Letrozole induced low estrogen levels affected the expressions of duodenal and renal calcium-processing gene in laying hens

Li, Qiao; Zhao, Xingkai; Wang, Shujie; Zhou, Zhenlei

doi:10.1016/j.ygcen.2017.10.005

Cited by 17 publications

(16 citation statements)

References 35 publications

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“…The intestinal transcellular pathway occurs in 3 steps: apical Ca 2+ entry, intracellular Ca 2+ transport and basal active Ca 2+ extrusion (Wasserman and Taylor, 1966; Coty and Conkey, 1982). We did not detect TRPV6 in the current study at any age, although previous studies have reported its expression in the intestine of the laying hen (Yang et al, 2011; Jonchère et al, 2012; Li et al, 2018). This observation is in agreement with the absence of a TRPV6 expression in the chicken RNAseq data and in growing broiler chickens, despite the detection of an immunoreactive TRPV6 protein in the chicken's small intestine (Proszkowiec-Weglarz and Angel, 2013; Huber et al, 2015; Juanchich et al, 2018; Proszkowiec-Weglarz et al, 2019).…”

Section: Discussioncontrasting

confidence: 73%

Candidate genes of the transcellular and paracellular calcium absorption pathways in the small intestine of laying hens

et al. 2019

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To meet the high calcium (Ca) demand during eggshell biomineralization (2 g of Ca per egg), laying hens develop specific metabolic regulations to maintain Ca homeostasis. The intake of Ca, its solubilization, and absorption capacity are enhanced at sexual maturity (SM). A better knowledge of the intestinal Ca transporters involved in their variations at this stage could indicate new nutritional strategies to enhance Ca digestive utilization. Transcellular Ca absorption pathway and its major player calbindin-D 28 K (CALB1) mediate a saturable transport, which has been extensively described in this model. Conversely, a contribution by the paracellular pathway involving non-saturable Ca transport through intercellular tight junction has also been suggested. The aim of the present study was to identify candidate genes of these two pathways and their patterns of expression, in immature pullets (12, 15, and 17 wk old) and mature laying hens (23 wk old) in the duodenum, jejunum, and ileum. Using RT-qPCR, this study identifies 3 new candidate genes for transcellular, and 9 for paracellular Ca transport. A total of 5 candidates of the transcellular pathway, transient receptor potential cation channels subfamily C member 1 (TRPC1) and M member 7 (TRPM7); CALB1 and ATPase plasma membrane Ca2+ transporting 1 (ATP2B1) and ATPase plasma membrane Ca2+ transporting 2 (ATP2B2) were enhanced with age or after SM in the duodenum, the jejunum or all 3 segments. A total of 4 candidates of the paracellular pathway Claudin 2 (CLDN2) and tight junction proteins 1, 2, and 3 (TJP1, TJP2 and TJP3) increased in the small intestine after SM. Additionally, CALB1, ATP2B2, and CLDN2 were overexpressed in the duodenum or the jejunum or both segments after SM. The enhanced expression of candidate genes of the paracellular Ca pathway after SM, supports that the non-saturable transport could be a mechanism of great importance when high concentrations of soluble Ca are observed in the intestinal content during eggshell formation. Both pathways may work cooperatively in the duodenum and jejunum, the main sites of Ca absorption in laying hens.

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Section: Discussioncontrasting

confidence: 73%

Candidate genes of the transcellular and paracellular calcium absorption pathways in the small intestine of laying hens

et al. 2019

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“…Overall, LZ treatment in pullets increased cortical bone area and cBMD at 6 and 9 woa compared to untreated animals ( Li et al., 2019 ). Meanwhile, plasma calcium concentration was lower in LZ treated hens ( Deng et al., 2010 ), with a reduction in calcium-binding protein in the duodenum ( Li et al., 2018 ) and a decline in estrogen receptors ( Deng et al., 2010 ; Li et al., 2018 ). Taken together, these studies demonstrate that higher levels of E 2 are associated with medullary bone formation, while lower levels support the formation of cortical bone.…”

Section: Discussionmentioning

confidence: 99%

Bone and eggshell quality throughout an extended laying cycle in three strains of layers spanning 50 years of selection

