nonsurgical Treatment of Peri-implantitis

Antimicrobial effect of photodynamic therapy using methylene blue and red color diode laser on biofilm attached to sandblasted and acid-etched surface of titanium

Biomedical Optics and Lasers

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