Lethal toxicity and immunogenicity of salmolysin, Aeromonas salmonicida hemolysin to white-spotted char, Salvelinus leucomaenis.

Extracellular glycerophospholipid:cholesterol acyltransferase from Aeromonas salmonicida: activation by serine protease

“…Similar testing of free GCAT has not been performed. These relatively high lethal doses are not readily eomparable with those previously published for GCAT-LPS (Lee & Ellis 1989) or salmolysin (Nomura et al 1988;Kawahara & Nomura 1989;Kawahara, Oshima & Nomura 1990) due to the different methods of preparation and administration. In line with this, the high molecular weight salmolysin purified by Nomura et al (1988) showed a high pereentage of carbohydrates, but contained as much as 38% protein and was clearly different from our GCAT-LPS preparation.…”

“…In addition, LPS-frce GCAT purified by Huntly, Coleman & Munro (1992) had lethal effeets on Atlantic salmon, Salnw salar L. A 56-kDa protein, haemolytie to salmonid erythroeytcs, was shown to induce furuncle-like lesions when mixed with the 70-kDa serine protease from the same bacterium and injected intra-muscularjy into Atlantic salmon (Fyfe,Coleman & Munro (987,198N). A glyeoprotein of 20(] kDa or larger was found to be haemolytie to salmonid erythrocytes and lethal to white-spotted ehar, Salvelinus leucomaenis, (Nomura, Fujino, Yamakawa & Kawahara 1988;Kawahara & Nomura 1989). In addition to haemolysins, A. salmonicida also secretes a 7n-kDa serine protease (Fyfc, Finley, Coleman & Munro 1986;Lee & Ellis 1989) and metalloproteases (Sheeran & Smith 1981;Price, Stevens, Duncan & Snodgrass 1989).…”

Toxicity and immunogenicity of Aeromonas salmonicida extracellular products to salmonids

“…Nomura et al (1988) demonstrated that fingerling rainbow trout weighing about 6 3g died 4-7 days following intramuscular injection of purified salmolysin and the LD5(| value was estimated as approximately 58^g/kg. Kawahara & Nomura (1989) reported the LD50 of salmolysin for juvenile white-spotted char weighing about 8*3 g was estimated at 196 and >556//g/kg body weight for intramuscular and intraperitoneal injections, respectively. Fyfe et aL (1988) showed that ECP and protease-haemolysin mixtures had similar pathologic effects in Atlantic salmon following intramuscular injection.…”

“…Nomura et al (1988) demonstrated purified salmolysin possessed antigenicity to rabbits. Kawahara & Nomura (1989) showed salmolysin was detoxified by either heating at lWC for 10min, or incubation at 37°C for a week with 0*4% formalin. Immunogenicity of each detoxified toxin in white-spotted char was detected.…”

Injection vaccination of white-spotted char, Salvelinus leucomaenis, against furunculosis with Aeromonas salmonicida salmolysin.

“…Kawahara and Nomura (1989) reported the the humoral immune response of white-spotted char against salmolysin detoxified by heating or formalin treatment. After intramuscular injec tion of detoxified salmolysins, serum antibody t iter was raised and remarked 1: 16 to 1: 64 after three to five weeks.…”