Hanlon¹,

Takeshima²,

Kiarie³

et al. 2022

Poultry Science

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Decades of intensive genetic selection in commercial layers has resulted in earlier maturation, while sustaining high production rates to 100 wks of age ( woa ). To support eggshell formation while maintaining a healthy skeletal frame, substantial adaptations of calcium metabolism in the hen are necessary. Thus, skeletal growth, bone density, and egg quality were compared in 3 strains of layers, with the Lohmann LSL-lite as the current commercial strain, the heritage Shaver white leghorn as the mid-2000s strain, and the white-leghorn derived Smoky Joes as the non-selected 1960s strain. Tibia and Femur (n = 4/strain) were collected at 12, 17, 20, 25, 45, 60, 75, and 100 woa. Bones were measured and weighed, with bone mineral density assessed within medullary ( mBMD ) and cortical ( cBMD ) regions of the tibia using micro-Computed Tomography. Egg analyses including weight, eggshell thickness ( EST ) and eggshell breaking strength ( EBS ), were conducted throughout lay. Blood samples were collected to measure plasma calcium immediately prior to lay (18 woa) and periodically throughout the laying cycle. Femur and tibia weight, or size, did not increase beyond 12 woa, indicating that all hens reached maximum skeletal size by this time. An interaction ( P = 0.005) was observed between strain and tibia mBMD, as all three strains demonstrated an accumulation of medullary bone from 12 to 100 woa. Regarding egg weight, while Lohmann hen eggs displayed the highest quality at 26 woa, an elevation in egg weight in Lohmann and Shaver hens ( P < 0.001) resulted in a decline in EST and EBS over time ( P < 0.01). Yet, at 100 woa, no strain differed in EST or EBS, despite larger variations in cumulative egg numbers ( P < 0.001). Plasma calcium levels were significantly elevated between the immature state and peak of lay but remained unchanged throughout lay in all strains. In conclusion, our results show that although genetic selection of layer hens resulted in tremendous improvement in productivity, no detrimental effects on cBMD or mBMD were observed throughout an extended laying period up to 100 woa.

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“…The highest expression levels of TRPV6 mRNA were found in the duodenum of laying hens, followed by the jejunum and ileum (Yang et al, 2011). The protein expression levels of CaBP-D28k and PMCA1b were higher in the duodenum than in the jejunum and ileum (Sugiyama et al, 2007;Li et al, 2018). Vitamin D regulates Ca transporter gene expression levels after binding VDR.…”

Section: Introductionmentioning

confidence: 94%

“…CaBP-D9k is found in the small intestine of mammals, whereas CaBP-D28k is expressed in the poultry intestine. Four Ca transporter genes (i.e., TRPV6, CaBP-D28k, PMCAlb, and NCX1) were successfully cloned in the small intestine of laying hens (Sugiyama et al, 2007;Yang et al, 2011;Li et al, 2018). The highest expression levels of TRPV6 mRNA were found in the duodenum of laying hens, followed by the jejunum and ileum (Yang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

1,25-Dihydroxycholecalciferol Improved the Growth Performance and Upregulated the Calcium Transporter Gene Expression Levels in the Small Intestine of Broiler Chickens

Wang

et al. 2022

J. Poult. Sci.

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contributed equally to this work. 1,25-Dihydroxycholecalciferol (1,25-(OH)2-D3) is the final active product of vitamin D. This study aimed to investigate the effects of 1,25-(OH)2-D3 on growth performance, bone development, and calcium (Ca) transporter gene expression levels in the small intestine of broiler chickens. On the day of hatching, 140 female Ross 308 broilers were randomly allotted into two treatments with five replicates (14 birds per replicate). Two levels of 1,25-(OH)2-D3 (0 and 1.25 µg/kg) were added to the basal diet without vitamin D. Results showed that the addition of 1.25 µg/kg 1,25-(OH)2-D3 increased the average daily feed intake and the average daily gain and decreased the feed conversion ratio and mortality in 1-to 19-day-old broiler chickens compared with the basal diet without vitamin D (P < 0.05). 1,25-(OH)2-D3 also enhanced the length, weight, ash weight, and the percentage contents of ash, Ca, and P in the tibia and femur of broilers (P < 0.05). The mRNA expression levels of the Ca-binding protein (CaBP-D28k) in the duodenum, jejunum, and ileum of 19-day-old broilers increased to 88.1-, 109.1-, and 2.7-fold, respectively, after adding 1,25-(OH)2-D3 (P < 0.05). The mRNA expression levels of the plasma membrane Catransporting ATPase 1b (PMCAlb) in the duodenum and the sodium (Na)/Ca exchanger 1 (NCX1) in the duodenum and the jejunum were also enhanced to 1.57-2.86 times with the addition of 1,25-(OH)2-D3 (P < 0.05). In contrast, the mRNA expression levels of PMCA1b and NCX1 in the ileum and that of vitamin D receptor (VDR) in the small intestine were not affected by 1,25-(OH)2-D3 (P > 0.05). These data indicate that 1,25-(OH)2-D3 upregulated Ca transporter gene transcription and promoted Ca 2+ absorption in the small intestine, especially in the proximal intestine (duodenum and jejunum), thereby improving growth performance and bone mineralization in broiler chickens.

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Letrozole induced low estrogen levels affected the expressions of duodenal and renal calcium-processing gene in laying hens

Cited by 17 publications

References 35 publications

Candidate genes of the transcellular and paracellular calcium absorption pathways in the small intestine of laying hens

Candidate genes of the transcellular and paracellular calcium absorption pathways in the small intestine of laying hens

Bone and eggshell quality throughout an extended laying cycle in three strains of layers spanning 50 years of selection

1,25-Dihydroxycholecalciferol Improved the Growth Performance and Upregulated the Calcium Transporter Gene Expression Levels in the Small Intestine of Broiler Chickens

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