LETM1 (leucine zipper-EF-hand-containing transmembrane protein 1) silence reduces the proliferation, invasion, migration and angiogenesis in esophageal squamous cell carcinoma via KIF14 (kinesin family member 14)

Zhao, Qiang; Chen, Sheng; Lu, Chang

doi:10.1080/21655979.2021.1982275

Cited by 9 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the DEGs that were down to virtually 0 normalized counts, B-myb (also known as Mybl2 ) was an already recognized T cell effector player of transition into memory state (Powzaniuk et al, 2001; Chen et al, 2018), while others were newly described in this context, e.g. Ttk , the gene for Thymidine kinase 1, an IL-2 induced kinase regulating cell cycle progression of T cells (Schmandt et al, 1994); Kif14 , the gene for Kinesin Family Member 14, a positive regulator of cell cycle (Yang et al, 2019; Zhao et al, 2021); and Lrr1 , the gene for Leucine-Rich Repeat Protein 1, an inhibitor of 4-1BB signalling (Jang et al, 2001) (Fig. 3A-B).…”

Section: Resultsmentioning

confidence: 99%

Improved memory CD8 T cell response to delayed vaccine boost is associated with a distinct molecular signature

Natalini

Simonetti

Favaretto

et al. 2022

Preprint

View full text Add to dashboard Cite

Although the prime/boost interval can impact vaccine responses, the criteria for deciding its time length are poorly defined. To address this, we examined CD8 T cell responsiveness to boost in a BALB/c mouse model of intramuscular (i.m.) vaccination by priming with HIV-1 gag-encoding Chimpanzee adenovector, and boosting with HIV-1 gag-encoding Modified Vaccinia virus Ankara. We found that boost was more effective at day(d)100 than at d30 post-prime, as evaluated at d45 post-boost by multi-lymphoid organ assessment of gagspecific CD8 T cell frequency, CD62L-expression (as a guide to memory status) and in vivo killing. RNA-sequencing of splenic gag-primed CD8 T cells at d100 revealed a quiescent, but highly responsive signature, that trended toward a central memory (CD62L+) phenotype. Interestingly, gag-specific CD8 T cell frequency selectively diminished in the blood at d100, relative to the spleen, lymph nodes and bone marrow. These results move forward the rational design of prime/boost intervals.

show abstract

Section: Resultsmentioning

confidence: 99%

Improved memory CD8 T cell response to delayed vaccine boost is associated with a distinct molecular signature

Natalini

Simonetti

Favaretto

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Knockdown of LETM1 inhibits cell proliferation, migration, invasion and tumor spheroid formation in prostate cancer cells [27]. The inhibiting effects of LETM1 silencing on malignant characteristics of cancer cells have also been determined in colorectal cancer [10], hepatocellular carcinoma [11], esophageal squamous cell carcinoma [28], bladder cancer [29], and so on. Similarly, with previous studies, silencing of LETM1 inhibited the malignant characteristics of endometrial cancer cells, evidenced by decreased cell viability, colony formation, migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial protein LETM1 and its-mediated CTMP are potential therapeutic targets for endometrial cancer

et al. 2022

View full text Add to dashboard Cite

Leucine zipper/EF hand-containing transmembrane-1 (LETM1) is an important mitochondrial protein, while its function in endometrial cancer remains unknown. This study aimed to explore the function of LETM1 in endometrial cancer and reveal the underlying mechanisms involving carboxy-terminal modulator protein (CTMP). Immunohistochemistry was performed to detect the expression of LETM1 and CTMP in normal, atypical hyperplastic and endometrial cancer endometrial tissues. LETM1 and CTMP were silenced in two endometrial cancer cell lines (ISK and KLE), which were verified by western blot. Cell viability, colony number, migration and invasion were detected by cell counting kit-8, colony formation, wound healing and trans-well assays, respectively. A xenograft mouse model was established to determine the antitumor potential of LETM1/CTMP silencing in vivo. In addition, CTMP was overexpressed to evaluate its regulatory relationship with LETM1 in endometrial cancer cells. The expression of LETM1 and CTMP proteins were higher in endometrial cancer tissues than atypical hyperplastic tissues and were higher in atypical hyperplastic tissues than normal tissues. LETM1 and CTMP were also upregulated in ISK and KLE cells. Silencing of LETM1 or CTMP could decrease the viability, colony number, migration and invasion of endometrial cancer cells and the weight and volume of tumor xenografts. In addition, CTMP was downregulated by LETM1 silencing in KLE cells, and its overexpression enhanced the malignant characteristics of si-LETM1-transfected KLE cells. Silencing of LETM1 inhibits the malignant progression of endometrial cancer through downregulating CTMP.

