Leucas zeylanica (L.) R. Br. protects ethanol and hydrogen peroxide-induced oxidative stress on hepatic tissue of rats

Abstract

The aim of the study was to evaluate the effect of Leucas zeylanica against oxidative stress on hepatic tissue. Oxidative stress was induced by exposing hepatic tissue to ethanol and Fenton’s reagent (H2O2+FeSO4). The effect of oxidative stress on liver also was evaluated by the determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activity and the levels of lipid peroxide (LPO). The antioxidative activity of L. zeylanica was determined by estimating it ability to inhibit the hepatic levels of lipid peroxide (LPO), as indicator of oxidative stress. Concomitantly, the antioxidant phytochemicals such as polyphenols and flavonoids were assessed against pyrogallol and quercetin standards. The ALT and AST activities and the levels of LPO of hepatic tissue were significantly increased by oxidative stress. L. zeylanica pretreatment, however, significantly repressed the oxidative stress on hepatic tissue, as indicated by the decreased activities of ALT and AST enzymes and levels of LPO. Analyses of the phytochemicals revealed that the extract of L. zeylanica contained substantial amounts of polyphenols (74.32 ± 4.6 µg of pyrogallol equivalent/mg) and flavonoids (15.69 ± 2.2 µg quercetin equivalent/mg of extract). Finally, the results of the present study demonstrated the presence of antioxidant phytochemicals, including polyphenols and flavonoids in L. zeylanica and henceforth conferred protection against ethanol and H2O2-induced oxidative stress on hepatic tissue.DOI: http://dx.doi.org/10.3329/icpj.v2i9.16076 International Current Pharmaceutical Journal, August 2013, 2(9): 148-151

Leucas zeylanica is a Bangladeshi plant with significant medicinal prospect: A review

“…Leaves of L. zeylanica (extract of 100% methanol) had proven its significant potential against oxidative injury caused by hydrogen peroxide and ethanol in liver tissue of rats [40]. An established technique was followed to observe the variation of levels of common liver enzymes [41].…”

“…The extract reported a noteworthy reduction of alanine aminotransferase (~ 13-15%) and aspartate aminotransferase (~ 8-10%). This study also estimated two antioxidant phytoconstituents-flavonoids and polyphenols content as 15.69±2.2 µg QE/mg and 74.32±4.6 µg of PE/mg of extract, respectively by using previously defined procedures [40].…”

Phytochemicals from Leucas zeylanica Targeting Main Protease of SARS-CoV-2: Chemical Profiles, Molecular Docking, and Molecular Dynamics Simulations

“…Phytocompounds possess a wide range of pharmacological activities, and traditional healers have employed plants belonging to the Leucas genus to treat various disease states, indicating an immense potential for the discovery of new lead compounds [ 24 ]. L. zeylanica is a weed commonly referred to as “Ceylon slitwort” and locally known as “Kusha” [ 25 ]. The plant is widely distributed throughout China, India, Bangladesh, Sri Lanka, Thailand, Indonesia, Philippines, Vietnam, Cambodia, Nepal, Myanmar, Malaysia, and New Guinea [ 26 ].…”

Pharmacognostic and Preliminary Phytochemical Evaluation of the leaves of Leucas zeylanica

“…The sap of the leaves is used for sores of eyes and nostrils [9]. Leaves and flowers are used for jaundice and used in treatment of burning and urination in the frame of traditional medicine [10].…”