Lethality enhancement of pressure-assisted thermal processing against Bacillus amyloliquefaciens spores in low-acid media using antimicrobial compounds

Daryaei, Hossein; Balasubramaniam, V.M.; Yousef, Ahmed E.; Legan, J.D.; Tay, Abdullatif

doi:10.1016/j.foodcont.2015.05.029

Cited by 16 publications

(9 citation statements)

References 50 publications

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“…It is also noteworthy that the utilization of the HEPES buffer is common in the high-pressure processing literature, since the medium could maintain a buffered environment under the elevated hydrostatic pressure [ 34 ]. Thus, the reductions obtained in the current study could be attributed to the effects of elevated hydrostatic pressure, heat, and the antimicrobials rather than any intrinsic factor of the medium [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…To assure further recovery of injured cells, 0.6% of yeast extract was added to Tryptic Soy Agar (TSA, Difco, Becton Dickinson, Franklin Lakes, NJ, USA), the medium used for enumeration of the pathogen. The use of tryptic soy agar with yeast extract (TSA + YE) additionally enhances the recovery of pressure-, heat- and antimicrobial-injured microbial cells and improves external validity of the pressure-based microbiological challenge study [ 12 , 34 ]. The 10-fold diluted samples were spread-plated onto TSA + YE, aerobically incubated at 37 °C for 48 h, and the colony-forming units (CFU) were calculated based on the Bacteriological Analytical Methods (BAM) of the U.S. Food and Drug Administration [ 35 ] using a Quebec colony counter.…”

Section: Methodsmentioning

confidence: 99%

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Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid

George

Aras

Kabir

et al. 2020

IJERPH

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Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against Staphylococcus aureus. Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% v/v) were applied for the reduction in four-strain mixture of S. aureus in HEPES buffer at 4 and 40 °C for up to 7 min. Results were statistically analyzed by ANOVA and D-values were additionally calculated using best-fitted linear model. Prior to exposure to treatments at 4 °C, counts of the pathogen were 7.95 ± 0.4 log CFU/mL and were reduced (p < 0.05) to 6.44 ± 0.3 log CFU/mL after 7 min of treatment at 450 MPa. D-value associated with this treatment was 5.34 min (R2 = 0.72). At 40 °C, counts were 8.21 ± 0.7 and 5.77 ± 0.3 log CFU/mL before and after the 7-min treatments, respectively. D-value associated with 40 °C treatment was 3.30 min (R2 = 0.62). Application of the antimicrobials provided additional pathogen reduction augmentation for treatments < 5 min. The results of the current study could be incorporated for meeting regulatory requirements such as Food Code, HACCP, and Preventive Control for Human Food of Food Safety Modernization Act for assuring microbiological safety of products against this prevalent pathogen of public health concern.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid

George

Aras

Kabir

et al. 2020

IJERPH

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“…The addition of yeast extract had been proposed in the past to minimize the acid stress to the microorganisms [ 21 , 35 ]. The inoculated TSB + YE for B. atrophaeus and B. amyloliquefaciens were incubated statically at 32 °C for 24 h [ 36 ]. For G. stearothermophilus , the TSB + YE was incubated at 55 °C for 24 h [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…These sub-cultured bacterial suspensions were further used to prepare the endospore suspension based on the method previously validated and utilized for pressure-based pasteurization of bacterial endospores [ 36 , 38 ]. In short, 0.5 mL of each sub-cultured suspension, for each bacterium separately, was aseptically transferred and spread-plated onto the surface of nutrient agar (Difco, Becton Dickinson) supplemented with 0.6% yeast extract and further supplemented with 10 mg/L (i.e., 10 ppm (parts per million)) of MnSO 4 ·H 2 O (VWR International, Radnor, PA, USA).…”