show abstract

“…The cells were wiped out from the upper chamber, and the invaded cells got fixed with 4% paraformaldehyde and stained with 0.05% crystal violet. The invasive cells were observed and totaled via an inverted microscope [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…Before probing with primary antibodies against KAP-1 (dilution 1:10,000; ab109545, Abcam), MMP9 (dilution 1:1,000; ab76003, Abcam), MMP12 (dilution 1:1,000; ab52897, Abcam), DDX5 (dilution 1:10,000; ab126730, Abcam), β-catenin (dilution 1:1,000; ab16051, Abcam), c-myc (dilution 1:1,000; ab32072, Abcam), cyclin D1 (dilution 1:200; ab16663, Abcam), and GAPDH (dilution 1:2,500; ab9485, Abcam) at 4°C overnight, the membranes were impeded with 5% skimmed milk. On the following day, the membranes were exposure to horse radish peroxidase (HRP)-labeled secondary antibody (dilution 1:5,000; ab6721, Abcam) for 2 h. An enhanced chemiluminescence-detection kit (Thermo Fisher Scientific, Wilmington, USA) was adopted to observe the immune signals and the protein expression was normalized to endogenous GAPDH [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Knockdown of KRAB domain-associated protein 1 suppresses the proliferation, migration and invasion of thyroid cancer cells by regulating P68/DEAD box protein 5

2022

View full text Add to dashboard Cite

KRAB domain-associated protein 1 (KAP-1) has been reported to be an oncogene in diverse tumors. KAP-1 was found to have abundant existence in malignant thyroid tissues, but its role in thyroid cancer hasn’t been elucidated clearly. This study was carried out to explore the role of KAP-1 in thyroid cancer, and to clarify its molecular mechanism. The expressions of KAP-1 and P68/DEAD box protein 5 (DDX5) were assessed under the help of qRT-PCR and western blot. Then, we downregulated KAP-1 or upregulated DDX5 by cell transfection in TPC-1 cells. A series of cellular experiments on proliferation, apoptosis, migration and invasion were conducted with CCK-8, EdU, TUNEL, wound-healing and Transwell assays. Besides, the relationship between KAP-1 and DDX5 was verified by co-immunoprecipitation (Co-IP). The results showed that both of KAP-1 and DDX5 were upregulated in thyroid cancer cells. Loss-of-function experiments revealed that KAP-1 knockdown imparted suppressive effects on cell proliferation, migration and invasion, but promoted cell apoptosis. Additionally, KAP-1 was demonstrated to interact with DDX5 and positively regulate DDX5 expression. The following rescued experiments exhibited that the inhibitory effects of KAP-1 knockdown on cellular activities of thyroid cancer and Wnt/β-catenin signaling were also partly reversed by DDX5 overexpression. Moreover, activation of Wnt/β-catenin signaling retarded the anti-tumor activity of KAP-1 knockdown. In conclusion, the data in this study disclosed that KAP-1 silence helped to repress the cell proliferation, migration and invasion by degrading DDK5, so as to hinder the development of thyroid cancer.

show abstract

LETM1 (leucine zipper-EF-hand-containing transmembrane protein 1) silence reduces the proliferation, invasion, migration and angiogenesis in esophageal squamous cell carcinoma via KIF14 (kinesin family member 14)

Cited by 9 publications

References 30 publications

Improved memory CD8 T cell response to delayed vaccine boost is associated with a distinct molecular signature

Improved memory CD8 T cell response to delayed vaccine boost is associated with a distinct molecular signature

Mitochondrial protein LETM1 and its-mediated CTMP are potential therapeutic targets for endometrial cancer

Knockdown of KRAB domain-associated protein 1 suppresses the proliferation, migration and invasion of thyroid cancer cells by regulating P68/DEAD box protein 5

Contact Info

Product

Resources

About