Section: Methodsmentioning

confidence: 99%

Synergistic Effects of Nisin, Lysozyme, Lactic Acid, and CitricidalTM for Enhancing Pressure-Based Inactivation of Bacillus amyloliquefaciens, Geobacillus stearothermophilus, and Bacillus atrophaeus Endospores

et al. 2021

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The inactivation of bacterial endospores continues to be the main curtailment for further adoption of high-pressure processing in intrastate, interstate, and global food commerce. The current study investigated the effects of elevated hydrostatic pressure for the inactivation of endospore suspension of three indicator spore-forming bacteria of concern to the food industry. Additionally, the effects of four bacteriocin/bactericidal compounds were studied for augmenting the decontamination efficacy of the treatment. Elevated hydrostatic pressure at 650 MPa and at 50 °C was applied for 0 min (untreated control) and for 3, 7, and 11 min with and without 50K IU of nisin, 224 mg/L lysozyme, 1% lactic acid, and 1% CitricidalTM. The results were statistically analyzed using Tukey- and Dunnett’s-adjusted ANOVA. Under the condition of our experiments, we observed that a well-designed pressure treatment synergized with mild heat and bacteriocin/bactericidal compounds could reduce up to >4 logs CFU/mL (i.e., >99.99%) of bacterial endospores. Additions of nisin and lysozyme were able, to a great extent, to augment (p < 0.05) the decontamination efficacy of pressure-based treatments against Bacillus amyloliquefaciens and Bacillus atrophaeus, while exhibiting no added benefit (p ≥ 0.05) for reducing endospores of Geobacillus stearothermophilus. The addition of lactic acid, however, was efficacious for augmenting the pressure-based reduction of bacterial endospores of the three microorganisms.

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“…It is also possible to test different inactivation approaches, such as the combination of PATS treatments with antimicrobial compounds. Daryaei and others () evaluated the possibility of coupling PATS (600 MPa, 105 °C) with different antimicrobial compounds (applied prior to the treatment) for the inactivation of B. amyloliquefaciens spores. Among the different compounds tested, chitosan alone, or in combination with licorice extract or sodium dodecyl sulfate, was the most effective for spore inactivation by PATS.…”

Section: Novel Sterilization Technologiesmentioning

confidence: 99%

Application of High Pressure with Homogenization, Temperature, Carbon Dioxide, and Cold Plasma for the Inactivation of Bacterial Spores: A Review

Lopes

Mota

Gomes

et al. 2018

Comp Rev Food Sci Food Safe

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Formation of highly resistant spores is a concern for the safety of low-acid foods as they are a perfect vehicle for food spoilage and/or human infection. For spore inactivation, the strategy usually applied in the food industry is the intensification of traditional preservation methods to sterilization levels, which is often accompanied by decreases of nutritional and sensory properties. In order to overcome these unwanted side effects in food products, novel and emerging sterilization technologies are being developed, such as pressure-assisted thermal sterilization, high-pressure carbon dioxide, high-pressure homogenization, and cold plasma. In this review, the application of these emergent technologies is discussed, in order to understand the effects on bacterial spores and their inactivation and thus ensure food safety of low-acid foods. In general, the application of these novel technologies for inactivating spores is showing promising results. However, it is important to note that each technique has specific features that can be more suitable for a particular type of product. Thus, the most appropriate sterilization method for each product (and target microorganisms) should be assessed and carefully selected.

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Lethality enhancement of pressure-assisted thermal processing against Bacillus amyloliquefaciens spores in low-acid media using antimicrobial compounds

Cited by 16 publications

References 50 publications

Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid

Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid

Synergistic Effects of Nisin, Lysozyme, Lactic Acid, and CitricidalTM for Enhancing Pressure-Based Inactivation of Bacillus amyloliquefaciens, Geobacillus stearothermophilus, and Bacillus atrophaeus Endospores

Application of High Pressure with Homogenization, Temperature, Carbon Dioxide, and Cold Plasma for the Inactivation of Bacterial Spores: A Review

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